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Articles by Sebnem Pamuk
Total Records ( 4 ) for Sebnem Pamuk
  Belgin Siriken , Ozgur Cadirci , Gokhan Inat and Sebnem Pamuk
  The aim of the present study was to evaluate, the incidence of some pathogen and hygienic microorganism in meatball, cream cake and Turkish delight samples. A total 63 sample (25 raw meatball, 17 cream cake and 21 Turkish delight samples) were analyzed. For this aim, drop and spread method were applied on to specific media. As a result, Salmonella sp. were present in meatball and B. cereus was found in cream cake samples. Coagulase positive staphylococci were present meatball samples up to 104 cfu g-1 levels. Sulphite reducing bacteria were not detected in any samples analyzed. Meatball samples may be potential risk for staphylococcal intoxication to consumer during shelf-life. Cream cake samples could be potential risk for B. cereus. Additionally, analyzed samples were also contaminated with other enteric and spoilage bacteria. Majority of Turkish delight samples were of satisfactory/acceptable microbiological quality. Contrast to Turkish delight, microbiological qualities of the meatball and cream cake samples analyzed were unsatisfactory and the product could be cause of food poisoning. To minimize contamination, GHP or GMP and HACCP systems could be applied to the control of the pathogenic and spoilage bacteria at all stages of manufacture, storage, transport and retail step.
  Sebnem Pamuk and Sadi Akgun
  The aim of this present study is to detect thermophilic Campylobacter species in chicken carcasses sold unpackaged in Afyonkarahisar and to confirm C. jejuni isolates by using a PCR test based on the ceuE gene. A total of 210 chicken carcasses were collected from various markets from May till the end of October in 2004. Thermophilic Campylobacter sp. was isolated from 141 (67%) of 210 chicken carcass samples by classical culture method. The identification of 419 thermophilic Campylobacter sp. isolates obtained from chicken carcass samples were; 57.04% (239) C. jejuni, 40.57% (170) C. coli and 2.39% (10) C. lari. PCR method was applied to 101 of 239 C. jejuni isolates obtained from the chicken carcasses (n = 101). According to it 62 (61.38%) of 101 isolates were confirmed by PCR. About 28 (45.16%) of 62 isolates were confirmed to be C. jejuni were detected to be resistant to nalidixic acid.
  Sebnem Pamuk , Zeki Gurler , Yeliz Yildirim and Belgin Siriken
  In this study, a 100 carp fish that offered for sale in public bazaar in Afyonkarahisar were microbiologically examined in 2009 period (15 June to 15 July). In most of these samples, aerob total count was found between 107 and 108 kob mL-1. Coliform count ranked 2nd after the aerob total count. Most of micrococcus and staphylococcus were found at 104-105 level. Although, the count of enterococcus was generally between 105 and 106, the count of enterobacter count was generally 106-107. Mold was not isolated from samples. Coliform bacteria level was 107-108 kob mL-1. However, E. coli was under the detection level in most of the samples while it was 104-105 in the rest. On the other hand, Salmonella highly important for food infections was determined in 3% of the carp fish samples.
  Zeki Gurler , Sebnem Pamuk and Ulas Acaroz
  In the present study, several microbiological and physicochemical properties of the potable/utility water in central Afyonkarahisar and its counties were investigated. A total of 162 potable/utility water samples collected in June to August 2011 were used as the study material. Drop plate method was used for Total Aerobic Mesophilic Bacterial (TAMB) counts and psychrotrophic microorganism counts whereas membrane filtration method was used in the identification of Pseudomonas sp., enterobacteria, enterococcus, coliform bacteria, E. coli and Cl. perfringens. The microbiological findings of the study indicated that the total aerobic mesophilic bacterial counts in wells were <1 cfu/100 mL in 32% of all samples and <1cfu/250 mL in utility and spring-tap water samples. The aerobic bacterial count was 1-10 cfu/250 mL in 35% of the utility water samples, 11-100 cfu/250 mL in 24% and 101-300 cfu/250 mL in 9% whereas it was 1-10 cfu/250 mL in 56% of spring/tap water samples and 11-100 cfu/250 mL in 7% in addition to the fact that no samples were identified to have bacterial counts in the range of 101-300 cfu/250 mL. The well water bacterial count was determined as 1-10 cfu/250 mL in 46% of the samples, 11-100 cfu/250 mL in 15% of the samples and 101-300 cfu/250 mL in 11% of the samples. The psychrotrophic organism count was determined as <1 cfu/100 mL in well water samples and <1 cfu/250 mL in spring/tap water samples in 40% of the cases. The aerobic microorganism count was determined as 1-10 cfu/250 mL in 41% of the utility water samples as 11-100 cfu/250 mL in 13% and 101-300 cfu/250 mL in 7% whereas the aerobic microorganism count was determined as 1-10 cfu/250 mL in 50% of the spring/tap water samples and as 11-100 cfu/250 mL in 6% in addition to <1 cfu/250 mL in 101-300 cfu/250 mL range. The well water samples were determined to have 1-10 cfu/100 mL of aerobic microorganisms in 41% of the samples, 11-100 cfu/100 mL in 17% of the samples and 101-300 cfu/100 mL in 7% of the samples. The water samples (utility/spring-well water) collected from Afyonkarahisar and its provinces were determined to be contaminated by coliform bacteria (17%, 28 samples), enterobacteria (15%, 24 samples), enterococcus bacteria (12%, 20 samples), E. coli (7%, 12 samples), Pseudomonas sp. (13%, 21 samples) and sulfite reducing microorganisms-Cl. perfringens (14%, 23 samples) in the study. All the samples under investigation were determined to be clear, odorless with specific taste and color although some samples did not comply with the legislation in terms of the microbiological criteria. The results of the physicochemical analysis indicated that the average pH, turbidity (NTU), conductivity (μohm/cm) and total hardness (French Hardness Degree, f0) values of the 162 samples were 7.28; 0.497; 362.11; 22.78, respectively. The average nitrite, nitrate, arsenic, chlorine, ammonium, floride, calcium and sulfate concentrations of the samples were determined as 0.09 mg L-1, 17.25 mg L-1, 0.26 μg L-1, 0.83 μg L-1, 0.15 mg L-1, 0.94 mg L-1, 18.11 mg L-1 and 20.42 mg L-1 respectively.
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