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Articles by Siti Nur Aisyah
Total Records ( 1 ) for Siti Nur Aisyah
  Siti Nur Aisyah , Jefri Maldoni , Irma Sulastri , Weni Suryati , Yuli Marlisa , Lissa Herliana , Lily Syukriani , Renfiyeni Renfiyeni and Jamsari Jamsari
  Background and Objective: Assessment of the plant growth promoting (PGP) properties of a bacteria in vitro required the adjustment of appropriate culture condition. This study was aimed to optimize the culture condition required to stimulate two PGP traits (antifungal activity and indole-3-acetic acid (IAA) production) of phylloplane Serratia plymuthica strain UBCF_13. Materials and Methods: Evaluation of UBCF_13 antifungal activity against F. oxysporum f. sp. glycine was conducted under various modifications (pH and addition of exogenous carbons, Nitrogens as well as metals). This strain IAA production was optimized under various culture durations and L-tryptophan (Trp) concentrations. The resulted IAA were then inoculated to several plant’s seed to evaluate its effect on plant root and shoot growth. Data were analyzed statistically and the significance was further assessed using Duncan’s new multiple range test with a p<0.05. Results: Application of 50 μL cells-free supernatants (CFS) exhibited the highest fungal suppression under pH 5 (23.02%), (NH4)2 SO4 addition (28.25%) and CaCl2 addition (27.14%). The highest IAA concentration (116.09 μg mL1) was obtained from the 48 h culture containing 0.2% L-Trp. The IAA-containing CFS of UBCF_13 stimulated longer growth in root and shoot of Solanaceous plants (chili, tomato and eggplant). Conclusion: Antifungal activity of UBCF_13 was predicted to be dependent on acidic condition and the availability of inorganic nitrogen as well as calcium, while its IAA could be produced in short time with the low amount of Trp.
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