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Articles by Sheikh Riazuddin
Total Records ( 6 ) for Sheikh Riazuddin
  Shahid Karim , Muhammad Murtaza and Sheikh Riazuddin
  A large number of Bacillus thuringiensis (Bt) isolates separated from samples collected from different economic environments in Pakistan were characterized for Crystal Protein gene (cry) composition and pesticidal activity against devastating polyphagous pest, Helicoverpa armigera. Isolates harboring combinations cry2A genes were found me efficacious against the target pest. One isolate designated as PR17.4 (CEMB Bt) exhibited high levels of toxicity again H. armigera in lab biotoxicity assays and was therefore, chosen as a potential candidate in integrated Pest Management (1PM) strategies to control this notorious pest of valuable crops. Field efficacy of home grown cells CEMB Bt, PR17.4, Agr (50 WP), was compared with a commercial BT formulation of Novartis, growth regulators, Match (50 EC), Insegar (25 W and a popular chemical pesticide, Curacron (500 EC) to investigate their effectiveness to control H. armigera in potato crop Insegar was not significantly effective against the target pest. Locally isolated CEMB Bt was found to be as effective the commercial Bt and chemical control agent of Novartis. A synergistic effect was also observed among, Match/Agree CEMB Bt/Match, Match/Curacron and Agree/Curacron combinations. These studies suggest that CEMB Bt alone or combination with other biological or chemical based pesticides can be safely recommended for pest management strategy against H. armigera with no obvious harmful effects on its predators as is the case with chemical insecticides.
  Muhammad Idrees , Aftab H. Bhatti , Sheikh Riazuddin and F.M. Khan
  A total of 120 patients with positive cultures were tested with eight commonly used anti-tuberculosis drugs i.e. Isoniazid (H), streptomycin (S), Para aminosalicylic acid (PAS), Ethionarnide (ETH), Ethambutol (E), Thiacitazon (THI), Rifampicin and Pyrazinamide (Z). Standard proportion method as recommended by CDC Atlanta, Georgia (USA) using L-J medium was adopted. The isolates of M. tuberculosis were identified by their acid-fast character on Z-N staining, clonial growth on medium, niacin accumulation, nitrate reduction, positive catalase test and amplification of a 541-bp fragment from IS986 gene by Polymerase Chain Reaction (PCR). Sixty one (50.83%) of the isolates were resistant to one or more tuberculosis drugs. Of these resistant patients, 10 (16.39)%) were resistant to only one anti tuberculosis drug while (83.6%) were resistant to more than one drug. As regards resistance to individual drugs, 33 (27.5%) of the isolates were resistant to H, 22 (18.33%) to S, 42 (35%) to PAS, 21 (17:5%) to ETH, 20.0 (16.67%) to E, 8 (6.67%) to THI, 24 (20.0 to R and 34 (28.34%) to Z. These findings showed a growing concern of increasing prevalence of drug resistan tuberculosis.
  Ahmad Usman Zafar , Shahid Karim , Idrees Ahmad Nasir and Sheikh Riazuddin
  Previous lab report (Karim et al., 1999) had shown the efficacy of CAMB Bacillus thuringiensis preparation in potato field to control Helicoverpa armigera. In present study, CAMB Bt formulations based on locally isolated Bt strain were checked against 1st to 4th instar larvae of H. armigera by incorporating Bt proteins into artificial diet. Early instars showed significant susceptibility to Bt formulation. Laboratory assays of stored CAMB Bt formulations did not show any significant change in their toxicity towards H. armigera larvae in laboratory assays. Potency of stored Bt formulations for 12 and 24 months were also tested in small scale experiment conducted under contained conditions on tomato crop. All formulations were found effective to control H. armigera larvae in tomato crop. The present study shows that CAMB Bt formulation can be used in field applications to control target pests after long storage time.
  Shahid Karim , Ahmad Usman Zafar , Idrees Ahmad Nasir and Sheikh Riazuddin
  CAMB Bacillus thuringiensis formulation and two other commercial Bt formulations (Agree and Larvo Bt) were tested on Okra fields to control two lepidopteran pests, Helicoverpa armigera and Earias vitella. CAMB Bt formulation was tested from 250-g/h dose to 1500 g/h dose to see the efficacy against target insect pests. Commercial Bt formulations, Agree and Larvo Bt were used as standard with one dose of 1000 g/h. All microbial insecticides successfully controlled H. armigera and E. vitella larvae in okra field. The efficacy of locally developed Bt formulation was promising in comparison to Agree and Larvo BT.
  Tauqeer Ahmad , Muhammad Saeed Ahmad , Idrees Ahmad Nasir and Sheikh Riazuddin
  The ability of explants of gladiolus cv White Prosperity to form plantlets and cormlets was investigated in vitro studies. Maximum production of shoots per explant was obtained on basal medium of Murashige and Skoog (1962) containing 18 μM 6-Benzylaminopurin (BAP) and 36 μM Indole-3-butyric Acid (IBA). Callus formation was observed when in vitro cultures were maintained for 16-18 weeks. When different explants were used for their response to regenerate, maximum number of shoots per culture was achieved from nodal buds. In order to allow initiation of root formation, the shoots were transferred to one half strength Murashige and Skoog medium containing 2.5 μM, Naphthalenacetic Acid (NAA) or 19 μM Indole -3-butyric Acid (IBA). Roots developed readily within 4-5 weeks on either of the two media. However, all plantlets grew with better survival in medium containing NAA as harmonal supplement than in media containing IBA. Further MS medium containing 6 percent sucrose facilitated maximum growth of cormlets to allow their use as propagation material in vivo.
  Samina Noor , Tayyab Husnain and Sheikh Riazuddin
  A simple leaf tip assay was used for screening of putative transgenic plants expressing the hygromycin resistance gene (hph) or kanamycin resistance gene (nptll). Leaf tips were excised from in vitro or in vivo transgenic plants and cultured on MS medium without phytohormons containing the suitable concentrations of hygromycin and kanamycin. Leaf tips of plants transformed with the marker gene showed no significant effect of the appropriate drug for at least two weeks. While non-transgenic leaf tips had noticeable symptoms of bleaching, necrosis or browning after 3-4 days of selection. This method is simple, rapid and allows clear distinction between transformed and non-transformed plants both, in monocots and dicots.
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