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Articles by Sebahattin OZCAN
Total Records ( 2 ) for Sebahattin OZCAN
  Iskender PARMAKSIZ and Sebahattin OZCAN
  The species of the section Oxytona Bernh., classified under the genus Papaver L., are of great importance due to their medical alkaloid contents such as morphine, codeine, and thebaine. A clearly established classification system is not available for Oxytona species. The aim of this study was to find relationships among 53 accessions belonging to 3 important Oxytona species, i.e. Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., using 19 different morphological characters, 1 chemical character, and 15 random amplified polymorphic DNA (RAPD) markers. Nineteen characters, including bract and sepal leaves, petal marks, and capsule shape and size were analysed, and distinct morphological differences among accessions were identified. The thebaine content of the accessions was determined via HPLC analysis. Of the 53 accessions, 21 contained thebaine alkaloid ranging from trace amounts to 2.5% and the rest contained no thebaine. RAPD-PCR markers were used for analysis of accessions at molecular level. A total of 81 of bands generated by 15 successful RAPD-PCR reactions were detected as polymorphic. The polymorphism rate was approximately 85%. The combination of morphological, chemical, and molecular marker analyses resulted in a better classification for the section Oxytona.
  Muhammad AASIM , Sancar Fatih OZCAN , Khalid Mahmood KHAWAR and Sebahattin OZCAN
  Vigna unguiculata is an important food legume crop in the semiarid tropics. It suff ers from a host of agricultural constraints including damage due to diseases and pests. Judicious application of biotechnological methods can lead to considerable improvement in this important crop. Shoot regeneration from unsliced and longitudinally sliced cotyledon node explants obtained from 3-day-old to 5-day-old seedlings grown in vitro was achieved on Murashige and Skoog (MS) medium containing 1.11, 2.22, 3.33, and 4.44 μM benzylaminopurine (BA) supplemented with 1.0 mg/L polyvinylpyrrolidone and 500 mg/L bacteriostatic Augmentin. Callus induction was recorded in all culture media on both explants. Th e shoot regeneration frequency (%) of longitudinally sliced cotyledon node explants was 2-fold to 3-fold higher than that of unsliced cotyledon node explants. A maximum number of 9.92 shoots per longitudinally sliced cotyledon node explant was recorded on MS medium containing 3.33 μM BA. Each increase in the BA concentration of the culture medium proportionally decreased the mean shoot length on both explants. Maximum mean shoot lengths of 2.80 cm on unsliced cotyledon nodes and 3.04 cm on longitudinally sliced cotyledon node explant were recorded on MS medium containing 1.11 μM BA. Regenerated shoots were rooted on MS rooting medium containing 2.45 μM indole-3-butyric acid. In vitro regenerated plants were acclimatised at room temperature in growth rooms, where they produced viable seeds.
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