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Articles by Soad E. Hassan
Total Records ( 3 ) for Soad E. Hassan
  Soad E. Hassan , Nagwa I. Toaleb , Eman E. El Shanawany , Raafat M. Shaapan , Sanaa K.A. Abou-El-Dobal and Ehab Kotb Elmahllawy
  In the present study, crude extract of Toxoplasma gondii tachyzoites were prepared from chicken local isolate and adopted for diagnosis of chicken toxoplasmosis by ELISA. The extract proved success in detection of Toxoplasma antibodies in 275 (78.12%) out of 352 examined free rang chicken serum samples. The important facet of the present study is adoption of crude chicken tachyzoites antigen in diagnosis of human toxoplasmosis, where it proved 100% sensitivity in the detection of Toxoplasma antibodies in all examined cases (61 samples) of human toxoplasmosis. Meanwhile, RH strain antigen, which used to be utilized in diagnosis of human toxoplasmosis, detected only 80% of chicken toxoplasmosis samples. By SDS-PAGE, chicken tachyzoites antigen resolved to 8 bands of molecular weights ranged from 30-116 kDa. Also, RH strain antigen revealed 10 bands with different molecular weights ranged from 23-166 kDa. The immunoreactive bands were detected by immunoblotting assay. One band of 116 kDa was detected in chicken antigen and 28 kDa in RH antigen by infected human sera. While, two bands with molecular weights 116 and 83 kDa and 116 and 30 kDa were detected in chicken tachyzoites and RH antigens, respectively using infected chicken sera. A band of 116 kDa was detected in chicken antigen using both sera, which is mainly responsible for diagnosis of chicken and human toxoplasmosis. In conclusion, chicken tachyzoites antigen based ELISA can be successfully utilized in diagnosis of chicken and human toxoplasmosis.
  Soad E. Hassan , Nagwa I. Toaleb , Raafat M. Shaapan , Eman Hussien Abdel-Rahman and Ehab Kotb Elmahallawy
  To increase diagnostic potency of antigen, its immunogenic component must be isolated. Immuno-affinity column chromatography was adopted to purify immunogenic fraction of Toxoplasma gondii tachyzoites of chicken local isolate. The isolated fraction was characterized by SDS-PAGE, where it exhibited 2 bands of molecular weights 116 and 83 kDa compared with 8 bands of molecular weights ranged from 116-30 kDa in crude antigen. The isolated fraction proved high diagnostic activity compared with crude antigen by ELISA using two fold serial dilutions of infected human and chicken sera. The fraction detected antibodies in 366 out of 406 (90.1%) examined free range chicken serum samples. Also, it proved success in the detection of Toxoplasma antibodies in 30 serum samples of patient infected with T. gondii. The fraction proved 100% sensitivity and 84.4% specificity. Its Positive Predictive Value (PPV), Negative Predictive Value (NPV) and diagnostic accuracy were 85.7, 100 and 91.9%, respectively. In conclusion, the present study introduced novel T. gondii chicken tachyzoites partially purified fraction proved high diagnostic activity of chicken and human toxoplasmosis.
  Soad E. Hassan , Sanaa K.A. Abou-El-Dobal and Eman H. Abdel-Rahman
  Background and Objective: Trichostrongylus colubriformis is a common nematode which infect livestock and can cause accidental infection in humans. In the present study, affinity purification process succeeded in the isolation of a single specific fraction identified by experimentally infected rabbit sera. Methodology: The isolated fraction detected T. colubriformis antibodies in 68 (77.3%) out of 88 examined sheep serum samples. By SDS-PAGE, the isolated fraction showed simple electrophoretic profile and resolved into three bands of molecular weights 74, 85 and 97 kDa. While eight bands of molecular weights ranged from 25.7-189 kDa were detected in crude adult worm extract. In immunoblot assay, the three bands of isolated fraction were reacted with infected rabbit serum compared with four bands of molecular weights 41, 74, 85 and 97 kDa in crude extract. Results: Concerning, humeral immune responses of infected rabbits, the level of IgG antibodies increased to reach (0.526 OD±0.0027) in the first-week post infection. Then decreased to reach (0.34 OD±0.02) at the end of the experiment using ELISA. A significant expression of IL4 was observed and reached the highest level (225±0.012) at second-week post infection. While, IFN-ɣ showed less and nearly constant expression through out the time of the experiment. Conclusion: The partially immuno-affinity purified fraction of T. colubriformis adult antigen proved high diagnostic efficacy in diagnosis of trichostrongylosis in sheep. In addition, T. colubriformis infection stimulates rabbits cellular and humeral immune responses IL4, IFN-γ and IgG which, possibly create some level of protection to re infection.
 
 
 
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