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Articles by Shaiesta Shah
Total Records ( 4 ) for Shaiesta Shah
  Shaiesta Shah , Sahera Nasreen and N.A. Munshi
  Successful methods to control the damaging weed mold, Trichoderma harzianum (green mold) in mushroom beds are not presently known. The aim of the present study was to determine antifungal potentials of extracts of some selected botanicals against the infection of Green mold (Trichoderma harzianum) associated with the Pleurotus sp. cultivation. Eight botanicals namely Azadiracta indica, Allium sativum, Artemesia indica, Urtica dioeca, Lycopersicon esculentum, Datura stramonium, Mentha spicata and Juglans regia were evaluated in the laboratory for their efficacy against both Pleurotus mycelium and pathogen (Trichoderma harzianum), causing Green mold of mushroom. The efficiency of botanicals against Trichoderma was examined by poison food technique. The percent inhibition produced by botanicals against Trichoderma recorded in vitro, was: Azadiracta indica (34.1%), Allium sativum (28.4%), Artemesia indica (21.8%), Urtica dioeca (22.2%), Lycopersicon esculentum (17.4%), Datura stramonium (17.6%), Mentha spicata (20.8%) and Juglans regia (51.9%). Botanicals showing maximum efficacy against the pathogen and minimum inhibition against the mushroom were further evaluated against Trichoderma infection in mushroom house (in vivo test). In in vivo test, the polybags which receive Azadiracta indica show maximum mean increase in yield (32.8%) over control and exhibited minimum mean disease incidence (27.7%) than control. Ecofriendly management practice, i.e., use of botanicals was studied both in vitro and in vivo which gave better results and these practices can be economical, long lasting and free from residual side effects.
  Shaiesta Shah and Sahera Nasreen
  The aim of the present study was to check the efficacy of various bioagents against green mould (Trichoderma spp.) associated with the Pleurotus spp. cultivation both in vitro and in vivo. Pleurotus specie commonly known as oyster mushroom or dhingri mushroom is one of the most important mushrooms cultivated in india. Four bioagents namely Pseudomonas fluorescence, Streptomyces sp., Bacillus cereus and Bacillus subtilis (3 isolates., Bs-I, Bs-II and Bs-III) were screened against all Trichoderma spp. (T. harzianum, T. viride and T. pseudokoninjii) and Pleurotus in vitro. Antifungal efficiency was examined by streaking method. The percent inhibition produced by bioagents against Trichoderma spp. recorded was: P. fluorescence (35.2 to 41.8%), Streptomyces sp., (12.3 to 30.8%), Bacillus cereus (16.9 to 30.5%), Bs-I (35.1 to 40.9%), Bs-II (36.1 to 44.3%) and Bs-III (42.1 to 51.1%), respectively. Bacterial antagonists showing maximum inhibition against the pathogen and minimum inhibition against mushroom were further evaluated against the Trichoderma sp. in in vivo test in mushroom house. For in vivo test, sterilized lignite inoculated with the bacterial cultures of P. fluorescence, B. subtilis (Bs-I and Bs-II) was thoroughly mixed with the paddy straw substrate at 1, 2 and 3% concentrations, followed by the inoculation of 3 mL spore suspension of Trichoderma at the rate of 1x103 spores mL-1. All tested antagonists produced more yield and reduced the disease intensity than control. The treatments which receive Bs-II as bioagent show maximum increase in yield (25.32 %) and minimum disease incidence (5.7%) at 3% concentration.
  Shaiesta Shah , Sahera Nasreen and P.A. Sheikh
  Various micro fungi associated with Pleurotus spp. were isolated from Mushroom Research and Training Center (M.R.T.C) in SKAUST, Srinagar. Among various fungi collected, the most predominant were those of Trichoderma spp. A total of 49 isolates of Trichoderma spp. were collected during oyster mushroom cultivation. On the basis of cultural and morphological characteristics, Trichoderma isolates were classified into three species, namely Trichoderma harzianum, Trichoderma viride and Trichoderma pseudokoningii. The predominant specie was Trichoderma harzianum (n = 19) followed by Trichoderma viride (n = 17) and Trichoderma pseudokoningii (n = 13). All the three species were distinct from each other in the characteristics such as mycelial growth rate, colony appearance, shape of conidia and conidiophores and branching pattern of phialides. This study has found Trichoderma harzianum to be the main cause of green mold disease of Pleurotus spp.
  Shaiesta Shah , Sahera Nasreen and Shaheen Kousar
  Green mold (Trichoderma spp.) is a devastating disease in the crop production of mushrooms. In India, it has been reported to cause serious crop losses. It is also common contaminant, occurring in mushroom houses in the Kashmir valley. The aim of the present study was to check the in vitro and in vivo efficacy of fungicides against Green mold (Trichoderma harzianum) associated with the cultivation of Pleurotus sajor-caju. Pleurotus sajor-caju is the third most commercially important edible mushroom worldwide. Five fungicides namely Carbendazim, Bitertanol, Hexaconazole, Captan and Mancozeb were evaluated in vitro against Green mold (Trichoderma harzianum) and against the mushroom mycelium as well, by following Poison food technique. The results revealed that the maximum average inhibition of Trichoderma harzianum was recorded in Carbendazim (90.8%), followed by Bitertanol (40.0%), Captan (36.6%) and Hexaconazole (16.1%). The least inhibition (11.7%) of Trichoderma harzianum was exhibited by Mancozeb. It was further observed that Carbendazim exhibited the least inhibition (24.9%) of Pleurotus sajor-caju, followed by Captan (45.5%), Bitertanol (63.0%) and Hexaconazole (74.5%). The maximum inhibition (87.4%) of Pleurotus sajor-caju was exhibited by Mancozeb. Fungicides showing maximum efficacy against the pathogen (Trichoderma harzianum) and minimum efficacy against mushroom (Pleurotus sajor-caju) were further tested against Trichoderma harzianum during an in vivo test in mushroom house. It was observed that all tested fungicides reduced the disease intensity of Trichoderma harzianum and produced more yield than control polybags. Maximum increase in yield (36.9%) over control and minimum mean disease incidence (9.3%) was recorded in treatment which received Carbendazim as the fungicide. Carbendazim was found to be best fungicide, against the infection of Green mold (Trichoderma spp.) disease of mushrooms.
 
 
 
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