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Articles by Shaoming Gong
Total Records ( 2 ) for Shaoming Gong
  Cui Wang , Yi Liu , Yunzhou Yang , Huiying Wang , Sifeng Yi , Chuang Li , Shaoming Gong , Weihu Chen and Daqian He
  MyoGenin (MyoG) is a basic Helix-Loop-Helix (bHLH) transcription factor that belongs to the Muscle-specific transcription Factors (MRFs) family which plays critical roles in regulating the skeletal muscle development and growth. In this study, the complete coding sequence and genomic DNA sequence of goose MyoG gene were cloned and characterized. The goose MyoG CDS was composed of 684bp that encoded a 227 amino acid protein, including a highly conserved basic helix-loop-helix domain. Multiple sequence alignments and phylogenetic analysis indicated that the deduced goose MyoG protein was conserved in vertebrates, especially in the avian species. The goose MyoG genomic DNA sequence we obtained was 3444bp and consisted of 3 exons and 2 introns.Sem-quantitative RT-PCR analysis demonstrated that the goose MyoG mRNA was specifically expressed in the breast muscle and leg muscle tissues, little or no expression was observed in heart, liver, spleen, lung, kidney, muscular stomach, brain, intestine and sebum. These data will serve as a foundation for further insight into the functions of the MyoG gene in Chinese domestic goose.
  Cui Wang , Yi Liu , Yunzhou Yang , Huiying Wang , Shaoming Gong , Weihu Chen and Daqian He
  Paired box (Pax) protein 3 is a member of the Pax family of transcription factors, plays critical roles in muscle development. Many studies showed that Pax3 gene was a functional candidate gene for production and meat quality. However, the sequence information of goose Pax3 gene remains unknown. In this study, the comparativegenomicstechnology was used to clone the cDNA sequence of Pax3 gene from the breast muscle tissue of Zhedong goose. The gene structures were analyzed by the bioinformatics software and the mRNA expression profile of Pax3 gene in different tissues was measured by the semi-quantitative RT-PCR. The goose Pax3 full-length coding sequence consisted of 1327 bp and encoded 421 amino acids. Sequence analysis displayed that a splicing variant of goose Pax3 absence of a glutamine residue was also identified. Both of the Pax3-a (inclusion of a glutamine residue) and Pax3-b (exclusion of a glutamine residue) isoforms were predicted contain three conserved domains (a paired domain, an octapeptide region and a homeodomain) and were highly conserved (>90%) relative to the known Pax3 proteins from other species. Multiple sequence alignments and phylogenetic analysis displayed that the deduced goose Pax3 proteins have a close genetic relationship and evolutional distance with the Pax3 proteins in other avian species, especially in ducks. The semi-quantitative RT-PCR results revealed Pax3 mRNA was highly expressed in the breast muscle tissue, followed by the lung, leg muscle and brain, little or no expression was observed in heart, liver, spleen, kidney, muscular stomach, intestine and sebum. These findings will help us understand the functions of the Pax3 gene and the molecular breeding in Chinese domestic goose.
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