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Articles by Siti Helmyati
Total Records ( 3 ) for Siti Helmyati
  Siti Helmyati , Lily Arsanti Lestari , Odilia Ratna Mayasari , Maria Wigati , Setyo Utami Wisnusanti , Endang Sutriswati Rahayu and Muhammad Juffrie
  Background and Objective: Synbiotic fermented milk with tempeh extract and iron fortification is a diversification of Indonesian local food to overcome anemia. However, consuming excessive iron may increase the pathogen virulence in the gut. Antibacterial agents from the Lactobacillus plantarum Dad 13 and tempeh are assumed to kill the pathogens effectively. To determine the effect of synbiotic fermented milk with tempeh extracts and iron fortification on total Enterobacteriaceae and to measure the antibacterial activity of the milk against Escherichia coli and Staphylococcus aureus. Materials and Methods: There were three groups of synbiotic fermented milk with tempeh extract, with two groups fortified with: (1) NaFeEDTA (SN), (2) FeSO4 (SF) and one group, (3) without fortification (SK). The milk groups were made and stored for 4 and 2 weeks and 1 day before analysis. Specifically, antibacterial activity was examined after the samples were stored f or 2 and 0 weeks while total Enterobacteriaceae was measured after stored for 0, 2 and 4 weeks. Results: The antibacterial activity of the study groups was almost the same against both E. coli or S. aureus (p>0.05). Total Enterobacteriaceae decreased in the longer shelf life (SN Group: week (0 2.26×108), week (2 1.45×106), week-4 estimated <1×105, p<0.05). Conclusion: The addition of iron did not hamper the antibacterial activity of synbiotic fermented milk with tempeh extracts against E. coli and S. aureus. After confirming previous findings related to iron supplementation and anemia, this study further demonstrated the extract’s effectiveness against Enterobacteriaceae.
  Siti Helmyati , Mohammad Juffrie , Endang Sutriswati Rahayu and B.J. Istiti Kandarina
  The role of gut microbiota in human health is highly understandable. Recent research highlights the influence of geographical terms in gut microbiota composition. Unfortunately, baseline data of microbiota composition in children are limited. We compared the number of fecal microbiota of children ages 9-12 years, living in Kulon Progo (KP), Yogyakarta, with same age children in West Lombok (WL) and West Nusa Tenggara. The study was an observational study with comparative design. The subjects were elementary school students, 29 subjects in KP and 27 subjects in WL. Fecal microbiota was analyzed by pour plate culture technique on MRS agar for lactic acid bacteria (LAB) and TBX agar for E. coli. The difference of total bacteria was tested using independent sample t-test. Mean of total fecal LAB of children in KP was 7.58±0.61 CFU/g, whereas in WL was 7.19±0.83 CFU/g. There was no significant difference between the number of fecal LAB of children in KP and in WL (p = 0.255). Mean of total fecal E. coli of children in KP was 7.06±0.75 CFU/g. whereas in WL was 7.23±1.12 CFU/g. There was no significant difference between the number of fecal E. coli of children in KP and in WL (p = 0.078). Further research is needed to determine the factors that can affect the composition of the gut microbiota.
  Harry Freitag Luglio , Fatma Zuhrotun` Nisa , Mirza Hapsari Sakti Titis Penggalih , Siti Helmyati , Lily Arsanti , Siti Budi Utami , Intan Esa Putri , Mutiara Tirta , Susetyowati , Emy Huriyati and Toto Sudargo
  Background: Socioeconomic status (SES) has an important role in defining trend of obesity and it is believed that rich people of poor countries who live in urban areas show high rate of obesity. However, there is a controversy regarding the role of socioeconomic factors on overweight and obesity of people living in rural areas. Objectives: The aim of this study was to analyze the effect of socioeconomic factors on the prevalence of obesity in women living in Raas and Sapudi islands that represents Indonesian rural areas. Materials and Methods: A total of 376 housewives, living in remote Indonesian islands (Raas and Sapudi), took part in this cross sectional study. In this study, nutritional status measurements, interviews and observations done by trained enumerators. A student’s t-test was used to compare differences of socioeconomic factors among women with and without overweight/obesity. Results: Distance to the food source and expenses to buy food were related to overweight/obesity (p<0.05). Additionally, overweight/obese women were married at a younger age compared to those women who were not overweight (16±3 yo vs. 17±6 yo, p = 0.008). Economic status, such as monthly income and possession of the farm or ranch was not associated with overweight/obesity. However, women who work outside home were less likely to become overweight/obese (OR: 0.321; p = 0.001). Conclusion: The prevalence of obesity in women living in those remote islands was at an alarming rate and socioeconomic factors also have an important role in the prevalence of obesity.
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