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Articles by Somphong Sahaphong
Total Records ( 3 ) for Somphong Sahaphong
  Kandarat Lamchumchang , Wannee Jiraungkoorskul , Somphong Sahaphong , Tawewan Tansatit , Piya Kosai and Raviporn Madarasmi
  This study investigated the influence of dietary calcium supplementation to reduce the toxicity of sublethal lead concentration in Nile tilapia with emphasis on biochemical and histopathological analysis. The values of 24, 48, 72 and 96 h LC50 of lead to tilapia were 247.51, 197.47, 193.36 and 182.38 mg L-1, respectively. Therefore, lead concentration tested in the sublethal experiment was 45 mg L-1, which correspond to 25% of the 96 h LC50. Fish were fed with 0, 20 and 60 mg Ca2+ g-1 food for 30 days. In the gills of Pb treatment, edema, lamellar cell hyperplasia, epithelial lifting, lamellar fusion and aneurysm were observed. In the liver, there were blood congestion in sinusoids, vacuolation of hepatocytes and necrosis. In the kidney, glomerulus’s atrophy, tubular swelling and also necrosis were seen. The structural damages could be correlated to the significant increase (p≤0.05) in aminotransferase activities. Fish fed with Ca2+ supplemented diets showed slightly alteration when compare the only Pb treatment groups. These results indicated that dietary Ca2+ will be protective in reducing Pb burdens in fish exposed to environments contaminated with waterborne Pb.
  Wannee Jiraungkoorskul , Piya Kosai , Somphong Sahaphong , Pukaew Kirtputra , Jasmin Chawlab and Shanida Charucharoen
  Nile tilapia (Oreochromis niloticus), Butterfish (Poronotus triacanthus) and Red-tailed tinfoil barb (Puntius altus), three fish species inhabiting in Southeast Asia freshwater ecosystems were evaluated for their use as pollution biomarkers using the micronucleus (MN) and nuclear abnormalities(NA) tests in erythrocytes. NA shapes were scored into blebbed nuclei (BL), lobed nuclei (LB), notched nuclei (NT) and binuclei (BN). Fish were exposed to lead (Pb), copper (Cu) and cadmium (Cd) for the period of 24, 48, 72 and 96 h. It was observed that, fish species showed significant sensitivity to the different heavy metals treatment. In general, the highest value of both MN and NA cells were significantly increased in the exposure to Pb followed by Cu and Cd. On the other hand, Nile tilapia was the most sensitive to the three heavy metals exposure. The frequencies of each NA shape were found in the all fish species and in the all treatments as following NT>LB>BN>BL. Results of MN and NA revealed the highest number after 48 h treatment in all cases and decreased within the longer time exposure. Our results demonstrated the suitability of Nile tilapia for genotoxicity of pollution biomarkers using the micronucleus and nuclear abnormalitiestest.
  Wannee Jiraungkoorskul , Somphong Sahaphong , Piya Kosai and Myung-Huk Kim
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