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Articles by Shamsiah Abdullah
Total Records ( 3 ) for Shamsiah Abdullah
  Nurul Izzati Osman , Asmah Awal , Norrizah Jaafar Sidik and Shamsiah Abdullah
  Lycium barbarum or commonly known as goji or wolfberry is a type of Chinese medicinal herbs which has been consumed since ancient times. In the present study, the best explant and corresponding treatment for callus induction and somatic embryogenesis in this species were determined. Leaves and nodes were used as explants and cultured on different combinations and concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) plant growth regulators. For callus induction, the optimal callus formation effects were found in the treatment of 0.3 mg L-1 2,4-D and 0.1 mg L-1 or 0.3 mg L-1 BAP in Murashige and Skoog’s (MS) media, while 0.1 mg L-1 BAP with either 0.3 mg L-1 or 0.5 mg L-1 2,4-D in MS media were the optimal treatments in nodal explant. As for somatic embryogenesis, after a series of sequential subculture on MS basal media, the treatment of 1.0 mg L-1 2,4-D and 0.1 mg L-1 BAP in MS media during callus induction phase in leaf explant were identified to be the optimum treatment to produce somatic embryos. Through this study, reliable protocols useful for L. barbarum mass propagation through callus culture and somatic embryos formation had been established and verified.
  Norain Mohd Nor , Shamsiah Abdullah , Abdul Rahim Harun , Nor`Aishah Hassan and Sopiah Mat Desa
  Rice is an important agricultural crop particularly in Malaysia. The rice production was affected by many factors including rice blast disease. In attempt to overcome the rice blast disease, genetic analysis study on new crosses of cultivar from Pongsu Seribu 2 and MR264 was used. The objective of this study is to identify segregation pattern of F2 and F3 population resistance to M. oryzae at genotypic and phenotypic level with associated resistance genes linked with SSR markers. SSR markers were used to screen segregating pattern in genotypic level while phenotypic screening was done in greenhouse by observing the disease development to the rice plant. Chi-square results shows a good fit of 1:2:1 for a single gene model which proved the hypothesis of a single dominant gene and 3:1 for the segregation of resistance and susceptibility for M. oryzae but did not segregate with epistasis and two-gene model. SSR markers of RM101, RM206, RM413 and RM495 were significantly associated with blast resistance to M. oryzae in rice. These markers were highly accurate for resistant plant with resistance effect about 12% for phenotypic variation. This study also shows that there was a strong interrelationship between the selected phenotypic and the four markers in this study. Therefore, these studies can be utilized for future references to identify these resistant and susceptible varieties based on polymorphism. This research offered very useful information for local rice breeders in developing blast disease resistant rice cultivar.
  Norrizah Jaafar Sidik , Saiyidah Nafisah Hashim , Yaseer Suhaimi Mohd and Shamsiah Abdullah
  Rockmelon is belongs to one of Cucurbitaceae family. It is widely cultivated for commercialization. In this experiment, three different rockmelon cultivars were selected to be studied. Seed morphology, plant growth yield and fruit quality of rockmelon cultivars (Glamour, Honeymoon and Champion) planted in coco peat were evaluated. Plant was irrigated through fertigation technique and grown under rain shelter. After five weeks, fruit was harvested and tested for ascorbic acid and Total Soluble Solid (TSS) content. Among all three cultivars studied, seed of rockmelon cv. Glamour measured with highest seed biomass (0.034 g). It also gave the best germination response (99%) with highest seedling height (12.14 cm) and widest leaf area (244.5 cm2). For fruit performance, cv. Honeymoon yielded fruit with highest fresh weight (2.24±0.04 kg) and widest diameter (54.86±0.586 cm). However, overall rockmelon cv. Champion remains as the best quality cultivar studied. The fruit measured with thickest mesocarp (37.23±1.60 mm) and contained with highest percentage of ascorbic acid (30.43%) and TSS (16%). As conclusion, all rockmelon cultivars tested have been successfully grown in coco peat and cv. Champion was the best cultivar to be planted as it gave the best fruit value.
 
 
 
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