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Articles by Sobri Hussein
Total Records ( 2 ) for Sobri Hussein
  Sobri Hussein , Rusli Ibrahim and Anna Ling Pick Kiong
  This study reported the first successful adventitious shoots regeneration from the root and stem explants of E. longifolia. Generally, stem explants appeared to have a lower regeneration capability when treated with the cytokinins alone compared to the root explants. In the root explant, the best medium formulation determined was basal Juglans medium (DKW) supplemented with 1.0 mg L-1 of kinetin + 1.0 mg L-1 of zeatin whereas in the stem explants, basal woody plant medium (WPM) enriched with 2.0 mg L-1 of BAP + 2.0 mg L-1 of zeatin was found to be the best medium formulation in increasing the regeneration rate. Stem and root explants that were 2.0 cm distant from one another has been identified as the most suitable position for attaining the maximum percentage of adventitious shoots formation. Rootlets induction of adventitious shoots formed was also achieved and the highest induction rate was attained in basal MS medium supplemented with 0.5 mg L-1 of Indole-3-butyric acid. Acclimatization of the in vitro plantlets produced survived well with no morphological differences from the parent plants after two months of transplantation to the soil.
  Anna Ling Pick Kiong , Then Chia Nee and Sobri Hussein
  Murashige and Skoog (MS) nutrient media supplemented with auxins 2,4-dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA), Indole-3-acetic acid (IBA) and 4-amino-3, 5, 6-trichloro picolinic acid (picloram) each at the concentrations of 1, 3 and 5 mg L-1 were evaluated for their effects on the callus induction from the leaf explants of Ficus deltoidea. The best calli with healthy morphology and growth was obtained from explants cultured on MS media supplemented with 3 mg L-1 picloram. Friable calli with white, greenish or yellowish colour were obtained after 10 days upon culturing. 2, 4-D and IBA induced morphologically unhealthy calli, whereas cultured explants gave a negative response to MS media supplemented with various levels of NAA. For callus maintenance, the study indicated that media supplemented with 1 mg L-1 picloram gave the best growth rate of calli and was the most suitable callus maintenance medium to be used.
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