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Articles by Shaymaa Fadhel Abbas Albaayit
Total Records ( 2 ) for Shaymaa Fadhel Abbas Albaayit
  Shaymaa Fadhel Abbas Albaayit , Ahmed S.K. Al-Khafaji and Hala M. Radif
  Background and Objective: In recent years, there has been an increasing interest in discovering the role of plasmids in emerging fluoroquinolones resistance implicated in serious difficulties of nosocomial infections’ therapy. Therefore, this study aimed to explore the association of Pseudomonas aeruginosa plasmid DNA in emerging fluoroquinolone resistance. Materials and Methods: Thirty P. aeruginosa isolates were diagnosed in 45 specimens collected from patients with infected burns who attended the local hospitals in Baghdad. Antibiotic susceptibility of the studied isolates to different fluoroquinolones (ciprofloxacin, levofloxacin, norfloxacin and ofloxacin) has been investigated and showed variable responses. Plasmid DNA profiles of P. aeruginosa cells were also investigated utilizing QIAprep Spin Miniprep Kit and distinguished by agarose gel electrophoresis. In order to investigate the association between the fluoroquinolone resistant isolates and their content of plasmid DNA, two strategies were adopted: (1) SDS-based plasmid curing technique and (2) Bacterial transformation by plasmid DNA. Results: The results illustrate that the plasmid elimination from P. aeruginosa progeny cells has slightly increased fluoroquinolones response in comparison to the parental cells and Ciprofloxacin was the more susceptible antibiotic to P. aeroginosa. Further evidence was obtained from transformation of TOP10 Escherichia coli by P. aeruginosa extracted plasmids. Transformed E. coli cells exhibited resistance to some of the fluoroquinolones (Ciprofloxacin). Conclusion: The findings suggested that P. aeruginosa plasmid content could be a preliminary determinant for fluoroquinolone-based therapeutic regimens of nosocomial infections caused by P. aeruginosa.
  Shaymaa Fadhel Abbas Albaayit and Rukesh Maharjan
  Background and Objective: Zerumbone has been reported to exert anti-inflammatory, anti-ulcer and anti-hyperglycemic effects but the specific mechanism through which zerumbone exerts its anti-inflammatory action through inhibiting reactive oxygen species was not well studied. Hence, this paper studied the zerumbone capacity to inhibit intracellular and extracellular Reactive Oxygen Species (ROS) produced by whole blood cell, polymorphoneutrophil (PMNs) and macrophage cells due to the zymogen and phorbolmyristerate acetate (PMA) oxidant effect. Materials and Methods: Zymogen and PMA based chemiluminescence assay were used to determine the immunomodulatory effect of zerumbone at concentrations (100, 10 and 1 μg mL1) toward production of Reactive Oxygen Species (ROS) from whole blood, PMNs and macrophage. Results: Zerumbone significantly inhibited intracellular and extracellular ROS production by the zymosan/PMA-activated phagocyte cells with IC50 values of (16.3±0.1, 23.7±0.1 and 4.97±0.1 μg mL1) against whole blood, PMNs and macrophage respectively. Conclusion: The anti-inflammatory activity of zerumbone was so much significant that even strong oxidant (zymogen and PMA) were not able to produce reactive oxygen species when incubated together in phagocytic cells, thus suppress production of ROS. Therefore, it is highly used in herbal medicine as a potent immunomodulatory therapy in various inflammation associated diseases.
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