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Articles by Shahrear Ahmad
Total Records ( 2 ) for Shahrear Ahmad
  M. N. Amin , Shahrear Ahmad , S. Sultana , M. R. Alam and M. A. K. Azad
  The cultures of proliferating axillary shoots were established on MS medium with different concentrations of either BA alone or in combinations of BA with NAA or IBA and with shoot explants from field grown mature plant of Ixora fulgens. Nodal segments were found to be the best explants for axillary shoot formation on agar gelled MS medium with 0.5 mg l -1 BA+0.1 mg l -1 NAA. On this medium axillary buds showed sprouting within two weeks of incubation. By sequential reculturing and subculturing 15-20 usable shoots (>1 cm length) could be produced from the 2-node shoot segments after 12-15 weeks of culture. Microcuttings taken from In vitro proliferated shoots were rooted on ½ strength MS medium having 0.1-0.5 mg l -1 of either NAA, IBA and/or IAA. It was found that 90% of the plantlets could be established under Ex vitro conditions when they were transferred on specially made plastic tray containing coco-peet as potting mix.
  Muhammad N. Amin , Kamrun Nahar , Firoj Ahmed and Shahrear Ahmad
  The investigation was based on axillary shoot proliferation, culture multiplication and rooting of the in vitro generated and proliferated shoots of Annona. Different strengths of MS and MMS media showed the best result. For axillary shoot proliferation nodal explant from field grown mature plants showed the best results on MMS medium containing 0.2 mg/l BA(6-benzyl adenine) +0.1 mg/l NAA( α-naphthalene acetic acid) . The highest degree of axillary shoot proliferation from nodal explant was found 80% with 6.0±0.42 shoots per culture. Root forming performance of IBA(Indole-3-butyric acid) was found to be the best among three auxins (NAA, IAA/Indole-3-acetic acid, IBA) tested. The highest percentage of root formation was obtained with 2.00±0.21 roots per cutting on the medium containing 0.1 mg/l IBA. At the high concentration (0.1-0.2 mg/l) of all auxins callus formed from the cut margin of the micro cuttings. The regenerated shoots with well developed roots were gradually acclimatized and successfully transferred to the soil under natural conditions.
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