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Articles by Sorin APOSTU
Total Records ( 4 ) for Sorin APOSTU
  Sorin APOSTU , Carmen LAZAR , Ancuta Mihaela ROTAR and Mihaela DUMA
  Listeria monocytogenes is widely spread in the environment and represents a potential risk when non-processed, partially processed of fermented food is consumed. The VIDAS L. monocytogenes II test is a rapid screening test and represents an alternative to the long-term alternative identification. This test can be used for the direct screening of L. monocytogenes in the food samples and the environment. Salmonella is one of the main causes of food toxic infection. Salmonella organisms are ubique in the environment and reach the intestine of humans and other mamals, especially domestic and predomestic (rodents), birds, reptiles and even insects.(Ancut,a M. Rotar, S. Apostu, 2009). Detecting Salmonella using classical methods: preinrichment steps, enrichment, isolation and confirmation can last up to 5 days. The screening techniques, based on the imunoenzimatic analysis (IEA) oferring the possibility of simplifying and the accelerating the detection. This way, the automatic IEA Salmonella VIDAS test (SLM) for detecting Salmonella in food samples and environment uses a mixture of capture antibodies with high specificity both against O antigens and H antigens, which allows the detection of both mobile and imobile Salmonella. The VITEK 2 GP and GN cards were developed to satisfy the growing demand for quality control measures within the food industry and provide identification of organisms in just hours, while traditional methods take days to deliver results. The GN card provides automatic identification of the most significant fermenting and non-fermenting Gram -negative bacilli, including several Salmonella species and E. coli O157, in addition to the select agent organisms Brucella melitensis, Francisella tularensis, Burkholderia mallei, urkholderia pseudomallei and Yersinia pestis. The VITEK 2 GP card provides rapid identification of common Gram - positive organisms, including Listeria and Staphylococcus species (bioMerieux Industry).
  Carmen LAZAR , Ancuta Mihaela ROTAR , Sorin APOSTU and Georgiana BUZGAU
  The quality and safety of grocery became a consumers’ right that affects directly the quality of life. Thus, the issues referring to the safety of grocery is in the center of attention of the institutions created for protecting the customers’ best interests. The bio milk can be defined as milk with zero quality defects. This means that the milk corresponds to all quality criteria, respectively nutritional, sanitary, bacteriologic, technologic, sensorial, and psycho-social. The microbiological analyses that are described in the paper have the purpose of monitoring the microbiological parameters listed COMMISSION REGULATION (EC) No 1441/2007 of 5 December 2007 amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs and REGULATION (EC) No 853/2004 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 29 April 2004 laying down specific hygiene rules for food of animal origin. The research was made during the validity period of the 3 types of milk that were studied (eco milk, UHT milk, conventional) respectively the product in the first day of validity, in the middle of the validity period and in the last day of validity. The methods used represented both rapid and classic standardized techniques, using media of enrichment, isolation and confirmation, characteristic to each method. After the realised determinations referring to some microbiological characteristics of the conventional and ecological milk, through the validity period were observed some alterations related to the microbiological quality of milk.
  Sorin APOSTU , Carmen LAZAR and Ancuta M. ROTAR
  Not available
  Carmen LAZAR , Ancuta M. ROTAR , Sorin APOSTU and Mihaela DUMA
  Many bacterial infections are caused by ingestion of contaminated food. Contamination of food often occurs at home, but can be easily prevented. Home hygiene is particularly important for children, elderly people, pregnant women and immuno-compromised people.(Written by Dr. T. M. Wassenaar, 2009) Microorganisms are living organisms, most of them unicellular creatures that can be seen only with a microscope. Microorganisms are just that, microscopic in size. Even though they can only be viewed through a microscope, just a few microbes can multiply and wreak havoc on the human body. (Melissa Conrad Stoppler, MD, 2009). For the purposes of this article, focus will center on pathogenic bacteria (Escherichia coli, Salmonella and Staphylococcus aureus) wich will be identificated using the optical microscope.The optical microscope remains the fundamental tool for phase identification. The optical microscope magnifies an image by sending a beam of light through the objective. Escherichia coli, or E. coli for short, is a very common bacterium. There are hundreds of different strains of E. coli. Some are harmless while others cause serious illness. Nonpathogenic strains of E. coli -- those that do not cause disease - are normal inhabitants of the intestinal tract in humans and animals. (Melissa Conrad Stoppler, M.D., 2009) Salmonella spp. remains a major cause of morbidity and mortality worldwide. Outbreaks of enteric fever remain commonplace in developing countries, whereas nontyphoidal salmonellas are significant in developed countries, with the main presentation that of gastroenteritis.(E. J. Threlfalla, 2008) \Staphylococcus aureus is considered the world’s third most important cause of foodborne illnesses (Tirado and Schimdt, 2001).The ability of S. aureus to grow and produce SEs under a wide range of conditions is evident from the variety of foods implicated in staphylococcal food poisoning (Le Loir et al., 2003) After the analyzing of samples from all food catergories the presence of Escherichia coli was noticed in 40 samples, Staphylococcus coagulase-positive was noticed in 10 samples and Salmonella Group C and Group D was present in 20 samples.
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