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Articles by Scott L. Branton
Total Records ( 4 ) for Scott L. Branton
  Spencer A. Leigh , Jeff D. Evans and Scott L. Branton
  Proper vaccine application is required to maximize the results of the vaccination, while maintenance of a homogenous vaccine solution is critical to obtain uniform results. This study was designed to analyze the need for continuous mixing of rehydrated Mycoplasma gallisepticum vaccine solution in order to maintain a homogeneous solution during vaccine application. Commercial F-strain vaccine (AviPro® MG F) was rehydrated and diluted in phosphate buffered saline in accordance with field practices. Dextrose was added to the solution to maintain M. gallisepticum viability without growth during the experiment. The vaccine solution was poured into columns and samples from the static solution were taken from 1, 25 and 50 cm above the base of the column. Samples were taken at 15, 30, 60 and 120 min and compared to a control that was mixed prior to sampling. The results indicate that no significant change in vaccine concentration occurred over the course of the experiment when comparing the mixed control to any of the samples. These results suggest that there is no need for continuous M. gallisepticum vaccine mixing if vaccine is applied within 2 h.
  Brett Weathers , Scott L. Branton , Roymon Jacob , Robert L. Taylor Jr , E. David Peebles and G. Todd Pharr
  Chicken B-cells develop in the bursa of Fabricius. To understand the bursal microenvironment guiding B-cell development, previous studies identified ephrin (Eph) receptor B2 (EphB2) gene transcripts in the embryonic bursa. We hypothesize that the EphB2 receptors and their ligands could initiate contacts between developing B-cells and epithelial cells in the embryonic bursal follicle. To address this hypothesis, additional basic studies were conducted to determine if EphB2 is expressed in the embryonic bursa. Therefore, EphB2 gene expression was examined with reverse transcriptase-PCR and western blotting in the bursa on embryonic days (ED) 15 and 18. The RT-PCR experiments with bursal cDNA amplified transcripts from the EphB2 gene, which was confirmed by nucleotide sequencing. The anti-EphB2 antibody recognized a protein of the expected 120 kDa molecular mass and gave an acceptable signal to noise ratio on whole tissue protein western blots from the ED15 and ED18 bursa. This information can be used to design experiments for determining the cell type (s) expressing the EphB2 protein in the embryonic bursa.
  Nikhil Nuthalapati , Hammed A. Olanrewaju , Scott L. Branton and Gregory T. Pharr.
  Background and Objective: Previous studies have investigated the interaction of different light sources and light intensity. Studies are lacking concerning the effect of different light sources and photoperiods on broiler growth and health. The results reported here are a part of a larger study to evaluate the interaction of different light sources with different photoperiods on the growth of the bursa of fabricius. Materials and Methods: Twelve treatments were designed as a 3×4 factorial arrangement of 4 light sources, incandescent (ICD, control), compact fluorescent light (CFL), light emitting diode (LED) and poultry specific filtered LED (PSF-LED) with 3 different photo periods (short, regular and long). A total of 4 trials were conducted. For each trial a total of 720 broiler chickens were reared under the various light treatments from 8-43 days of age. At 43 days of age, 2 males and 2 females from each treatment group were weighed and then euthanatized for dissection to collect the bursa. The bursas were weighed and the bursa-to-body ratio calculated. Results: The interaction of light source, photoperiod and sex did not have a statistically significant effect on the bursa-to-body weight ratios. Conclusion: The light sources investigated did not have a negative effect on broiler health.
  Balazs Felfoldi , Hui Wang , Nikhil Nuthalapati , Robert L. Taylor, Jr. , Jeffrey D. Evans , Scott L. Branton and Gregory T. Pharr
 

Background and Objective: A previous transcriptomics analysis identified the expression of a pleiotropic cytokine, termed leukocyte cell-derived chemotaxin 2 (LECT2) in the bursa of Fabricius of the chick embryo. However, a role for the LECT2 gene product in the bursal microenvironment is unknown at present. The goal for this research project was to validate the expression of the LECT2 gene at the mRNA and protein level in the chick embryo bursa. Materials and Methods: The LECT2 gene transcript levels were determined by quantitative PCR with RNA derived from embryonic B-cells isolated at two different periods of bursal development. Whole protein extracts from the embryonic bursa were evaluated by mass spectrometry. Results: Expression of the LECT2 gene is significantly higher in B-cells from the early stage of bursal development. Mass spectrometry analysis revealed 9 different peptides from the LECT2 protein in the same embryonic period. Conclusion: The LECT2 gene is expressed at both the mRNA and protein level in the early period of bursa development. We postulate that LECT2 may contribute to B-cell migration into microenvironments established by non-lymphoid cells.

 
 
 
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