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Articles by Shanker Kumar Singh
Total Records ( 3 ) for Shanker Kumar Singh
  Deepti Singh , Amit Kumar Verma , Amit Kumar , Mukesh Srivastava , Shanker Kumar Singh , Arvind Kumar Tripathi , Ashish Srivastava and Iftekhar Ahmed
  The early detection of the Canine Parvo Virus (CPV) is of paramount importance. The present study was aimed to know the molecular epidemiology of Canine parvo virus. Canine faecal samples from 100 dogs showing the clinical signs of gastroenteritis in and around Mathura, Uttar Pradesh, India were collected and DNA was extracted by phenol-chloroform method. CPV vaccine strain was used as a positive control. Polymerase Chain Reaction (PCR) was carried out to amplify VPI/VP2 gene using a set of 20-mer primers [pCPV-RT (Forward): 5’-CAT TGG GCT TAC CAC CAT TT-3’; (Reverse): 5’-CCA ACC TCA GCT GGT CTC AT-3’)] from position 3136-3155 to 3276-3295 of VP1/VP2 gene. A PCR product of approximately 160 bp was generated with positive faecal samples and CPV vaccine strain. After screening, 63 dogs were found positive for CPV but no sex variation was noticed amongst the CPV positive cases. Dogs, of the age group of ≤6 months were more susceptible in comparison to of >6 months and highest occurrence was noted in unvaccinated dogs and dogs in co-habitation with other dogs. Breed wise distribution of CPV in dogs revealed that the prevalence of CPV was the highest in Doberman (77.78%), followed by Spitz (78.57%), German shepherd (70.00%), Labrador (68.75%), Pomeranian (45.45%). It is concluded that CPV is prevalent in the Mathura and nearby area and it is more common in pups of age less than 6 months old and more prevalent in German shepherd, Labrador and Pomeranian breeds of dog.
  Amit Kumar Verma , Amit Kumar , Shanker Kumar Singh , Anu Rahal , Iftekhar Ahmed , Deepti Singh , Aashish Pratap Singh and Lalit Singh
  Globally, Campylobacters have been reported as leading cause of gastroenteritis in man as well as animals and considered as emerging zoonotic problem particularly in developing countries including India. A cross-sectional study was conducted to know the prevalence and epidemiological determinants for Campylobacter spp. in dogs in and around Mathura city, Uttar Pradesh, India. Based on isolation, cultural and biochemical characterization of bacteria, the prevalence of Campylobacter spp. was 34.24%. Younger dogs (less than 1 year of age) were more likely to carry Campylobacter spp. High prevalence of Campylobacter spp. supports the hypothesis that dogs, particularly younger animals, may be an important source of Campylobacter infection for humans. Breed-wise prevalence showed that non-descript dogs (45.97%) were more likely to carry Campylobacter infections. Dogs showing clinical signs of gastroenteritis were showing higher prevalence (47.21%) in comparison to that of animals without gastro-intestinal disorders (15.04%). Out of 113 Campylobacter isolates of canine origin, two isolates were resistant to all the nineteen antibiotics used in the study, while all the isolates were resistant to Streptomycin, Ampicillin, Amoxycillin, Aztreonam, Lincomycin, Tetracyclin, Oxytetracyclin and Penicillin. A high rate of resistance was observed to Cefotaxim (97.35%), Peefloxacin (91.15%), Chloramphenicol (90.27%), Ofloxacin (84.07%), Ciprofloxacin (83.18%), Cefaclor (80.53%), Nitrofurazone (76.11%), Norfloxacin (74.33%), Gentamicin (42.48%), Amikacin (40.71%) and Enrofloxacin (36.28%). Our results indicate Amikacin and Gentamicin as drugs suitable for the treatment of campylobacteriosis in dogs.
  Mahima , Krishna Veer Singh , Amit Kumar Verma , Vinod Kumar , Shanker Kumar Singh and Debashis Roy
  The ‘Hariana’ breed of cattle has been proved to be highly adaptable to harsh climatic conditions and resistant to common diseases prevalent in India. In this study, the normal reference values of hematological and serum proteins and other blood biochemical parameters were determined in the heifers of Hariana breed maintained at Instructional livestock farm complex, DUVASU, Mathura, India. A total of twenty four animals were used in this study. Blood was taken aseptically from all the animals and transported to laboratory for hematological and biochemical analysis. The hematological parameters (Hemoglobin, total erythrocyte count, total leukocyte count, packed cell volume) and biochemical parameters (Total protein, total albumin, albumin globulin ratio, urea, creatinine, calcium, phosphorous, calcium phosphorous ratio, AST, ALT) values were statistically analyzed, mean and standard deviations were calculated and set as reference values. This study reported hematological and serum biochemical values which could serve as baseline information for comparison in conditions of nutrient deficiency, physiological and health status of Hariana cattle heifers in India.
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