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Articles by Shan Sun
Total Records ( 2 ) for Shan Sun
  Shan Sun , Fang Zhang , Shanshan Zhang , Tieling Xing and Shenzhou Lu
  Current surgical dressings and woundplast have drawbacks such as poor permeability, airtightness and biocompatibility, thus a new material is obligatory to replace the traditional ones. Due to outstanding biocompatibility of Silk Fibroin (SF) and excellent antibacterial properties of Dodecyl Trimethyl Ammonium Bromide (DTAB), a cationic surfactant, the SF/DTAB hydrogel formed instantaneously is recommended. By means of SEM, FTIR and XRD, SF/DTAB hydrogel displayed clustered nanofilaments structure and similar secondary structure and morphology with SF solution mainly consisted of random coils in 1 h, then it tended to be the same as pure SF hydrogel which was composed of porous network structure and primarily contained β-sheet structure. Excellent elasticity and antibacterial effect were verified by rheological and antibacterial measurements.
  Shan Sun , Jun-Ping Yu , Feng Chen , Tong-Jin Zhao , Xiao-Hong Fang , Yi-Qin Li and Sen-Fang Sui
  Dehydration-responsive element-binding proteins (DREBs) and ethylene-responsive element (ERE) binding factors are two major subfamilies of the AP2/ethylene-responsive element-binding protein family and play crucial roles in the regulation of abiotic- and biotic-stress responses, respectively. In the present work, we have reported a previously identified DREB-like factor, TINY, that was involved in both abiotic- and biotic-stress signaling pathways. TINY was capable of binding to both DRE and ERE with similar affinity and could activate the expression of reporter genes driven by either of these two elements in tobacco cells. The 15th amino acid in the APETALA2 (AP2)/ethylene-responsive element-binding factor domain was demonstrated to be essential for its specific binding to ERE, whereas the 14th and 19th amino acids were responsible for the binding to DRE. The expression of TINY was greatly activated by drought, cold, ethylene, and slightly by methyl jasmonate. Additionally, overexpression of TINY in Arabidopsis resulted in elevated expressions of both the DRE- and the ERE-containing genes. Moreover, the expression of DRE-regulated genes, such as COR6.6 and ERD10, was up-regulated upon ethylene treatment, and the expression of ERE-regulated genes, such as HLS1, was also increased by cold stress, when the expression of TINY was being induced. These results strongly suggested that TINY might play a role in the cross-talk between abiotic- and biotic-stress-responsive gene expressions by connecting the DRE- and ERE-mediated signaling pathways. The results herein might promote the understanding of the mechanisms of specific DNA recognition and gene expression regulation by DREBs.
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