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Articles by Sheikh Riazuddin
Total Records ( 6 ) for Sheikh Riazuddin
  Ahmad Ali Shahid , Idrees Ahmad Nasir , Ahmad Usman Zafar , Aleena Sumrin , Bushra Chaudhry and Sheikh Riazuddin
  The biopesticides based on fungus Metarhizium anisopliae and bacteria Bacillus thringiensis (Bt) were used on stem borer and leaf folder of rice, which have reduced the population of these pests effectively both in laboratory and in the field. In laboratory bioassays CAMB biopesticides were very useful on insect larvae of Helicoverpa armigera. By using the combination of fungal and bacterial formulation the increase in mortality was studied and it was 100% after 96 h. In the field trials a significant effect on stem borer and leaf folder was observed. On the basis of these results, biopesticides can safely be recommended for the control of rice pests with no harmful effect on its predators as in case with chemical pesticides.
  Tayyab Husnain , Tahira Fatima , Rafi-ul-Islam and Sheikh Riazuddin
  In order to develop an efficient protocol for inserting novel genes in chickpea plants, conditions of regeneration and transformation of tissues were optimized. In case of in vitro plant regeneration of Cicer arietinum variety 6153, the hypocotyl tissue showed somatic embryogenesis and regenerated shoots in two basal media with addition of NAA and BAP. Different auxins seemed to have different effects on the differentiation and maturation of embryos. The replacement of one auxin NAA with another IAA induced the germination of embryos. Elaborate rooting of regenerated shoots in two basal media containing IBA was obtained with a frequency of 80 percent. Conditions of biolistic transformation were also optimized for transient expression of marker gene using a homemade particle acceleration gun. The beta-glucuronidase gene was introduced into hypocotyl tissue for transient expression and upto 58 percent of hypocotyl showed transient expression. These results provide the basic information for the transformation of chickpea via somatic embryogenesis.
  Tahira Fatima , Asad Jan , Tayyab Husnain and Sheikh Riazuddin
  Efforts were made to optimize tissue culture conditions for quick establishment of cell suspension and simple procedure for the cryopreservation of embryogenic cells suspension cultures of three rice varieties. Suspension cultures were initiated from friable, globular embryogenic calli in MS/R2 media supplemented with 2mg/l 2,4-D. These suspension cultures were regenerated and cryopreserved. Both MS and R2 media supplemented with 2mg/l 2,4-D were quiet suitable for developing cell suspension. All the three varieties gave compact light yellow calli on MMS medium. The regeneration frequency was 55% on average for 10-14 weeks old cell suspension. Vigorous regeneration was observed on MS containing sorbitol. Post thaw cell viability varied from variety to variety. No significant difference in viability was found in varieties cryopreserved for different periods. In conclusion cryopreservation can be used to preserve cell suspension line for a reasonable time. It can easily be used on culture medium or liquid R2 medium.
  Khurram Bashir , Muhammad Rafiq , Tahira Fatima , Tayyab Husnain and Sheikh Riazuddin
  In present studies, level of toxicity of hygromycin was optimized for local inbred line of maize (Zea mays L.). The immature embryos were isolated and cultured on N6 media modified with 1.0 mg L-1 2,4-D, 25 mM L-Proline and 100 mg L-1 Casein Hydrolysate and containing different concentrations of hygromycin. These immature embryos were observed for callus formation on working concentrations of 0, 20, 40, 60, 80, 100 and 120 mg L-1 hygromycin. The results showed that 97.3 38.5 and 32.5% calli survived on media containing 0, 20 and 40 mg L-1 hygromycin. Hygromycin concentration of 60 mg L-1 and above was effective for BR-6 as no callus formation was observed at this concentration or the immature embryos were completely dead. It was also observed that although 32.8% calli survived on media containing 40 mg L-1 hygromycin, these calli were slower in growth, smaller in size and pale in color as compared to calli containing (hph) gene on same media. On the basis of these results 40 mg L-1 hygromycin seems suitable for identification of putative transformants.
  Rozina M. Ali , Tayyab Husnain , Syed Sarfraz Hussain , Nasir Mahmood and Sheikh Riazuddin
  Induction of multiple shoots in Gossypium hirsutum L. variety CIM-443 has been achieved by using meristem and cotyledonary node as explants. Meristems of size 3-4 mm were excised from embryos isolated from seeds while cotyledonary nodes were cut from seven days old seedling and the mean shoot number per explant response for total of three experiments was maximum i.e., (6.72±0.79) and (4.92±0.67), respectively for both explants in MS medium supplemented with benzyl-amino purine (BAP) 1.0 mg L-1 + naphthalene acetic acid (NAA) 0.05 mg L-1. Shoot elongation was observed in MS medium amended with NAA 0.1 mg L-1. The percentage of shoots forming roots was maximum (79.16) in case of 1/2 MS supplemented with NAA 0.05 mg L-1. Rooted plantlets hardened in soil and normal boll formation observed.
  Ahmad Usman Zafar , Idrees Ahmad Nasir , Ahmed Ali Shahid , Muhammad Sarwar Rahi and Sheikh Riazuddin
  CAMB Bt. based and fungus based biopesticides, commercial Bt. formulation from mycogen and a new chemical pesticide Methoxyfenozide (RH2485-240SC) were tested on cauliflower field against cabbage butterfly (Pieris brassicae). All pesticides successfully controlled the population of cabbage butterfly in cauliflower crop. The efficacy against I to V instar larvae and field stability of CAMB Bt. biopesticide was better than chemical and other biopesticides. So, CAMB Bt. can be safely recommended for pest management strategies against Lepidopteral pests on vegetables with no harmful effects on its predators as in case with chemical pesticides.
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