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Articles by Sh. A. Gabr
Total Records ( 4 ) for Sh. A. Gabr
  W.A. Khalil , Sh. A. Gabr , Sh. M. Shamiah , A.M.A. El-Haif and A.E. Abdel-Khalek
  This study aimed to compare the efficiency of three cryodevices (Conventional Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They were vitrified and thawed according to each cryodevice used. Then they were in vitro matured, fertilized and cultured to blastocyst stage. Survival and quality, in vitro maturation, fertilization rate and blastocyst production rates of vitrified immature buffalo oocytes were assessed. Results showed that total survival rate of the vitrified oocytes was higher (p<0.05) for SP and HS (96 and 95%) than CS (80%). Recovery rate of normal oocytes relative to vitrified or survival oocytes was the highest (p<0.05) for SP compared to other cryodevices. In vitro maturation rate (oocytes at metaphase II stage) was lower (p<0.05) for all vitrified oocytes than fresh (61.1 vs. 27.8-44%). SP showed higher (p<0.05) maturation rate (44%) than HS and CS (34.4 and 27.8%, respectively). Cleavage rate was lower (p<0.05) for all vitrified oocytes than fresh oocytes (37.7-58.9% vs. 70.8), being higher (p<0.05) for SP and HS (55.8 and 58.9%, respectively) than for CS (37.7%). Blastocyst production rate relative to cleaved oocytes was lower (p<0.05) for vitrified than fresh oocytes (5.9-14.3% vs. 31.3) but the differences among vitrification cryodevice were not significant. In conclusion, immature buffalo oocytes can vitrified successfully by spatula, conventional straws and Hemi-straws. Spatula device is a reliable alternative, inexpensive and easy to assemble for vitrification of immature buffalo oocytes. Also, vitrification spatula has the largest effective holding capacity.
  Sh. A. Gabr , Sh. M. Samiah and W.M. Nagy
  The present study aimed to evaluate the effect of leptin addition (0, 10, 20 and 30 ng mL-1) to maturation media on in vitro maturation (IVM) and fertilization (IVF) of dromedary camel oocytes. Ovaries were collected from slaughtered animals, oocytes were collected by slicing technique and matured in TCM-199 medium with different leptin levels in CO2 incubator (5% CO2) at 38.5°C and high humidity for 42 h. After maturation, oocytes were categorized as Germinal Vesicle (GV), Germinal Vesicle Breakdown (GVBD), metaphase-I (MI), metaphase-II (MII) and degenerated oocytes. Epididymal spermatozoa were recovered from camel testes immediately after slaughter. In vitro fertilization was carried out for oocytes matured in TCM-199 with 10% FDCS plus 20 μg mL-1 of leptin. Spermatozoa and oocytes were co-incubated at 38.5°C in a moisture atmosphere of 5% CO2 in air for 20-24 h. The cleaved oocytes were examined after five days of culture for cleavage stages (2, 4 and 8-16 cell, morula and blastocyst stages). Results revealed that supplementation of leptin (20 mg mL-1) showed (p<0.05) the highest percentage of oocytes at MII (58.8%) and the lowest percentages of those at GV (7.8%), GVBD (9.8%), MI (10.8%) and degenerated oocytes (12.7%) as compared to control medium (40.0, 10.9, 11.8, 14.5 and 22.7%, respectively) and other levels of leptin. Supplementation of leptin, matured with 20 ng mL-1 leptin increased fertilization rate (27 vs. 25.3%) and blastocyst rate (3.6 vs. 1.4%) as compared to the control medium. In conclusion, supplementation of leptin to maturation medium (TCM-199) at a level 20 ng mL-1 increased in vitro nuclear maturation rate of immature oocytes and their fertilization rate and blastocyst production.
  Sh. A. Gabr , A.E. Abdel-Khalek and I.T. El-Ratel
  This study aimed to evaluate the influence of collection techniques (dissection, aspiration, slicing and aspiration plus slicing) on yield and quality of buffalo oocytes in vitro matured (IVM) in TCM-199 or Ham’s F12 with or without hormones (follicle-stimulating hormone, FSH and estradiol, E2). Recovery Rate (RR) and category of the collected oocytes were determined and only Cumulus Oocyte Complexes (COCs) were matured in vitro. Results showed that RR was 84.6, 83.3, 72.7 and 52.0% for aspiration plus slicing, slicing, aspiration and dissection technique, respectively (p<0.05). Percentage of COCs was higher by slicing than aspiration, aspiration plus slicing and dissection (63.2 vs. 51.3, 51.2 and 42.0%, p<0.05). The corresponding percentages of expanded oocytes were 29.9, 30.3, 27.6 and 32.7%, respectively (p<0.05). Percentage of oocytes arrested at metaphase-II (MII) was higher (67.1%, p<0.001) while those at Germinal Vesicle (GV) (8.1%, p<0.001) and Deg. (9.1%, p<0.01) were lower in TCM-199 than in Ham’s F12. Hormonal addition increased percentage of oocytes arrested at metaphase-I (MI) and MII (p<0.05 and p<0.001, respectively) and decreased those at GV and Deg (p<0.001). Such results may indicate efficacy of slicing technique as a collection method on quantity and quality of buffalo oocytes. Also, in vitro maturation of buffalo oocytes was improved in TCM-199 supplemented with hormones (FSH and E2).
  A.E. Abdel-Khalek , H.K. Zaghlool and Sh. A. Gabr
  This study aimed to evaluate the effect of daily oral administration of free L-carnitine (LC) for 3 months on digestibility coefficients, blood parameters and semen traits of Friesian bulls. Total of 9 bulls (360±32.1 kg LBW and 20±1.4 months of age) were assigned into 3 groups. Bulls in G1, G2 and G3 were fed the same diet and kept under the same conditions but differed in LC level (0, 1 and 2 g h-1 day-1, respectively). Bulls were received LC for 3 months (treatment period). At the end of treatment, semen was collected from bulls twice a week for 12 weeks and evaluated for ejaculate volume, sperm progressive motility, livability and abnormality, sperm concentration and total sperm output. Also, digestibility coefficients were performed and blood samples were collected. Concentration of Total Proteins (TP), albumin (AL), globulin (GL), urea-N, cholesterol (CHO), glucose and Total Lipids (TL), activity of AST and ALT and testosterone were determined in blood serum. Results show that bulls in G3 showed highest (p<0.05) digestibility coefficients. Only concentration of TP and GL increased (p<0.05), AL/GL ratio and CHO and TL concentrations reduced (p<0.05) in LC groups. All semen characteristics improved (p<0.05) in LC groups, being better (p<0.05) in G3 than in G2. Serum testosterone concentration was higher (p<0.05) in G2 and G3 than in G1. In conclusion, oral dose of LC at a level of 2 g h-1 day-1 for 3 months had impact to achieve high quality semen to spread the use of artificial insemination with bulls of high fertility.
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