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Articles by Shahida Hasnain
Total Records ( 15 ) for Shahida Hasnain
  Rida Batool and Shahida Hasnain
  Twelve bacterial strains of family Enterobacteriaceae were isolated from different biofilms. Total nine biofilms were collected from polluted as well as from non-polluted environments. Isolates were purified and characterized both morphologically and biochemically. Total eleven strains were affiliated with Enterobacter while only one showed affinities with Serratia. Isolated strains of Enterobacter and Serratia were used to inoculate the seedlings of Zea mays in mono and mixed culturing. The growth parameters of Zea mays seedling and soil aggregation were recorded. Monoculture inoculations caused an increase in all length parameters i.e., shoot length, root length, seedling length of Zea mays seedlings. Number of roots decreased and number of leaves remained constant. When all these strains were used as mixed culture, a decrease in all length parameters i.e., shoot length, root length, seedling length was observed while number of roots increased and number of leaves decreased. Monoculture inoculation resulted in increase in weight of aggregates on roots and causing decrease in weight of aggregates in soil. In mixed culture inoculation, weight of aggregates on roots as well as in soil was decreased over control.
  Amina Munir , Iram Munir , Shazia Afrasyab and Shahida Hasnain
  Five bacterial strains of Azospirillum (As-2, As-4, As-5, As-6 and As-8) were used both as monocultures and mixed cultures (twenty-six combinations for each plant) for the inoculation of Triticum aestivum (non leguminous) and Vigna radiata (leguminous) seeds. Inoculated as well as non inoculated seeds were germinated and grown under controlled temperature and light conditions for 10 days. Growth parameters revealed that in both plants, mono culture inoculations manifested the better growth as compared to non inoculated treatments. Mixed cultures inoculation 3j (three strain combination) and 4a (four strain combination) in T. aestivum and 4a (four strain combination) and 3i (three strain combination) in V. radiata exhibited synergistic responses as compared to control treatment. Stimulatory effects were more pronounced in non leguminous seedlings relative to leguminous one.
  Kamran Shaukat , Shazia Afrasayab and Shahida Hasnain
  Bacterial strains of Pseudomonas (Ps1, Ps2, Ps3, Ps4, Ps5) Azotobacter (Ab1, Ab2, Ab4, Ab5, Ab6) and Azospirillum (As2, As4, As5, As6, As8) were used to inoculate the seeds of Helianthus annus var SF-187. Inoculated and non-inoculated seeds were germinated and grown under field conditions until at maturity. Two harvests were taken one after 7 days of germination and other at maturity i.e., after 4 months of sowing. All the bacterial inoculations provoked germination in Helianthus annus. Maximum increase was manifested by Ps5 (223.63%). In general strains of Pseudomonas promoted plant growth more then Azotobacter and Azospirillum, plant height was enhanced by Ab6 (68.66%) and number of leaves was increased by Ps1 (63.63%) after 7 days of germination. However Ps2 strain exhibited maximum enhancement in plant height (57.33%), while Ab1 manifested maximum enhancement in number of leaves (49.01%) after 4 months of sowing. In case of yield parameters As8 manifested maximum increase in number of flowers (128.57%), Ps5 increased diameter of flowers (134.05%), Ab1 weight of seeds (57.00%) and Ab4 (30.35%) manifested enhancement in oil content when compared with control. Biochemical parameters were also increased with bacterial inoculations soluble protein content was enhanced by Ps5 (321.55%), after 7 days of germination and Ab2 (345.45%) after four months of sowing, auxin contents content was enhanced by, Ps1 (1138%) and Ab5 (845.50%), peroxidase activity was enhanced by Ps5 (321.55%) and Ab2 (345.45%) and acid phosphatase activity was increased by Ps1 (97.13%) and As8 (313.33%), after 7 days of germination and after four months of sowing when compared with that of control. These results demonstrate that these strains can be used for enhancing biomass as well as yield parameters.
  Muhammad Faisal and Shahida Hasnain
  Not available
  Muhammad Faisal and Shahida Hasnain
  The colonization potential of plant growth promoting bacterial strains (Ochrobactrum intermedium, Bacillus cereus and Brevibacterium sp.) on Triticum aestivum and Helianthus annuus roots. The roots of Triticum aestivum were heavy colonized with Ochrobactrum intermedium and Bacillus cereus as compared to Brevibacterium. Ochrobactrum intermedium mainly colonized the rhizoplane while Bacillus cereus was present both on the rhizoplane and near the root zone. Ochrobactrum intermedium and Bacillus cereus were found to be present both on the rhizoplane as well as near root zones of both crops used (Triticum aestivum and Helianthus annuus) while Brevibacterium was found in the form of groups in the rhizosphere of Helianthus annuus roots. From the results of both crops it was observed that the number of Ochrobactrum intermedium cells were more where some root exudates seen.
  Tanveer Zahra Mirza , Asama Mahmood , Anjum Nasim Sabri and Shahida Hasnain
  The present study deal with the germination analysis of spores of different div mutants (divI, divII, divIV and divV) of Bacillus subtilis in ALA and AGFK systems and the relationship of these mutations with ger genes. Eight B. subtilis strains PY79 (wild type) and 07 different div mutants were the subject of this study. All div mutant strains produce germination defective spores. Their germination response was analyzed in both ALA and AGFK systems as well as in the presence of glucose, fructose and combination of both in ALA system. Inhibition effects of HgCl2 and NaN3 and reversal by mercaptoethanol and aqueous washing, respectively, were studied. On the basis of these results 1A292 (div IVB), 1A314-(div V) and 1A315(div V) were aligned with gerD, gerA and gerC mutations, respectively. Peptidoglycan components (hexoamines, techoic acid, diaminopimelic acid) of germination defective spores exhibit variation from wild type, hence changes in wall composition leads to defects in cell division and then spore germination. These studies support our earlier finding (the bases of this project) that div mutants (previously div IVA which was aligned to gerB , now div IVB and div V) exhibit pleiotrophic effects.
  Sikander Sultan and Shahida Hasnain
  In order to study the genetic basis and mechanism of chromate resistance in chromate resistant bacteria, conjugal transfer as well as curing of plasmids in these bacteria and its effect on chromium uptake are being investigated. From the effluent of Shafiq Tannery, Kasur, Pakistan, four bacterial strains STCr-1, STCr-2, STCr-3 and STCr-4 which could endure 40 mg mL-1 of potassium chromate in nutrient agar medium were isolated. All of them were gram negative, aerobic and motile rods. One strain STCr-1 was identified as Ochrobactrum species by 16S rRNA gene sequence homology. Each strain harboured a single conjugative plasmid, which conferred resistance to chromate. Maximum plasmid transfer was recorded after 24 h of mating except for plasmid residing in STCr-3 (pSH1322), which transferred maximally after 16 h of mating. The plasmids harboring STCr-1 (pSH1320), STCr-3 (pSH1322) and STCr-4 (pSH1323) manifested highest transfer frequency at donor:recipient ratio 1, while plasmid resident of STCr-2 (pSH1321) preferred donor:recipient ratio 5. Transfer frequency of plasmids pSH1320 and pSH1322 was maximal at 28°C and that of pSH1321 and pSH1323 at 37°C. Optimum pH for plasmid transfer was 8 for these plasmids except pSH1320, which opted for pH 6 or 7. Curing of chromate resistant plasmids from these strains was achieved with SDS at high temperature and curing of plasmid was associated with the loss of chromate resistance phenotype. A comparison of Cr uptake by the parental strains and their cured derivatives revealed that plasmids in these strains express high level resistance to chromate by exerting stringent control on the accumulation/uptake of Cr.
  Iram Ghaffar , Basharat Ali and Shahida Hasnain
  The main aim of this study was to observe the effect of different hormonal combinations on regeneration of callus of Gomphrena globosa L. For this purpose callus was obtained from seeds G. globosa inoculated on MS medium supplemented with 4 mg L-1 2, 4-D and 10% coconut milk. After callus formation callus was inoculated on Murashige and Skoog’s medium supplemented with different combinations of BAP, NAA and GA3 to observe different responses such as regeneration, callus friability, callus proliferation and pigmentation. In BAP and NAA root regeneration was observed at 1.5 mg L-1 BAP+1 mg L-1 NAA whereas rest of the combinations showed callus proliferation. In BAP and GA3, root regeneration was observed in most of the combinations and some combinations also showed shoot induction. Shoot regeneration was observed on 0.5 mg L-1 BAP + 7 mg L-1 GA3 and 1 mg L-1 BAP + 0.2 mg L-1 GA3. The effect of all these combinations on auxin, acid phosphatase and soluble protein content was also observed.
  Shazia Afrasayab and Shahida Hasnain
  Salt tolerant bacteria were used to assess their potential for stimulating the growth of Triticum aestivum var. Inqlab 91. Certified seeds of Triticum aestivum var. Inqlab 91 were inoculated with monocultures (ST-1, ST-2, ST-3, ST-4, rhizosphere bacteria; HT-1, HT-2, HT-3, histoplane bacteria) and different combinations of mixed cultures of bacteria. Inoculated and non-inoculated seeds were germinated and grown under NaCl stress (0, 100 mM NaCl) for 10 days. After that growth measurements (length and weight parameters) were taken and Na+/K+ contents, soluble protein content, auxin content and enzyme activities (peroxidase and acid phosphatase) were determined. Generally, with the application of NaCl, reduction in germination and growth parameters, whereas increase in Na+/K+ contents, auxins content, soluble protein content as well as enzymes activities were observed. Bacterial inoculations stimulated germination and growth, over non-inoculated respective treatments, especially under saline conditions. Mixed culture inoculation with rhizosphere bacteria (some combinations) and histoplane bacteria was more effective in stimulating seedling growth than monoculture inoculations at 100 mM NaCl. Bacterial inoculations also enhanced fresh weight per seedling, auxin, soluble protein and K+ contents of seedlings. Whereas decreases in dry weight accumulation, Na+ content and activity of enzymes, at 100 mM NaCl with bacterial inoculations were recorded. From these results improved growth of seedlings could be related with increased auxin content but decreased dry weight increment, Na+ content and enzyme activities.
  Muhammad Faisal and Shahida Hasnain
  Two chromium resistant bacterial strains i.e. Bacillus sp. S6 and Staphylococcus sp. CrM-3 were used in conjunction with Eichornia crassipes to study the effect of chromate removal from chromium contaminated waste waters. Bacterial strains resulted in reduced uptake of chromate into inoculated plants, which was 17.24 and 11.29% with S6 and CrM-3 respectively, at an initial Cr (VI) concentration of 100 μg ml‾1 as compared to non-inoculated control plants. Different heavy metals (CuSO4, NiSO4, MnCl2 and Pb (NO3) 2) at a concentration of 100 μg ml‾1 were added in chromate-supplemented solution to check their effects on chromate uptake by the plants. At different chromate concentrations the uptake of Cr (VI) into the plants was 28.73, 5.38, 7.15, 12.29 and 15.35% less at an initial Cr (VI) concentration of 100, 300, 500, 1000 and 2000 μg ml‾1 in comparison to metal free condition. Cr (VI) uptake by the Eichornia crassipes was occurred at different pH tested, but maximum uptake was observed at pH 5. Nevertheless the bacterial strains caused some decrease in chromate uptake into the plants but the combined effect of plants and bacterial strains conduce more removal of Cr (VI) from the solution.
  Muhammad Faisal and Shahida Hasnain
  Three chromium resistant bacterial strains originally isolated from chromium-contaminated wastewater, were tested in the field experiments for the growth promotion of Helianthus annuus in the presence of different chromium salts. Much reduction in seed germination was observed under Cr(VI) stress but all bacterial strains promoted seed germination with respect to non-inoculated control. Inoculation increased plant length in sunflower by 14 to 26%. Sunflower plants showed medium and high increases in their fresh weight in response to inoculation with these strains. Inoculation with these strains increased dry weight up to 15% as compared to non-inoculated control. The contribution of the strains to promote auxin content in these plants varied from 88% when sunflower plants were inoculated with strain CrT-6, to 174% when strain CrM-6 was used. All the three strains showed similar effects in respect to chromium accumulation when supplied with 300 μg g-1 of K2CrO4.
  Muhammad Faisal and Shahida Hasnain
  A chromium resistant bacterial strain S-6 was isolated from chromium-contaminated soil. On the basis of different morphological, biochemical characteristics and 16S rRNA gene analysis the strain was identified as Bacillus cereus S-6. Hexavalent chromium resistance of the strain showed that it could tolerate very high concentration of K2CrO4 both in nutrient medium (25 mg mL-1 in nutrient broth and 50 mg mL-1 on nutrient agar) as well as in acetate-minimal medium (15 mg mL-1). Chromate uptake and reduction studies revealed that the strain Bacillus cereus S-6 not only accumulate but can also reduce large amount of toxic Cr (VI) in to Cr (III). Heavy metals (Ni, Mn, Zn, Cu and Co) at low concentration did not affect the reduction capability of the strain. Strain could also reduce hexavalent chromium in industrial effluents.
  Kamran Shaukat , Shazia Affrasayab and Shahida Hasnain
  Bacterial strains of Pseudomonas (Ps1, Ps2, Ps3, Ps4, Ps5) Azotobacter (Ab1, Ab2, Ab4, Ab5, Ab6) and Azospirillum (As2, As4, As5, As6, As8) were used to inoculate the seeds of Triticum aestivum Var Inqalab-91. Inoculated and non-inoculated seeds were germinated and grown under field conditions until at maturity. All the bacterial inoculations provoked germination and growth parameters in Triticum aestivum, however Azotobacter strains exhibited more pronounced effect after 7 days of germination such as plant height (55.19%), number of leaves (50.01%) and Pseudomonas strains after 4 months of sowing such as plant height (82.60%), spike length (212.73%) and number of spikes (26.66%), when compared with that of control. All strains increased soluble protein content both after 7 days of germination and at maturity; As6 (64.95%) exhibited maximum increase after 7 days and Ab2 (791.79%) after four months of sowing. Auxin contents were also increased with the inoculation of bacterial strains when compared with that of control however, Ps3 (162.96%) exhibited maximum increase after 7 days and As2 (263.33%) after four months of sowing (at maturity). All strains increased peroxidase activity in Triticum aestivum As6 (109.55%) manifested maximum increase after 7 days and Ps1 (1436.09%) after four months of sowing. Acid phosphates activity was also enhanced with all the bacterial strains however As2 (263.54%) showed maximum increase after 7 days, whereas after four months of sowing Ps4 (146.95%) manifested maximum increase in this parameter. These results demonstrate that these strains can be used as biofertilzer for enhancing biomass as well as yield parameters.
  Saira Anwar , Anjum Nasim Sabri and Shahida Hasnain
  The present study deals with the effect of temperature (37 and 45°C) in combination with varying pH (6, 7, 8, 9) after 24 and 96 h of incubation on cell morphology, staining behavior and growth rate of PY79 (wild type) and sporulation defective mutant strains (5, 19, 96, 99) of bacillus. Strains were isolated from local polluted area around Lahore. All the strains were gram positive spore former aerobic rods. In majority of strains the optimum temperature was 37°C and pH 7. Frequencies of different cell types differ at different temperatures and at varying pH. Cell degeneration and lysis was more in sporulation defective strains (5, 19, 96, 99). Spores which were formed by the bacterial isolates 5, 19, 96 and 99 were different from wild type and were unable to germinate.
  Uzma Iqbal , Saqib Mehmood , Muhammad Faisal and Shahida Hasnain
  Short stature may be the normal expression of genetic potential, in which case the growth rate is normal, or it may be the result of a condition causing growth failure with a lower-than-normal growth rate. Short stature and puberty failure are primary characteristic of Turner Syndrome. The study was aimed to find out the chromosomal compliment of girls with suspected turner, having short stature and failure of puberty as phenotypic manifestations. Karyotype analysis was performed in 25 patients by standard procedures, using metaphase chromosome preparations from phytohemagglutinin stimulated blood lymphocytes. In each individual, conventional G-banding was also performed to obtain high resolution of banding pattern. 50% of the girls showed X-chromosome monosomy (45XO karyotype), 35% showed mosaicism 45XO/46XX and 10% showed structural abnormalities of X-chromosome, while 5% of the girls showed normal karyotype.
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