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| Articles
by
Sarjeet Singh |
Total Records (
1 ) for
Sarjeet Singh |
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Shoor Vir Singh
,
Saurabh Gupta
,
Kundan Kumar Chaubey
,
Krishna Dutta Rawat
,
Naveen Kumar
,
Jagdip Singh Sohal
,
Sarjeet Singh
,
Ruchi Tiwari
,
Sandip Chakraborty
and
Kuldeep Dhama
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Bovine Johne’s
Disease (BJD) is a chronic granulomatous enteritis that affects ruminants worldwide
and is having significant impact on the world economy and has been frequently
reported from farm and farmer’s
herds. An attack of Johne’s disease
in a newly established cattle dairy farm consisting of high yielding Holstein
Friesian (HF) cows in the Alwar district of Rajasthan was investigated for the
first time in India. Since slaughter of cows is prohibited in India therefore
management of bovine JD is critical for the success of dairy industry in the
country and in this aspect the research paper is significant. Out of a total
of 35 fecal samples screened by microscopy, 24 (68.5%) were positive for Mycobacterium
avium subspecies paratuberculosis (MAP). Screening of 26 serum and
23 milk samples by ‘Indigenous
ELISA kit’ employing semi-purified
antigen of native strain (‘S
5’) of MAP, 24 (92.3%) and 14
(60.8%) were positive, respectively. Sensitivity of ‘Indigenous
serum ELISA’ with reference to
fecal microscopy and milk ELISA was 88.2 and 90.0%, respectively. Screening
of blood samples of 14 cows, by specific PCR (IS900), 5 (35.7%) were
positive. Genotyping of PCR positive HF crossbred cows using IS1311 PCR-REA
showed presence of highly pathogenic ‘Indian
Bison type’ genotype. Comparison
of 3 tests (milk ELISA, fecal microscopy and IS900 PCR) with ‘Indigenous
serum ELISA’ revealed substantial
agreement between tests. Study also reported serious economic losses in terms
of productivity (reduced quality and quantity of milk), reduced fertility and
conception, decreased body weight and growth rate which left the farmer economy
devastated due to attack of Johne’s
disease in high yielding cattle herd of HF crossbred cows. |
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