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Articles by Seyit Aydin
Total Records ( 4 ) for Seyit Aydin
  Seyit Aydin , Abdulkadir Ciltas , Hasan Yetim and Ihsan Akyurt
  This study investigates chronic and sporadic Micrococcus luteus infections of 3 and 4 -year-old broodstock and 2-year- old juvenile cultured rainbow trout (Oncorhynchus mykiss Walbaum) during February through April 1993-1996. Infection had not been observed from 1997 to 2003. Bacterial isolates were identified each year and were tested to determine sensitivity to chemotherapeutants. Infections caused lesions on the skin and caudal fin, and internal organs such as muscle, liver and spleen. In 1995, naturally infected 2-year-old fish were examined for haematological variables and compared to healthy fish. Leucocytosis and thrombocytosis occurred as well as a reduction in haemoglobin and haematocrit values of infected fish. In 1996, histopathological examination demonstrated pathological changes in heart, liver, spleen, gill, muscle, kidney and intestinal tissues of juvenile rainbow trout. The minimum bactericidal concentrations (MBCs) of formalin (90 %) + ethyl alcohol (10 %) mixture and enrofloxacin against pathogenic bacterium isolated in 1996 were 3 g/ml and 0.025 g/ml, respectively. Formalin + ethyl alcohol mixture baths and oral applications of enrofloxacin were successfully used for the treatment of naturally infected fish in 1996. Pathogenicity of the M. luteus isolated from crucian carp (Carassius carassius L.) was tested by intramuscular injection using healthy rainbow trout.
  Nejdet Gultepe and Seyit Aydin
  Pseudomonas elongata was isolated from naturally infected scattered mirror carp (Cyprinus carpio) and its pathogenicity was tested by intramuscular injection. The infection caused mortality in scattered mirror carp with gross clinical abnormalities such as dark coloured of a location on body surface, cataract in eyes, haemorrhagic damage of liver, irrigation in kidney, anemia, swollen intestine, fins rot and hyperaemia in operculum and skin. Lethal Dose50 (LD50) of Pseudomonas elongata was calculated 2.24x105. No significant difference was obtained among enumered of pathogenic bacteria isolated from gill, liver, kidney tissues and total pathogenic bacteria. Sensitivities of Pseudomonas elongata against 50 chemotherapeutants were tested. Minimum inhibitory concentrations of enrofloxacin and chloramine T to the isolate were calculated 5 mL L-1 and 10 mg L-1, respectively. Best treatment method was no mortality performed with orally enrofloxacin application and chloramine T bath.
  Musa Bulut and Seyit Aydin
  Arcobacter cryaerophilus was isolates from nautrally infected rainbow trout, (Oncorhynchus mykiss Walbaum) and its pathogenicity was tested by intramuscular injection into healthy 1-year-old normally pigmented rainbow trout and albino crosses. Experimental infections caused deaths with gross clinical abnormalities such as exophtahlmia, liver damage, bloody hemorrhagic kidney and heart and swollen intestines. No significant differences in deaths were observed between the two infected fish groups. Significant increases were observed in the serum glutamate pyruvate transaminase (GPT) level of both the experimentally infected albino crosses and rainbow trout groups. The mean serum glutamate oxalacetate transminase (GOT) value in blood of infected rainbow trout was significantly higher than in healthy fish, but GOT activity in blood of experimentally infected albino crosses was significantly less than that of healthy fish. Glucose (GLC) levels in blood of both experimentally infected albino crosses and rainbow trout were significantly less than in healthy fish. Cholesterol (CHOL) concentrations in serum were not significantly different among the four treatments. Serum triglyceride (TG) concentration in blood of experimentally infected normally pigmented rainbow trout group was significantly higher than in healthy fish.
  Seyit Aydin and 1Abd?lkadir ?ilta
  In the present study Aeromonas hydrophila outbreaks occurred for 3 months (July, September and October) in 1996 about 15-20 days after flooding in juvenile cultured rainbow trout (Oncorhynchus mykiss Walbaum) were investigated. Sensitivities of four isolates of A. hydrophila against 16 chemotherapeutants were determined. Minimum bactericidal concentrations (MBCs) of formalin to the isolates were between 6.4-8 g/ml. 23 g/ml dose of potassium per manganate (KmnO4) and 15 g/ml dose of Chloramine-T were effective to inhibit one of the isolates but not to the other isolates. Oral applications of ofloxacin or flumequine following bath disinfections with formalin controlled the natural infections. Pathogenicity of three isolates of A. hydrophila was tested by intramuscular injection into healthy 1-year-old rainbow trout. The lethal dosages of 50 % end point (LD50) for the isolates were calculated as 4 x104, 2 x105 and 5 x105, respectively. Natural and experimental infections caused dross clinical abnormalities such as abnormal movements, anorexia, darkening skin, pale and swollen gills, cherry-red coloured spleen, necrosis in liver, haemorrhage in kidney and serous exudates in intestine. Histopathological examination demonstrated pathological changes in gill, brain, heart, kidney, liver and intestinal tissues of naturally infected juvenile rainbow trout. Glutamate oxalacetate transaminase (GOT) enzyme and bilirubin (BIL) levels in blood serum of naturally infected rainbow trout were significantly higher than in healthy fish. No significant increases were observed in the serum L-lactate dehydrogenase (LDH), blood urea nitrogen (BUN) and creatinine (CRE) levels of diseased fish group. There was no significant a decrease in the mean concentration of glucose (GLC) of the naturally infected fish group.
 
 
 
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