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Articles by Sobri Hussein
Total Records ( 4 ) for Sobri Hussein
  Sobri Hussein , Rusli Ibrahim , Anna Ling Pick Kiong , Nor`aini Mohd Fadzillah and Siti Khalijah Daud
  The first successful micropropagation protocol for an important recalcitrant medicinal plant in Southeast Asia, Eurycoma longifolia Jack or Tongkat Ali as known in Malaysia was developed. In attempts to establish the somatic embryos of E. longifolia, the potential of cotyledon, zygotic embryo, leaf, petiole, stem and taproot in forming embryogenic callus were examined in the basal Murashige and Skoog (MS) medium supplemented with different auxins at various concentrations. Only cotyledon explants were able to form embryogenic callus in the presence of 1.0 mg L -1 (w/v) of 2,4-dichlorophenoxyacetic (2,4-D). A higher yield (60%) of embryogenic callus was obtained when the Type 4 method dissected cotyledon explants were cultured in basal MS medium containing 0.5 mg L -1 (w/v) of kinetin and 1.0 mg L -1 (w/v) of 2,4-D. The highest number of somatic embryos (45±2) was observed in the same medium formulation with the addition of 1.0 g L -1 (w/v) activated charcoal. Subsequent transfer of these mature somatic embryos in basal MS media supplemented with 1.0 mg L -1 (w/v) of kinetin produced a 90% of plantlet regeneration. The differences between the embryogenic and non-embryogenic callus were also determined based on the histological studies.
  Anna Ling Pick Kiong , Hor Hong Huan and Sobri Hussein
  A callogenesis protocol for Melaleuca alternifolia was developed in this study. The effects of different types of auxins, activated charcoal and combination of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin at various concentrations on the callus induction from leaf explants of M. alternifolia were investigated in order to determine the optimum callus induction and maintenance medium. The callus growth curve under the best callus maintenance medium was also studied. Calli were induced from leaf explants cultured on Murashige and Skoog (MS) medium incorporated with 1, 3 and 5 mg L-1 of 2,4-D, Indole-3-butyric Acid (IBA), Naphthaleneacetic acid (NAA) or picloram as well as MS medium with the combination of 3 mg L-1 2,4-D and 1, 2 or 3 mg L-1 kinetin, respectively. The calli developed from different types of plant growth regulators were varied in their morphological appearances. The maximum callogenesis response was obtained using the MS medium supplemented with 3 mg L-1 2,4-D. The control medium and the medium incorporated with NAA did not produce any callus. Addition of activated charcoal at 0.05 and 0.1% did not assist in the callogenic response, though it helped in removing the pigments and phenolic compound accumulations in the culture medium. The calli initiated from the best induction medium could not be maintained in the fresh new medium containing the same auxin composition. However, calli were successfully maintained in MS medium containing the combination of 3 mg L-1 2,4-D and 2 mg L-1 kinetin. The callus maintained in this treatment showed a sigmoid growth pattern and reached maximum the growth rate after three to five weeks upon cultivation.
  Sobri Hussein , Rusli Ibrahim and Anna Ling Pick Kiong
  Anna Ling Pick Kiong , Leong Sok Wan , Sobri Hussein and Rusli Ibrahim
  The effects of different explants and the position of explants on the induction of callus and somatic embryos were studied on Citrus sinensis. The explants; cotyledon, mature zygotic embryo, in vitro leaf and in vitro stem were cultured on full-strength MS basal medium supplemented with 2,4-dichlorophenoxy-acetic acid (2,4-D), 3,6-Dichloro-2-methoxybenzoic acid (dicamba), 1-Naphthaleneacetic acid (NAA) and 4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid (picloram). Direct and indirect somatic embryogenesis was observed from the cotyledon and mature zygotic embryo explants. Direct somatic embryo were formed from the cotyledon and mature zygotic embryos explants cultured on the medium containing 3 and 5 mg L-1 NAA. Indirect somatic embryogenesis was also observed in the cotyledon explants cultured on the medium supplemented with 2 mg L-1 dicamba as well as the mature zygotic embryo explant cultured on the medium with 3 and 5 mg L-1 picloram. The somatic embryo formed through direct or indirect somatic embryogenesis contained the similar stage, namely the globular, heart, torpedo, cotyledonary before developed into mature embryo. All these mature somatic embryos were further regenerated into in vitro plantlet on the medium containing 5 mg L-1 NAA after 3 weeks culture. In vitro leaf and stem explants were unable to induce somatic embryo from the four types of Plant Growth Regulators (PGRs) studied. Formation of root or shoot was observed from the in vitro stem incubated on the medium supplemented with 1 mg L-1 2,4-D; 1 and 2 mg L-1 picloram; 1, 2 and 5 mg L-1 NAA and also Murashige and Skoog (MS) medium without PGRs. Cotyledon explants which cut horizontally into three parts and cultured into medium containing 4 mg L-1 2,4-D. All the explants induced non-embryogenic callus after 3 weeks culture.
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