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Articles by Sherief I. Khalifa
Total Records ( 2 ) for Sherief I. Khalifa
  Naglaa M. Mohamed , Russell T. Hill , Raouf W. Kilada , Sherief I. Khalifa and Soad H. Abou-El-Ela
  Morphologically, two species of giant clams Tridacna have been recognized in the Red Sea, T. squamosa and T. maxima. Similar morphology among species makes classification difficult. For proper classification, we sequenced an approximately 450-nucleotide fragment of the mitochondrial 16S rDNA gene from the tissues of Tridacna from eight locations in the Red Sea. Our results suggest that there are three novel species of Tridacna in the Red Sea. Two of these species are related to T. maxima and one is related to T. squamosa. Of the two species related to T. maxima, one species was found in Hurghada, Marsa Ghaleb and Safaga while the other was found in Abu Zenima, Abou Galum, Dahab and Nuweiba. All three novel species were found in Ras Mohamed. Our results do not support the morphological classification that suggests the existence of only two tridacnid species, T. maxima and T. squamosa in the Red Sea.
  Shymaa E. Bilasy , Sherief I. Khalifa , Hesham Saeed , Samy M. Saleh and Soad H. Abou El-Ela
  Sarcophyton species are soft corals abundant in the Red Sea. Sarcophyton glaucum produces sarcophine that has a well documented antitumor activity and inhibitory activity against tumor promoters. This study was established to test the accuracy of the morphological and sclerite based taxonomy against the chemical fingerprints and the protein profiles for the same samples. High performance thin layer chromatography and high performance liquid chromatography analyses were performed on the organic extract obtained from the samples. The analyses revealed that all Sarcophyton samples belonging to different species contained sarcophine with varying concentrations. Total cellular proteins were extracted and subjected to 10% Sodium dodecyl sulphate polyacrylamide gel electrophoresis. Characteristic protein profiles were obtained for the different species studied. The data obtained from the protein profiles and the chemical fingerprints were consistent and in some instances disagreed with the morphological and/or the sclerite based identification of the Sarcophyton species. Furthermore, one of the Sarcophyton samples from Sharm El-Sheikh could not be identified by sclerite or morphological methods. It gave chemical and protein profiles that were different from all the other samples in this study.
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