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Articles by Siti Khalijah Daud
Total Records ( 2 ) for Siti Khalijah Daud
  Sobri Hussein , Rusli Ibrahim , Anna Ling Pick Kiong , Nor`aini Mohd Fadzillah and Siti Khalijah Daud
  The first successful micropropagation protocol for an important recalcitrant medicinal plant in Southeast Asia, Eurycoma longifolia Jack or Tongkat Ali as known in Malaysia was developed. In attempts to establish the somatic embryos of E. longifolia, the potential of cotyledon, zygotic embryo, leaf, petiole, stem and taproot in forming embryogenic callus were examined in the basal Murashige and Skoog (MS) medium supplemented with different auxins at various concentrations. Only cotyledon explants were able to form embryogenic callus in the presence of 1.0 mg L -1 (w/v) of 2,4-dichlorophenoxyacetic (2,4-D). A higher yield (60%) of embryogenic callus was obtained when the Type 4 method dissected cotyledon explants were cultured in basal MS medium containing 0.5 mg L -1 (w/v) of kinetin and 1.0 mg L -1 (w/v) of 2,4-D. The highest number of somatic embryos (45±2) was observed in the same medium formulation with the addition of 1.0 g L -1 (w/v) activated charcoal. Subsequent transfer of these mature somatic embryos in basal MS media supplemented with 1.0 mg L -1 (w/v) of kinetin produced a 90% of plantlet regeneration. The differences between the embryogenic and non-embryogenic callus were also determined based on the histological studies.
  Anna Ling Pick Kiong , Maziah Mahmood , Nor’aini Mohd Fadzillah and Siti Khalijah Daud
  Production of the four targeted triterpenes, asiatic acid, madecassic acid, asiaticoside and madecassoside in leaf derived callus and cell suspension cultures of Centella asiatica was compared with its production in whole plant from field and in vitro shoot cultures. In callus a cultures, production was found the highest during the third week of culture and the contents declined gradually. The glycosides, madecassoside and asiaticoside contents were found higher than the asiatic acid and madecassic acid in callus cultures. Four triterpenes precursors, squalene, Farnesyl Pyrophosphate (FPP), Isopentenyl Pyrophosphate (IPP) and leucine, which are involved directly or indirectly in the triterpene biosynthetic pathway, were used to increase the triterpenes production. Squalene was found the best precursor in promoting triterpenes production in callus cultures. Squalene treated callus also produced the highest biomass production compared to the other precursors tested.
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