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Articles by Son Radu
Total Records ( 13 ) for Son Radu
  Cheah Yoke-Kqueen , John Laurence and Son Radu
  Staphylococcus aureus isolated from the skin surface of the athletes and their training environment in Malaysia were characterized for their antimicrobial susceptibility and Random Amplified Polymorphic DNA (RAPD). A total of 19 types of antibiotics used in this study and all the isolates were resistant to nalidixic acid and trimethoprim and showed Multiple Antibiotic Resistant (MAR) indexes, ranging from 0.11 to 0.68. No methicillin-resistant Staphylococcus aureus (MRSA) was found among the isolates tested in this study. The dendrogram obtained from the results of the RAPD-PCR discriminated the isolates into 4 clusters and 30 single isolates at the level of 100% similarity. Isolates from different individual and the environment were clustered together and colonized by similar strains.
  Chua Kek Heng , Son Radu , Salmah Ismail and Eddy Hasrul Hassan
  A total of 37 Aeromonas isolates were isolated from retail fish in Malaysia wet market. In this study, the bacteria were tested for their haemolytic activity and susceptibility to antimicrobial agents. Twenty one (57%) Aeromonas isolates showed positive haemolysin activity in this study. Six antimicrobial agents were used in this study and all the Aeromonas isolates were found resistant to at least one antibiotic, and 15 of them (41%) were multiresistant. Multiplex polymerase chain reaction was another approach used to detect the existence of chloramphenicol resistance gene in Aeromonas in this study. The chloramphenicol acetyltransferase (cat) gene was detected in 16 Aeromonas isolates. Nine of the Aeromonas isolates (56%) carried cat II gene, while 1 (6%), 2 (13%), and 4 (25%) of the remaining isolates carried cat I, cat III, and cat IV gene respectively.
  Sara Abudafeera , Mariana Nor Shamsudin , Son Radu , Rozita Rusli and Osman Elobied
  Enterohemorrhagic Escherichia coli (EHEC) of serotype O157 causing hemorrhagic colitis is on the increase in many parts of the world, hence specific surveillance of this pathogen is essential for identifying the sources and monitoring its spread. In this study, a total of 22 imported beef isolates of E. coli O157 isolated from wet market in Seri Serdang in Malaysia and 24 imported lamb isolates of E. coli O157 isolated from Center Slaughterhouse in Al-Ain in United Arab Emirates were examined. All the isolates were investigated by randomly amplified polymorphic DNA (RAPD) analysis with one primer: Gen1-50-9 (5?-AGAAGCGATG-3?). The primer generated polymorphisms in most of the isolates of E. coli O157 tested, producing bands ranging from 0.25 to 4.0 kilobases. The RAPD profiles revealed a high level of DNA sequence diversity within E. coli O157 isolates tested.
  Salmah Ismail , Son Radu , Khalifah Sidek , Mohd Azhar Arifffin , Mohammad Nazmul Hasan Maziz , Irwan Hamzah and Mahmood Ameen Abdulla
  There are four groups of balb/c mice with each of it consists of 6 animals. Group 1, 2 and 3 were injected with dexamethasone daily for three days and experimentally infected with Pasteurella multocida, Escherichia coli harboring a recombinant plasmid ABA392 and Staphylococcus aureus respectively. Whereas, group four serve as control. At 24, 48 and 72 hours post-infection a marked increase in total leucocytes counts while differential leucocytes count revealed a marked increase in neutrophils and decrease in lymphocytes and monocytes in all dexamethasone-treated animals and was more predominant in group 1 and 2 post infection in all animals. At 72 hours post-infection, the histological examination reveals haemorrhagic (hemorrhagic septicaemia) features of lungs in dexamethasone-treated mice and infected with P. multocida and the recombinant plasmid ABA392 but not with the dexamethasone-treated and infected with S. aureus indicate that recombinant plasmid ABA392 may harbor a sequence that may code for virulent factor of Pasteurella multocida.
  Endang Purwati , Son Radu , Ahmad Ismail , Cheah Yoke Kqueen and Lesley Maurice
  Listeria monocytogenes isolated from chicken meat were characterized for their antimicrobial susceptibility, plasmid profile, chromosomal polymorphism by RAPD fingerprinting using three primers and to determine whether genetic information coding for antimicrobial resistance in Listeria monocytogenes strain may be carried on conjugative R plasmid. A total of 28 strains of Listeria monocytogenes isolated from 12 (60%) of the sample analysed, were resistant to three or more antimicrobial agents tested. However, none of the isolates were resistant to norfloxacin. Twelve (42.87%) of the Listeria monocytogenes strains contained plasmid DNA bands ranging in size from 2.7 to 54 Kb. Combination of the plasmid profiling and antibiogram could separate the strains into different types. In conjugation studies, tetracycline resistance was transferred from the donor strains L. monocytogenes LMC2KLl.6, LMC6Kl4.2 and LMC20S3.4 to the recipient strains L. monocytogenes LMC7KL4.5, LMC21S3.5 and LMC25K2.1 at frequencies of 2.15 x 10-8, 3.58 x 10-9 and 3 x 10-9, respectively. Vancomycin-resistance was transferred to Listeria monocytogenes LMC2KL1.6 strain at frequency of 2.15 x 10-8 and novobiocin-resistance was transferred to Listeria monocytogenes LMC20S3.4 strain at frequency of 3 x 10-9. Our results demonstrate that RAPD-PCR fingerprinting method is more sensitive than plasmid profiling and antibiotic resistance patterns with respect to individualization of the isolates used in this study.
  Yousr Abdul Hadi , Zaiton Hassan , Gulam Rusul and Son Radu
  A total of 57 Listeria species strains (19 isolates of Listeria monocytogenes and 38 isolates of Listeria innocua ) isolated from different location in the state of Selangor (Seri Serdang, Seri Kembangan, Kajang and Bangi) were characterized by using RAPD-PCR fingerprinting technique. Primer GEN15002 and GEN15009 were chosen whereby it produced reproducible and typeable results in L. monocytogenes (19) and L. innocua (38) isolates examined with the bands sizes ranging from 0.25 to 3.0 kilobase pairs. From the current study, it can be concluded that the RAPD profiles revealed a wide variability and high level of DNA sequence diversity within Listeria species strains tested. RAPD showed a very discriminatory power since several RAPD profiles were obtained among L. monocytogenes and L. innocua isolates and even slight difference between isolates can be detected.
  Chua Kek Heng , Son Radu , Salmah Ismail and Nuruliza Roslan
  A total of 29 samples of Aeromonas veronii biovar sobria and 8 samples of Aeromonas hydrophila were isolated from retail fish in Malaysia wet market. Haemolysin-specific polymerase chain reaction approach was used to screen the -haemolysin gene in these samples. In this study, we found approximately 57% of the Aeromonas samples showed haemolytic activity on agar plate. Six out of the haemolytic Aeromonas spp. and 1 non-haemolytic sample were detected carrying the -haemolysin gene by using PCR. This -haemolysin gene was also detected in Aeromonas veronii biovar sobria in this study which is not being reported in other study.
  Jacintha Sugnaseelan , Salmah Ismail , Lesley Maurice , Gwendolyne Tanil Bulan , Belinda Edwin , Cheah Yok Kqueen and Son Radu
  A total of forty-three Vibrio alginolyticus strains isolated from cockles collected from wet markets were studied for their resistance to antibiotics and plasmid profiles. All isolates were resistant to one or more of the fifteen antibiotics tested. Twenty different antimicrobial resistance patterns were obtained, suggesting that they do not share a common resistance pattern. The multiple antibiotics resistance indexes indicate that most of the V. alginolyticus isolates originated from high-risk sources. The isolates showed high-resistance towards vancomycin (100%), penicillin G (97.7%), bacitracin (95.4%), cephalothin (86.1%), carbenicillin (83.7%), ampicillin (79.1%), erythromycin (79.1%) and tetracycline (55.8%), and low-resistance towards streptomycin (27.9%), chloramphenicol (11.6%), moxalactam/latomoxef (4.7%) and nalidixic acid (2.3%). However, all were susceptible to gentamicin, kanamycin and norfloxacin. Twenty-seven (62.8%) isolates were plasmidless, while sixteen (37.2%) harboured either one or two low molecular weight plasmid(s) ranging between 2.1 and 3.74 MDa. The antibiotic resistance test was more discriminating compared to plasmid profiling and is well suited to characterised the V. alginolyticus strains.
  Ooi Wai Ling , Son Radu , Gulam Rusul , Mohamed Ismail Abdul Karim , Endang Purwati and Samuel Lihan
  A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
  Endang Purwati , Zaiton Hassan , Gulam Rusul , Raha Abdul Rahim and Son Radu
  The objective of present work was to optimize the procedures of FDA and USDA for the isolation of Listeria species in imported frozen beef samples marketed in Malaysia. The modifications consisted of direct analysis or storage of samples at 4°C for 24 h prior to analysis, and enrichment at 30°C or 35°C for 24, 48 and 168 h. For both FDA and USDA modified methods, storage at 4°C for 24 h and pre-enrichment at 24 and 48 h were the most efficient. However, the modified FDA with storage at 4°C for 24 h and pre-enrichment for 24 h (30°C and 35°C) and 48 h (30°C and 35°C) yielded more Listeria species. The rates of isolation were markedly affected with prolonged pre-enrichment incubation up to 168 h. The overall conclusion was that the modified USDA isolation method is beneficial when a limited range of the clinically important Listeria species is sought, whilst the modified FDA is needed to estimate the prevalence of Listeria species in the samples examined.
  Yetti Marlida , Nazamid Saari , Son Radu and Fatimah Abu Bakar
  The degradative activity on various raw starches by enzyme from Synnematous sp., an endophytic fungus was studied. The enzyme hydrolyzed raw starches to produce maltose and glucose. Maximum maltose and glucose were produced from raw rice and tapioca starch were 18.8 and 3.6%, respectively. The yields were dependent on accessibility of granules surface to be attacked by the enzyme. The results of this study suggest that enzyme from Synnematous sp. has potential for the production of glucose and maltose using raw starches as substrates.
  Yuherman , Son Radu , Gulam Rusul , Lum Keang Yeang , Ooi Wai Ling and Jamal Khair
  DNA fingerprinting by PCR amplification of enterobacterial repetitive intergenic consensus (ERIC) and pulsed-field gel electrophoresis (PFGE) were used to compare environmental and clinical isolates of Vibrio cholerae O1 and non-O1. All the V. cholerae O1 and non-O1 isolates were typable using ERIC PCR. Though PFGE generated banding patterns to discriminate the isolates into twelve fingerprints, eight isolates were untypable by PFGE due to consistent degradation of the bacterial DNA. Based on the dendrogram generated from ERIC-PCR method, three of the clinical isolates (C1, C2 and C3) were closely related to environmental isolates (E6 or E10). The results indicate that ERIC-PCR is a very discriminative and efficient method for studying genetic diversity of V. cholerae isolates.
  E. Amghalia , Nagi A. AL-Haj , Mariana N. Shamsudin , Son Radu , Rozita Rosli , V. Neela and Raha A. Rahim
  Multiple drug resistant Staphylococcus aureus is one the most common nosocomial pathogen worldwide. The timely identification of this hospital acquired pathogen and detection of the various antibiotic resistant genes harbored is one of the most important function of the microbiology laboratory. In this study, we report the development of a multiplex PCR system for the diagnosis of S. aureus and the detection of clinically relevant antibiotic resistance genes harbored by some isolates. This system was designed to identify S. aureus at species level and to detect methicillin, gentamycin, erythromycin, vancomycin and mupirocin resistant genes, respectively from a single colony in a single tube reaction. All isolates amplified a 108 bp fragment (conserved in S. aureus) confirming the identity of S. aureus, 23 isolates produced a band at the position of 533 bp, 28 isolates at 139 bp and 30 isolates at 174 bp evidencing the presence of mecA (methicillin or oxacillin resistance), ermA (erythromycin resistance), aac (6`)-aph (2``) (gentamycin resistance) genes. None of the isolates amplified van A (vancomycin resistance) and ileS-2 (mupirocin resistance) genes showing the absence of their resistance in the isolates studied. These genotypic results when compared with classical antibiotic susceptibility tests showed less correlation. Overall, we found a correlation between phenotypic and genotypic methods of 60% for methicillin, 36.7% for gentamycin, 43.3% for erythromycin, 100% for vancomycin and mupirocin. This suggests that classical antibiotic sensitivity test is not accurate, but need to be supplemented with other methods to be applied in a clinical laboratory. The system developed in this study offers a rapid, simple specific and accurate detection of multiple antibiotic resistant genes in clinical S. aureus isolates and thus could be systematically applied as a diagnostic test in clinical microbiology laboratories, facilitating the design and use of antibiotic therapy.
 
 
 
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