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Articles by Shahla Mansouri
Total Records ( 2 ) for Shahla Mansouri
  Shahla Mansouri , Afshin Amari and Ali Ghanberi Asad
  In order to develop a media to abolish the swarming of proteus without effecting the growth of other bacteria, the anti-swarming activity of 25 plant essential oils or extracts were evaluated on standard and clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, P. vulgaris and P. Penneri, using standard agar dilution method. The most effective anti-swarming agents tested were; the mixture containing equal amounts of essential oil of Ferula gumosa Boiss plus Lavandula officinalis and essential oil of Zataria multiflora Boiss. Essential oil of Zataria multiflora Boiss had antibacterial activity and inhibited the growth of many isolates at concentration of 2%. Whereas the essential oil of Lavandula officinalis plus Ferula gumosa Boiss although had no antibacterial activity at this concentration, completely inhibited the swarming behavior of all the proteus strains tested. This mixture was quite active in the presence of 7.5% blood as in blood agar plates. Essential oil of Zataria multiflora Boiss and Lavandula officinalis plus Ferula gumosa Boiss with good anti-swarming activities and long shelf life, are commercially available and can be used as an effective anti-swarming agent for preparation of media in clinical laboratories for the isolation of more fastidious bacteria in a mixed culture with proteus.
  Shahla Mansouri and Seyd Mohammad Nayebeeaghaei
  Minimum Inhibitory Concentration (MIC) of tellurite was determined for 469 samples of Escherichia coli isolated from clinical samples in human (urinary tract infection, normal fecal flora), or poultry skin using standard agar dilution method. About half of the isolates (47.1%) had MIC of ≤1 μg mL-1 after 24 h of incubation at 35°C. Many of the bacteria that were cultured with low concentrations of potassium tellurite, ≤ 5 μg mL-1, overcome the toxicity of this heavy metal when they were re-incubated for the next 24 h. The MIC for these isolates was increased, so that the difference between the MIC after 24 and 48 h of incubation became significant (p≤0.0026). In medium containing ≥10 μg mL-1 of potassium tellurite, the bacteria formed typical black colonies. No statistically significant difference was found between the MIC of bacteria that where isolated from human, but higher rate of resistance was found for the Poultry skin samples and 25% of these isolates had MIC of ≥160 μg mL-1. Although for nearly half of the isolates potassium tellurite was toxic at low concentration (≤1 μg mL-1), higher MIC was observed for the rest of isolates (10-400 μg mL-1) and is an indication of the widespread resistance to potassium tellurite in E. coli in this region.
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