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Articles by Sathyanarayana N. Gummadi
Total Records ( 6 ) for Sathyanarayana N. Gummadi
  Swati Sucharita Dash and Sathyanarayana N. Gummadi
  Pseudomonas sp. NCIM 5235 capable of degrading high concentrations of caffeine has been previously isolated from the soil of coffee plantation area. The isolate was capable of degrading 6.4 g L-1 initial concentration of caffeine at a rate of 0.1 g L-1 h-1. In this study, the physical parameters viz., pH, temperature and shaking speed have been optimized using central composite design. The optimum values of pH, temperature and shaking speed were found to be 7.8, 28°C and 190 rpm, respectively. Under optimized condition of pH, temperature and shaking speed, the rate of degradation of caffeine has been enhanced from 0.18 to 0.29 g L-1 h-1 which is 1.6 fold higher than the normal rate. This is the first report on degradation of high concentration of caffeine at higher rates. Under optimal conditions, the strain has also been found to degrade caffeine at 15 g L-1 initial concentration efficiently within 48 h. This makes Pseudomonas sp. NCIM 5235 an attractive candidate for development of biodecaffeination strategies.
  Swati Sucharita Dash and Sathyanarayana N. Gummadi
  The study was undertaken to investigate the effect of caffeine (1, 3, 7- trimethylxanthine) on growth, morphology and viability of caffeine degrading Pseudomonas sp. and other non caffeine degrading bacterial strains. Growth, morphology and cell viability of the bacterial strains were studied in caffeine medium, minimal medium without caffeine and in minimal medium with caffeine added at log phase of growth. The caffeine degrading Pseudomonas sp. achieved a maximum cell dry weight of 1.1 g L-1 after caffeine addition at log phase of growth without any change in morphology. The growth and viability of E. coli DH5α and other bacterial strains was greatly reduced upon addition of caffeine at log phase of growth. E. coli DH5α strain formed long filamentous cells on caffeine exposure and lysis occurred for other Gram negative bacterial species and Bacillus subtilis. E. coli DH5α transformed with plasmid from caffeine degrading Pseudomonas sp. was found to be tolerant to caffeine. This study shows the susceptibility of non caffeine degrading bacterial strains to caffeine and will be helpful in understanding the basics of the evolution and survival of xenobiotic degrading strains in nature.
  Swati Sucharita Dash and Sathyanarayana N. Gummadi
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  Sathyanarayana N. Gummadi and D. Sunil Kumar
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  Sathyanarayana N. Gummadi and D. Sunil Kumar
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  A. Nagarajan , N. Thirunavukkarasu , T.S. Suryanarayanan and Sathyanarayana N. Gummadi
  L-asparaginase (E.C.3.5.1.1) has been commonly used for the treatment of acute lymphoblastic leukemia in adults and children. It is also used in food industry to reduce acrylamide formation during the preparation of fried food items containing starch at high temperatures. Several microorganisms from the diverse group of bacteria and yeast were reported to be used for L-asparaginase production however, many of the strains also coproduce L-glutaminase which is highly undesirable as it results in cellular stress and neurotoxicity. Thus identification of new sources for the production of glutaminase free L-asparaginase needs to be explored. In this study, we screened endophytic fungi isolated from trees of moist deciduous and semi evergreen forests of the Western Ghats and plants growing in Rono Hills, Arunachal Pradesh, India for the production of glutaminase free L-asparaginase. Using a simple agar plate assay, we found that 33 strains were positive for the L-asparaginase activity among which 19 strains showed glutaminase free L-asparaginase activity. Our results show that: Alternaria sp. endophytic in the leaf of Withania somnifera and growing in the moist deciduous forest of the Western Ghats showed maximum enzyme activity. Optimization of process parameters reveal that maximum L-asparaginase production was observed at 96 h of fermentation and high concentration of glucose in the medium as the carbon source inhibited enzyme production in Alternaria sp. This is the first report on production of glutaminase free L-asparaginase by fungal endophyte Alternaria sp.
 
 
 
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