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Articles by Sergun Dayan
Total Records ( 2 ) for Sergun Dayan
  Suleyman Cenkci , Mustafa Kargioglu , Sergun Dayan and Muhsin Konuk
  The objective of this study were to investigate present endangered status and propagation of Thermopsis turcica, which is a very unusual plant species for Turkey. T. turcica grows in a very narrow area located between the Eber and Aksehir Lakes. The field studies indicated that holding unhealthy seeds due to pest infestation and clearing new agricultural fields are two major threatened factors for T. turcica. In sterile conditions, concentrated sulfuric, hydrochloric and nitric acid pre-treatments for 0, 30, 60, 90 and 120 min used to germinate T. turcica seeds which were only obtained from small Eber populations. 99% seed germination within a few days was achieved from 120 min. sulfuric acid pretreatment; whereas the hydrochloric and nitric acid treatments did not have effect on the germination (<20%) of T. turcica seeds. In non-sterile conditions, however, a maximum 61% germination was obtained from 120 min sulfuric acid pretreated seeds. Although 20% non-sterile seedlings were survived, 86% in vitro seedlings acclimatized to green house conditions were alive after 14 weeks. This propagation technique, in vitro germination-acclimatizing to the soil, could be used to propagate endangered plant species suffering from germination and seedling development.
  Ciler Meric , Necmettin Guler , Hayati Arda and Sergun Dayan
  Nuclear DNA content of Trachelium jacquinii subsp. dalgiciorum, an endemic taxon, was analysed using flow cytometry. Plants were collected from a natural population. While some plants were prepared as herbaria material, others were placed in pots and incubated in growth chamber. Fresh leaves for flow cytometry analysis were chopped in MgSO4 buffer (with dithiothreitol, propidium iodide and triton x-100) on ice in a Petri dish and stained with propidium iodide (with RNase-DNase free). Stained nuclei were analysed on an EPICS XL (Beckmann Coulter) model flow cytometer. The mean DNA content per plant was based on 10000 scanned nuclei. Nuclear DNA content (2C-value) of Trachelium jacquinii subsp. dalgiciorum was found as 3.44±0.14 pg (1C-value 1.72 pg). This study contributes present data on nuclear DNA content of angiosperm taxa.
 
 
 
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