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Articles by Siti Khairani Bejo
Total Records ( 3 ) for Siti Khairani Bejo
  Yaqub Ahmed Geidam , Zunita Zakaria , Saleha Abdul Aziz , Siti Khairani Bejo , Jalila Abu and Sharina Omar
  Prevalence of multidrug resistant bacteria in apparently healthy chickens from 3 selected poultry farms in Selangor area of Malaysia was investigated. Conventional isolation techniques such as growth on selective media, gram staining and biochemical tests were utilised for the identification of the different bacterial isolates. Antimicrobial sensitivity test was monitored with the disc diffusion assay against 12 antimicrobial agents. A total of 96 Staphylococcus aureus, 48 E. coli, 7 Pasteurella sp. and 6 Salmonella sp. were isolated. All E. coli and Salmonella spp. isolates were multidrug resistant while 77.2% of Staphylococcus aureus and 71.5% of Pasteurella sp. isolates were multidrug resistant. The study further revealed highest resistance to tetracycline while cephalothin as the best drug of choice for treatment of infections caused by the isolates in the study area. Since not only chickens are at risk, this study recommends urgent intervention by regulatory agencies to limit the emergence and spread of these bacteria as well as prudent use of antibacterial agents among farmers in Malaysia.
  Ali Abuseliana , Hassan Daud , Saleha Abdul Aziz , Siti Khairani Bejo and Milud Alsaid
  Streptococcal infection was reported to cause significant mortality and high economical loss in freshwater and saltwater fish species including tilapia species, worldwide. Recently, few disease outbreaks affecting cultured red tilapia in Selangor state, Malaysia was investigated. Affected fish showed loss of appetite, serpentine swimming and exophthalmia. Sick and healthy appeared fish were clinically examined and samples from brain, liver, spleen and kidney were collected for agent isolation. All isolates were gram-positive, oxidase-negative, catalase-negative, β-haemolytic cocci and were characterized as a Group B Streptococcus agalactiae (GBS) using commercial identification kits (Streptococcal grouping Kit, RapID™ STR System and BBL Crystal GP ID Kit). The isolates were sensitive to amoxicillin, ampicillin, erythromycin, chloramphenicol, linomycin, rifampicin, vancomycin, gentamicin, sulfamethoxazole + trimethoprime and tetracycline. In contrast, they were resistant to neomycin, amikacin, kanamycin and streptomycin. Specific Polymerase Chain Reaction (PCR) and 16S rRNA sequencing technique results confirmed the isolates as a GBS. Results of this current study indicated that the Streptococcus agalactiae infection started to spread and warrants focusing on ways to prevent and control the disease before it become endemic.
  Ali Farag Abuseliana , Hassan Hj Mohd Daud , Saleha Abdul Aziz , Siti Khairani Bejo and Milud Alsaid
  The rapid increases in global aquaculture industry have exposed many diseases that were not known in aquaculture fields. Streptococcus agalactiae, one of Streptococci species that infecting both terrestrial and aquatic animals. The organisms have been isolated from numerous fish species in natural disease outbreaks and showed to be pathogenic to several fish species. Recently, few streptococcosis outbreaks affecting cultured Red tilapia in Selangor were investigated and the S. agalactiae isolate was used to study its pathogenicity on juvenile Red tilapia. The 120 h 50% endpoint (LD50) value in juvenile tilapia injected Intraperitoneally (IP) was 1.56x105 cfu mL-1. Experimental infections were carried out by bathing the fish for 30 min in water containing the bacteria and by IP injection. It was observed that IP route was more potent to cause mortality to juvenile Red tilapia and produced clear clinical signs within 5 days. It was noted that the mortality started to reduce after 5 days and fish recovered after 9 days post inoculation. In contrast, immersion route did not induce mortality but produced moderate clinical signs such as lethargy and loss of appetite and fish started to recover after 6 days. The findings of the current study would enable us to formulate a suitable measure to prevent and control future disease outbreaks.
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