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Articles by Saurabh Singh
Total Records ( 9 ) for Saurabh Singh
  Bijendra Kumar Singh , Saurabh Singh and S.M. Yadav
  Mangoes account for approximately half of all tropical fruits produced worldwide. India is the largest mango producer accounting for about half of the global mango production. This research attempts to study about the production, area, productivity, disease associated with mango, management and factor which are responsible for the low production of Mango. Mango, a tropical fruit of great economic importance is generally harvested green and then commercialised after a period of storage. Unfortunately, the final quality of mango batches is highly heterogeneous in fruit size as well as in gustatory quality and postharvest behaviour. A large amount of knowledge has been gathered on the effects of the maturity stage at harvest and postharvest conditions on the final quality of mango. Considerably, less attention has been paid to the influence of environmental factors on mango growth, quality traits and postharvest behaviour. The preharvest factors presented here are light, temperature, carbon and water availabilities which can be controlled by various cultural practices such as tree pruning, fruit thinning and irrigation management. Recent advances are also discussed in modelling mango function on the tree according to environmental conditions that combined with experimental studies, can improve our understanding of how these preharvest conditions affect mango growth and quality.
  Saurabh Singh , Asha Sinha and Richa Raaj
  Mungbean seeds genotype HUM-4 and HUM-12 were infested with four dominant fungi viz., Alternaria alternata, Aspergillus niger and Penicillium citrum and Fusarium moniliformae as well as fresh seeds were used to test germination ability of mungbean seeds by using different methods viz., Blotter methods, Multi-pot trey methods, Earthen pot method and Rolled paper towel method. Result showed that all infested seeds were found to be significantly reduced the germination of seeds. In Blotter condition, maximum germination was recorded (93.30%) in genotype HUM-12 followed by (90.35%) in Genotype HUM-4. In paper towel methods, freshly harvested mungbean seeds showed maximum seed germination (90.00%) in genotype HUM-12 followed by (88.67%) in Genotype HUM-4. In multipot trey method, freshly harvested mungbean seed showed maximum seed germination (84.12%) in genotype HUM-12 followed by (83.33%) in Genotype HUM-4. In earthen pot methods freshly harvested mungbean seed showed maximum seed germination (87.30%) in genotype HUM-12 followed by (85.23%) in Genotype HUM-4. Aspergillus niger infested seeds showed lowest germination followed by Penicillium rubrum infested seeds.
  Bhupendra Kumar Singh , Saurabh Singh and D.N. Shukla
  The present study was conducted to investigate the isolation of fungi from Ganga river, Industrial water and Sewage water. Different kinds of fungi were isolated in which Fusarium spp. and Alternaria alternata were found to be dominant. The maximum numbers of fungal species were isolated from sewage water and it was followed by Ganga water and industrial water due to presence of high organic load. Plant pathogenic fungi like Fusarium spp. and Alternaria spp. were found in all water samples. In this way, it may be concluded that, the irrigation of agricultural fields with polluted water not only affects the growth of crop plants but it also increases the chances of various fungal contamination and disease development. The sewage water may be used for irrigation purposes after proper treatment. It will be highly useful, if sewage water diluted with tube well water.
  S.M. Yadav , R.K. Patil , Saurabh Singh , L.P. Balai and Rai Ajay Kumar
  The present investigation on was undertaken in the Department of Plant Pathology, B.A. College of Agriculture, A.A.U., Anand. The Penicillium funiculosum rot of aonla was found a new record in Gujarat and it was identified by Division of Mycology and Plant Pathology, Indian Agricultural Research Institute, New Delhi (ITCC No.: 7046.08). Bio-efficacy of nine phytoextracts at 10% were tested against the mycelial growth and sporulation of Penicillium funiculosum in vitro, among them significantly lowest mycelial growth of P. funiculosum was recorded in neem leaf extract (7.75 mm) showing 89.49% growth inhibition. Further it was also proved most effective in reducing the Penicillium rot severity both in pre- (20.44%) and post-inoculation (20.87%) treatments at 7 day after inoculation, respectively. The next best treatment in tested phytoextracts tulsi and garlic were found both in in vitro and in vivo condition.
  Saurabh Singh , Asha Sinha , Shakshi Singh , Richa Raaj and J. Mishra
  Good quality seed is the prerequisite to agriculture and though of such vital significance very less attention has been paid to their efficient storage and storage losses. The present study was undertaken to screen for isolation and percent incidence of seed borne mycoflora wherein two Mungbean varieties viz. HUM-4 and HUM-12 were screened by standard blotter paper and agar plate methods at the different period of storage. Seeds of Mungbean varieties were obtained from agriculture farm, Banaras Hindu University, Varanasi. The surface sterilization was done by 0.1% mercuric chloride (HgCl2) for 1 min and washed thoroughly with distilled water. Both sterilized and unsterilized seeds were used for detection of seed borne pathogen. A total of 22 different fungi including one bacterial isolate belonging to 12 distinct genera were isolated were from these seed samples. The percentage incidence of different fungi varied with the storage periods. Aspergillus niger recorded at maximum level in 22.33, 43.27, 49.95, 19.98, 22.98 and 39.00% in Agar plate method from fresh, 180 and 360 days period of storage in HUM-4 as well as HUM-12 genotype. Highest incidence of seed borne fungi was recorded in genotype HUM-4 in Agar plate followed by HUM-12 through blotter condition. In all storage period unsterilized seed yielded most number of seed borne fungi as compared to sterilized seed. Result revealed that in all storage period Aspergillus sp. Penicillium sp. Curvularia lunata were recorded highest in HUM-4 through Agar plate method, Fusarium sp. were recorded highest in HUM-4 blotter method and lowest in HUM-12 agar plate methods, Rhizopus Cephaliofhora irregularis, Chaetomium globosum and Cladosporium cladosporoids in HUM-12 under Agar condition. Result showed that maximum number of fungi belonged to Deuteromycotina (68. 98%) followed by Ascomycotina (13. 89%) and Zygomycotina (9. 09%).
  Bhupendra Kumar Singh , Saurabh Singh , Vandana Srivastava and D.N. Shukla
  Water fungi and fungus-like organisms as a biological factor of ecological water systems have significant influence on the environment and its modification. Fresh water samples were collected from region of Ganga River in Varanasi district, Uttar Pradesh, India. Three sampling sites which are known as ghats (stairways to the bank) Assi ghat, Rajendra ghat, Harishchandra ghat) were selected for sample collection. The water samples were examined for fungi by plating method culturing Potato dextrose agar medium. The isolated fungal strains were identified by lactophenol cotton blue staining. Results were found that a total of 23 micoflora were found to be dominant at three place of Ganga River in Varanasi District. In all three places maximum number of fungi was recorded in Assi ghat followed by Harishchandra ghat then Rajendra ghat. Aspergillus flavus and Aspergillus niger showed highest 36.75 and 29.50% frequency at Harishchandra ghat and Rajendra ghat. White sterile mycelium and Trichothecium roseum showed lowest 0.23 and 0.45% frequency in Assi ghat.
  Bijendra Kumar Singh , Saurabh Singh and S.M. Yadav
  Aonla (Emblica officinalis Geartn) king of arid fruits popularly known as “Indian gooseberry” is a small sized minor subtropical fruit that grows widely in North India. India ranks first in the world in area and production. It is considered as “Wonder fruit for health” because of its unique qualities. It is a rich source of vitamin C. It is a perishable fruit and therefore it is necessary to extend its shelf life by adopting good post harvest management practices. Post harvest loses are the major constraints which discourage farmers to go for aonla cultivation. The extension of shelf life may be possible by checking the rate of transpiration, respiration and by checking microbial infection. Different chemicals like waxol, Ca (NO3)2, CCC, carbendazim, GA3, borax, kinetin and packaging materials like nylon net, perforated PE bags, ventilated CFB boxes, gunny bags, wooden crates etc., can be used for prolong the storage life of fruit. Refrigerated storage also helps in enhancing the storage life of fruit. There are so many products like aonla squash, candy, jam and chutney prepared from fruit and it also has the quality to prevent the disease like skin disease, hair falling, blood pressure and some other diseases.
  Saurabh Singh , Bijendra Kumar Singh , S.M. Yadav and A.K. Gupta
  Agriculture provides food for humans, directly and indirectly. As world population is increasing, it is necessary to use the modern technologies such as bio and nanotechnologies in agricultural sciences. Nanotechnology has been defined as relating to materials, systems and processes which operate at a scale of 100 nm or less. Nanotechnology has many applications in all stages of production, processing, storing, packaging and transport of agricultural products. Nanotechnology will revolutionize agriculture and food industry by innovation new techniques such as: Precision farming techniques, enhancing the ability of plants to absorb nutrients, more efficient and targeted use of inputs, disease detection and control diseases, withstand environmental pressures and effective systems for processing, storage and packaging.
  Vasker Bhattacherjee , Kristin H. Horn , Saurabh Singh , Cynthia L. Webb , M. Michele Pisano and Robert M. Greene
  Mutations in each of the transcriptional co-activator genes - CBP, p300, Cited2, Cart1 and Carm1 - result in neural tube defects in mice. The present study thus furnishes a complete and comparative temporal and spatial expression map of CBP/p300 and associated transcriptional co-activators, Cited2, Cart1 and Carm1 during the period of murine neural tube development (embryonic days 8.5 to 10.5). Each co-activator except Cart1 was expressed in the dorsal neural folds on E8.5. Although CBP and p300 are functionally interchangeable in vitro, their respective expression patterns diverge during embryogenesis before neural fold fusion is complete. CBP gene expression was lost from the neural folds by E8.75 and was thereafter weakly expressed in the maxillary region and limb buds, while p300 exhibited strong expression in the first branchial arch, limb bud and telencephalic regions on E9.5. Cart1 exhibited strong expression in the forebrain mesenchyme from E9.0 through E10.5. Although CBP, p300, Carm1 and Cited2 share temporal expression on E8.5, these co-activators have different spatial expression in mesenchyme and/or the neuroepithelium. Nevertheless, co-localization to the dorsal neural folds on E8.5 suggests a functional role in elevation and/or fusion of the neural folds. Target genes, and pathways that promote cranial neural tube fusion that are activated by CBP/p300/Carm1/Cited2/Cart1-containing transcriptional complexes await elucidation.
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