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Articles by Sharmeen Rahman
Total Records ( 2 ) for Sharmeen Rahman
  Sharmeen Rahman , Daniel Schmidt and Jane Hughes
  Background and Objective: High quality genomic DNA is essential for any Genotyping by sequencing technique (e.g., Restriction Site Associated DNA Sequencing). However, producing high quality genomic DNA from crustacean specimens has been difficult due to rapid degradation of tissue samples. In this study, an effective preservation and subsequent DNA extraction procedure was described for producing high quality genomic DNA in a crustacean shrimp, Paratya australiensis. Materials and Methods: Tissue samples were preserved following three preservation techniques: freezing (-80°C), 100% ethanol and RNAlater. Then DNA was extracted from each type of preserved sample using three different methods namely Econo spin column extraction, CTAB and salt extraction. Results: High quality DNA was produced only through Econo spin column extraction method from tissue samples preserved in 100% ethanol and RNAlater. In this case DNA fragments of >10,000 bp were produced without any smear or sign of degradation. The CTAB and salt extraction methods demonstrated very low quality or degraded DNA for all other types of preserved samples which is unusable for Restriction Site Associated DNA Sequencing (RAD-seq) library preparation. Conclusion: High quality genomic DNA was extracted only through Econo spin column extraction process preserving sample in 100% ethanol or RNAlater. The approach described here is easy, simple and does not require impractical cryopreservation methods.
  M. Belal Hossain , S.M.N. Amin , M. Asadujjaman and Sharmeen Rahman
  This study was carried out to describe the distribution and community pattern of benthic macrofauna collected from two nationally and internationally important islands, Hatiya and Nijhum Dweep in the period of January to June, 2010 using hand-held mud corer (10x10x10 cm) from seven stations. The coarser (order) level of taxonomic resolution was used to investigate the community attributes among the sampling stations. The study yielded a total of 10688.89 ind. m-2 macrofauna from all stations. Ten major taxa were identified from two islands and polychaete being the dominant. The average population density was 1526.98±1453.375 ind. m-2. There was a significant difference (p<0.05) in faunal density between Hatiya and Nijhum Dweep with later having higher mean density. The maximum (1.97) Shannon diversity index was found in St1 and the lowest (1.14) in St5. The highest similarity (76.6%) was found between St2 and St5. Multivariate analysis was conducted at order level of benthos using PRIMER. Bray-Curtis similarity measures among macrozoobenthic communities separated the stations into several cluster groups which was supported by nMDS ordination map.
 
 
 
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