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Articles by Siti Isrina Oktavia Salasia
Total Records ( 4 ) for Siti Isrina Oktavia Salasia
  Desy Cahya Widianingrum , Sarasati Windria and Siti Isrina Oktavia Salasia
  Antimicrobial resistance patterns and gene encoding for methicillin/oxacillin resistance (mecA) were determined in 73 Staphylococcus aureus. The isolates of S. aureus originated from bovine (39 isolates) from Peranakan Etawa (PE) or crossbred Etawa goats (24 isolates) and from patients of Sarjito hospital (10 isolates) in Indonesia. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene and thermonuclease (nuc) genes. Staphylococcus aureus originating from human and bovine were more resistant than those of goat origin. Seventeen S. aureus (23.29%) were resistant to single antibiotic and 15 isolates (20.55%) showed resistance to two antimicrobial agents. Multi resistances were found in 26 (35.62%) of S. aureus isolates. Resistance to ampicillin was the most common finding (80, 76.92 and 41.67%), followed by gentamicin (30, 51.28 and 25%), oxacillin (50, 38.46 and 58.33%), tetracyclin (40, 28.21 and 16.67%) and erythromycin (40, 23.08 and 20.83%) for human, bovine and goat, respectively. By PCR amplification could be observed in 5 (12.82%) methicillin/oxacillin resistant (mecA) genes for bovine isolates, 1 (4.17%) goat isolates and 9 (90%) human isolates. These isolates were identified as methicillin resistant S. aureus (MRSA). Most of MRSA were resistant to oxacillin (60%), ampicillin (66.7%), tetracyclin (40%), erythromycin (33.3%) and gentamicin (20%) The resistancy of S. aureus to methicillin/oxacillin and the other antibiotics in the present study might help to understand the distribution of methicillin resistant S. aureus (MRSA) among human bovine and goat isolates and might help to control S. aureus infections.
  Ocky Karna Radjasa , Siti Isrina Oktavia Salasia , Agus Sabdono , Jutta Weise , Johannes F. Imhoff , Christop Lammler and Michael J. Risk
  A marine bacterium associated with soft coral Sinularia polydactyla collected from Bandengan water, Jepara, North Java Sea, Indonesia, was successfully screened for antibacterial activity against pathogenic bacterium Streptococcus equi subsp. zooepidemicus K6.72 isolated from infected monkey of the island of Bali and identified based on morphological, biochemical and molecular methods. Marine bacterium was identified as Pseudomonas sp. based on its 16S rDNA and was found to amplify gene fragments of Non-ribosomal peptide synthetase (NRPS). Cloning and subsequent sequencing, a 360 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high similarity of 61.1% to genes peptide synthetase of Bacillus subtilis.
  Sarasati Windria , Desy Cahya Widianingrum and Siti Isrina Oktavia Salasia
  This study aimed to identify staphylococcal mastitis milk in Peranakan Etawa (PE) or Etawa crossbred goats in Indonesia. Total of 93 milk samples used in this study, there were 49 (52.7%) subclinical and 3 (3.2%) clinical mastitis samples. From 52 subclinical and clinical mastitis samples could be isolated 20 (38.5%) staphylococci. Phenotypic identification were performed based on the Gram staining, Mannitol Salt Agar (MSA), catalase, Voges-Proskauer (VP) and coagulase tests. Molecular identification were determined using the Polymerase Chain Reaction (PCR) to detect several genes such as 23S rRNA, nuc and coa genes and confirmed with DNA sequencing. Based on phenotypic and genotypic identification, could be identified 10 Coagulase Positive Staphylococci (CPS) indicated Staphylococcus aureus and 10 Coagulase Negative Staphylococci (CNS) isolates, including Staphylococcus pasteuri (3 isolates), Staphylococcus xylosus (5 isolates) and Staphylococcus haemolyticus (2 isolates). From 3 clinical mastitis of PE goats in our study could be isolated three different staphylococci i.e., S. aureus, S. pasteuri and S. xylosus. This finding indicated that both CPS and CNS have important role as causative pathogen of mastitis in PE goats.
  Novra Arya Sandi and Siti Isrina Oktavia Salasia
  Honey is natural food that common used directly by the society. Forest honey produced by wild bees (Apis dorsata) while the other type of honey produced by Apis mellifera. Beekeeping A. mellifera with monoflora or multiflora nectar is generally carried out by various countries including Indonesia. Scientifically, honey containing bioactive compounds with antimicrobial properties but still uncertain which compounds that play a role in these activities. Reported bioactive compounds that have antibacterial activity of honey are inhibine and non-inhibine. Inhibine is forming enzyme and accumulation of hydrogen peroxide (H2O2) to dilute honey and nectar. The H2O2 has also long been known as an effective antibacterial and major component of penicillin, especially notatin. The types of antibacterial compounds influenced by nectar-source plants with compounds including alkaloids, flavonoids and glycosides. Some researchers reported that beside phytochemicals, antibacterial activity due to presence of Lactic Acid Bacteria (LAB) that produce bioactive compounds with antibiotic-like activity. Some studies reported 3 LAB strains potential to produce bioactive compounds with activity resembles antibiotics, namely L. kunkeei Fhon2, L. kunkeei Lahm and L. kunkeei Yubipro with the greatest inhibition zones than other Lactobacillus. Honeybees are isolated from the stomach LAB capable of producing organic acids, free fatty acids, ethanol, benzoic acid, enzymes, H2O2 and antimicrobial peptides. Different character of the bioactive compounds will jointly deliver results on inhibition zone and broad spectrum for various types of microbial pathogens. Microbial pathogens tested including Serratia marcescens, Klebsiella aerogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and methicilin resistant Staphylococcus aureus (MRSA).
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