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Articles by Sawan Kumar
Total Records ( 2 ) for Sawan Kumar
  Sathyanarayana N. Gummadi and Sawan Kumar
  The effect of temperature and salts on the growth of halotolerant yeast, Debaryomyces nepalensis was studied by growing cells in shake flask on rotary shaker and cell growth was measured. Specific growth rate (μ) of D. nepalensis increased with increase in temperature from 15 to 35 °C and then decreased with increase in temperature beyond 35 °C when grown in presence and absence of different salts. At 40 °C, both NaCl and KCl at 1.0 M concentration enhanced the specific growth rate. D. nepalensis showed synergistic effect on thermal and salt stress when grown at 35-40 °C (0.5 M NaCl) and 30-40 °C (0.5 and 1.0 M KCl). The organism was able to revert its specific growth rate when temperature was shifted from 20 °C to its optimum temperature for growth (30 °C) only in the presence of salts, which was not observed in the absence of salts. However, the same phenomenon was not observed when the temperature was shifted from 40 to 30 °C. Decrease in activation energy was observed for growth at salt concentration beyond 0.5 M of NaCl and KCl.
  Gede Suantika , Magdalena Lenny Situmorang , Pingkan Aditiawati , Abdul Khakim , Shrikumar Suryanarayan , Sri Sailaja Nori , Sawan Kumar and Ferisca Putri
  Background and Objective: Several seaweeds have been reported to contain different bioactive compounds with antimicrobial activity, providing protection against certain infectious diseases in aquaculture production. This study aimed to explore the potential of red seaweed Kappaphycus alvarezii product as an alternative for anti-infective strategy and enhancement of salinity stress tolerance in shrimp (Litopenaeus vannamei) hatchery culture. Materials and Methods: Shrimp post-larvae were fed with Artemia nauplii, either enriched or not enriched with seaweed paste then challenged with Vibrio at day 5 and 8. Comparison of shrimp growth and survival between treatments following salinity stress test and Vibrio challenge were done using one-way analysis of variance analysis. Results: Shrimp fed with seaweed-enriched Artemia resulted in higher survival after Vibrio challenge (90.2±7.0%) compared to shrimp fed with non-enriched Artemia (77.7±3.1%). Shrimp fed with non-enriched Artemia resulted in lower growth after Vibrio challenge (9.65±0.20% b.wt., day–1) compared to the non-challenged group (10.34±0.25% b.wt., day–1). In contrast, there was no difference in the growth of shrimp fed with seaweed-enriched Artemia with or without Vibrio challenge (10.51±0.19 or 10.80±0.28% b.wt., day–1, respectively). The shrimp fed with seaweed-enriched Artemia also obtained a higher survival following salinity stress test (94±2%) compared to shrimp fed with non-enriched Artemia (79±4%). Conclusion: Overall results suggested that red seaweed K. alvarezii by-product enrichment on live feed Artemia for shrimp post-larvae during the hatchery phase can provide protection against V. harveyi infection, improve the growth of shrimp when exposed to pathogenic V. harveyi and also allow higher salinity stress tolerance. Further evaluation on the effect of seaweed by-products dietary supplementation in the nursery and grow-out phases are undoubtedly required, to accurately evaluate the potential of seaweed by-product application as a growth and disease resistance promoting agent in those later phases as well.
 
 
 
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