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Articles by Shijun Li
Total Records ( 4 ) for Shijun Li
  Shijun Li , Zhenhua Liang , Cui Wang , Yanping Feng , Xiuli Peng and Yanzhang Gong
  In the present study, we investigated the effect of squalene on the reproductive performance in meat-type male chicken. A total of 480 meat-type males, which were 72 weeks old with body weight of 6.0±0.5 kg were assigned randomly and equally to 4 groups in two independental experiments which represent two classic mating types AI (Artificial Insemination) and NM (Natural Mating), respectively. Each experiment contains two groups, control group and squalene-treated group in which meat-type males were fed with the supplemental squalene at a daily dose 10 mg/kg/day for 45 days. Semen data such as average collection semen volume, sperm count and egg fertile rate were collected from experiment I that was designed keeping males and females seperated and using artificial insemination as mating type. In the experiment I that designed keeping birds together in floor and using natural mating as mating type, only fertility data were collected. Also, serum testosterone levels were measured in squalene-treated, control and males no ejaculation groups. In the experiment I, the average collection semen volume is 393 μL, which is significantly higher (p<0.001) than the control group, which is 287 μL. The egg fertile rate has no significant difference between these two groups. In experiment II, the egg fertile rate of squalene treated group was significantly higher than control group (p<0.001).The serum testosterone levels of squalene treated group was significantly higher (p<0.05) than control group. The results showed that meat-type males treated with squalene increased the serum testosterone level and semen collection volume and did not decrease egg fertile rate in AI model and also increased the egg fertile rate in natural mating model. These findings have implications that squalene has a prompt effect on reproductive performance on meat-type male chicken.
  Zhenhua Liang , Cui Wang , Huawen Yu , Xiuli Peng , Yanping Feng , Yanzhang Gong and Shijun Li
  The tyrosinase family is known to be crucial in the melanin biosynthetic pathway and is responsible for the rate limiting step. In the present study, the complementary DNA (cDNA) of TYP1 was cloned from the eye of duck by homology cloning and rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of TYP1 consisted of 2123 nucleotides, containing an Open Reading Frame (ORF) of 1608 bp that encoding a 536 amino-acid peptide, a 5'-terminal Untranslated Region (UTR) of 255 bp and a 3'-terminal UTR of 260 bp with two canonical polyadenylation signal sequence (AATAAA) and a poly(A) tail. The phylogenetic tree display that TYP1 protein is highly conserved and the deduced peptide shares 70.9-93.7% similarity with quail, chicken and mammalian TYP1 proteins. The Semiquantitative RT-PCR analysis indicated that the transcripts of TYP1 mRNA had the highest expression in eyes and black hair follicle, intermediate in white hair follicle and negligible or absent in skin, muscle, heart, liver, kidneys, spleen, lungs, brain and intestine. The study may useful for the further study on polymorphism and correlation on duck feather color and the function of TYP1 of birds.
  Shijun Li , Wenhua Yu , Cui Wang , Zhenhua Liang , Xiuli Peng , Yanping Feng and Yanzhang Gong
  Stem Cell Factor (SCF) also called Steel Factor (SF), Mast cell Growth Factor (MGF) and KIT tyrosine Kinase receptor Ligand (KL) is a hematopoietic growth factor and ligand for the KIT tyrosine kinase receptor. In this study, the partial complementary DNA (cDNA) of SCF was cloned from the eye of duck by homology cloning and Rapid Amplification of Cdna Ends (RACE) approaches. Comparing with zebra finch, quail, chicken and mammalian SCF cDNA sequence, the phylogenetic tree displayed that duck SCF is highly conserved, it shares 63.8-94.3% similarity with the above species cDNA sequence. The rooted phylogenetic neighbor-Joining tree with bootstrap was done using SCF CDS sequences from duck and other species by DNAMAN software. The results showed that duck has close relationship with chicken, quail and zebro finch. The semi-quantitative RT-PCR analysis indicated that SCF was a universally expressed gene, it was detected in heart, liver, spleen, kidney, brain, back skin, muscle, eye, glandular stomach, belly skin. Q-PCR was employed to analyze the SCF gene expression in six type duck hair bulbs. The results showed that its expression had significant difference between W-W which is white hair bulb taken from white plumage with yellow beak and other types (BL-Bl which is black feather hair bulb taken from black plumage with black beak, W-L which is white feather hair bulb taken from white plumage with black beak ducks, W-WB which is white feather taken from white-black plumage with black beak, B-WB which is black feather taken from white-black plumage with black beak), p<0.001). The expression of SCF in BR (brown feather hair bulb taken from brown plumages with black beak ducks) has significant difference comparing with the above 5 hair bulb types (p<0.05). There is no significant difference between any of the following types which including BL-BL, W-L, W-WB and B-WB. The results indicated that SCF may be a critical gene on regulation gene expression for duck plumage diversity.
  Yongping Yang , Ping Gong , Shijun Li , Xiuli Peng , Yanping Feng and Yanzhang Gong
  Lipoprotein Lipase (LPL) is a multifunctional protein that plays a major role in the hydrolysis of triglycerides present in chylomicrons and very low-density lipoproteins. This study was designed to investigate the effects of Single Nucleotide Polymorphisms (SNPs) of the duck LPL gene on fatness and carcass and growth traits. A White Kaiya x White Liancheng F2 population with a total of 1069 individuals was used in the present study, 440 healthy ducks randomly selected from this population were slaughtered at 80 days of age. PCR-Single Strand Conformation Polymorphism (PCR-SSCP) and sequencing methods were used to detect SNPs in the duck LPL gene. Two new SNPs (C645T and G726A) were discovered in exons 5 and genotyped using the PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Method. Genotyping results showed that the genotype distribution differed between males and females, thus the association analyses were performed separately for males and females. Association analyses indicated that the SNPs were significantly associated with body weight, fatness and carcass traits (p<0.05 or p<0.01). We concluded that LPL is a major gene or is linked with a major gene that influences body weight, fatness and carcass traits and that C645T and G726A could be used as candidate molecular genetic markers for breeding selection.
 
 
 
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