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Articles by Sobhy Abdel-Shafy
Total Records ( 5 ) for Sobhy Abdel-Shafy
  Abdullah Daria ALanazi , Swaid Abdullah , Chris Helps , Richard Wall , Robert Puschendorf , Samir Abdelkreem ALHarbi , Sobhy Abdel-Shafy and Raafat Mohamed Shaapan
  Background and Objectives: Domestic animals, including camels, in Saudi Arabia suffer from various diseases, among which tick-borne infections are important because they reduce the productivity of these animals. However, knowledge of tick-borne pathogens in camels in Saudi Arabia is very limited, so the aims of this study were to quantify the abundance and distribution of tick species infesting camels from different districts of Riyadh province and use molecular tools to detect tick-borne pathogens in both the ticks and blood samples. Materials and Methods: A total of 218 ticks were collected from 116 camels from the 5 districts of Riyadh. The ticks and camel blood samples were analyzed for Borrelia, Babesia and Theileria pathogens using conventional and real-time PCR. Results: The results showed that five different tick species were identified. Majority of the ticks were Hyalomma dromedarii (70.6%), which were collected from camels in all 5 districts. This was followed by Hyalomma impeltatum species (25.2%), which was again found in all the districts. The other species found were Hyalomma anatolicum, Haemaphysalis sp. and Rhipicephalus turanicus. The only one H. dromedarii tick was positive for Theileria sp. DNA. Although the sample size and the area of tick collection were limited, the data suggest that the prevalence of pathogens in the Riyadh province, Saudi Arabia is relatively low. Conclusion: The study provides useful preliminary data to inform future full-scale country-wide surveys.
  Sobhy Abdel-Shafy , Mona S. Mahmoud and Mohamed M. Abdel-Aziz
  The study aim to evaluate crude and two fractionated gut extract antigens of engorged females of Hyalomma dromedarii for protection against tick-feeding. To obtain the two fractionated gut extract antigens (FGE1 and FGE2) from Crud Gut Extract (CGE), gel filtration was used. CGE, FGE1 and FGE2 were used as immunogens for rabbits with Freund`s adjuvant. Six immunized and two control groups were challenged with adult and larvae of H. dromedarii (3 immunized and 1 control for each stage). Results showed that the efficacy of immunogens against adult (E%) showed that FGE1 recorded the highest immune effects on the adults (64.97%) followed by CGE which recorded (30.97%) and the lowest protection recorded with FGE2 (21.57%), while E% of CGE, FGE1 and FGE2 were 85.01, 82.13 and 70.1%, respectively, on larvae. The results of Enzyme Linked Immunuosorbent Assay (ELISA) revealed that the level of antibodies for all tested antigens increased gradually until the date of challenge with ticks and then declined. Western blot technique for both adults and larvae showed that the antigen CGE revealed two reactive bands at molecular weights of 50 and 34 kDa before challenge with ticks. These bands still to be reactive after challenge with ticks. Meanwhile, before challenge, the antigen FGE1 exhibited 3 reactive bands with molecular weights 145.89, 61.09 and 24 kDa and one band at molecular weight of 34 kDa after challenge with ticks. Moreover, the antigen FGE2 had only one reactive band at molecular weight of 34 kDa before challenge with ticks and two reactive bands at molecular weight of 34 and 24 kDa after challenge with ticks.
  Abdullah D. Alanazi , Hamdan I. Al-Mohammed , Mohamed S. Alyousif , Robert Puschendorf and Sobhy Abdel-Shafy
  Background and Objectives: Ticks are the major vectors of different pathogens to humans and animals. The aim of this study was to determine the current status of tick species infesting domestic and wild animals and their seasonal population dynamics. Materials and Methods: This survey on tick species composition was carried out in Riyadh province, Saudi Arabia between January and December, 2017. Ticks were collected from the bodies of 18 domestic and wild animals. A total of 11,587 ixodid ticks (6,247 females; 4,585 males; 755 nymphs) were collected and preserved in 70% alcohol and then identified as to species. Results: The identified specimens belonged to ten species, including two genera of Hyalomma and Rhipicephalus. In domestic animals, the Hyalomma species comprised 68.3% and Rhipicephalus species 31.7%. Hyalomma dromedarii (39.9%) was the most common and abundant species, followed by Rhipicephalus turanicus (34.9%), while Rhipicephalus sanguineus was the most common species in wild hosts (83.0%). In addition, tick populations were most prevalent during summer and spring (36.0 and 31.6%, respectively). Conclusion: The results of this study showed that some tick species have expanded their distribution elsewhere to their previously recognised locations in Saudi Arabia. Moreover, the results of this study provide new data that government authorities can implement to take prevention measures for diseases transmitted by ticks.
  Sayeda Sayed Ahmed , Magda Hanna Naroz , Sahar Yassin Abdel-Aziz , Mona Abel-Rahman Awad and Sobhy Abdel-Shafy
  Background and Objective: The bean weevil, Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae) is a serious Neotropical origin insect pest on kidney beans, Phaseolus vulgaris L. and other legume seeds. The objective of this study was to discriminate A. obtectus through morphological, molecular and biological studies. Materials and Methods: All stages of A. obtectus were morphologically described and their biology was performed. The adults of both A. obtectus and Callosobruchus chinensis were characterized by molecular analysis. Results: All the developmental stages were morphologically described. The morphological description of antennae, legs and wings of the adults indicated that the insect is A. obtectus. The partial sequence of COI gene of A. obtectus revealed 99% similarity with A. obtectus that previously recorded in GenBank and located in a separate glade in the phylogenetic tree. The biological parameters such as eggs per female, hatchability and durations of the developmental stages were also estimated. Conclusion: The morphological description, the partial sequence of COI gene and the biological parameters discriminated A. obtectus obviously from C. chinensis.
  Salwa M. Habeeb , Sobhy Abdel-Shafy and Abd El-Ghany A. Youssef
  GC-MSE analysis of the essential oil of fresh fruit peel of Citrus sinensis var. balady recognized two main natural toxic compounds, limonene (83.28%) as hydrocarbon compound and linalool (3.97%) as oxygenated compound. Therefore, the objective of this study was to evaluate its effect on different egg-ages of Hyalomma dromedarii at four concentrations of 1:40, 1:30, 1:20 and 1:15 (oil : ethanol 95%) (v/v). The LC50 values were 1:56, 1: 34, 1: 41, 1: 32, 1: 23, 1: 23, 1:18, 1:14 and 1:11 for egg-ages of 2, 4, 6, 9, 11, 13, 16, 18 and 20 day, respectively. Histological Examination (HE), Scanning Electron Microscopy (SEM) and Sodium dodecyle sulphate polyacrylamide gel electrophoresis (SDS-PAGE) were done on the 9th day old-eggs treated with the essential oil 1:32 (the LC50 value of 9 day old-egg). HE was done on the 11, 12, 13, 14 and 15th day old eggs; SEM was done on the 11, 15 and 17th day old eggs and SDS-PAGE was done on the 10, 11, 12, 13, 14, 15 and 17th day old eggs and compared each with those of control. In control, HE showed that nuclei migrated to the periphery and became part of the cytoplasmic membrane, blastula appears as a complete ring cells. Germ layer form and the later differentiate to different organelles such as opithosoma, ambulatory segment and chelicera…etc. while incase of treated eggs, HE showed that irregular manner of ectoplasmic membrane formed, blastula gathered on one or two sides, the cells of germ layer gather on one side as small or large mass or ring shape. Cells gathered as small masses or finger shape without forming any organelles. SEM revealed that heavy small bulging wrinkles were observed on egg shells of control. These wrinkles changed into large size in treated eggs on the 11th day and disappeared at the following days to become smooth surfaced. SDS-PAGE exhibited 15, 14, 14, 12, 17, 14 and 15 bands for treated eggs on the 10, 11, 12, 13, 14, 15 and 17th day old-eggs, respectively and 14, 15, 16, 19, 17, 19 and 18 bands for control eggs at the same egg-ages. The molecular weights of these bands were different in both control and treated eggs. It was concluded that the essential oil of C. sinensis var. balady has strong toxic effect on eggs of H. dromedarii especially in earlier embryonic development.
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