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Articles by Sarder Nasir Uddin
Total Records ( 4 ) for Sarder Nasir Uddin
  Sarder Nasir Uddin , Soubir Titov and Md. Abdul Wadud
  Shoot tips of a traditional table banana [Musa sp. cv. Kanthali (Genome AAB)] of Bangladesh were evaluated for in vitro propagation. Initial surface sterilization (with 0.1% HgCl2 for 12 min) of shoot tips was successful but microbial contamination (mostly bacteria) at the rhizomatous base of the explants were observed within 6-15 days after inoculation which eventually killed 85% inoculated explants. So, for contamination free culture establishment explants were soaked in two broad spectrum antibiotics namely ampicillin and gentamicin. Cent percent contamination free cultures were established by soaking the explants in 400 mg L-1 ampicillin or 200 mg L-1 gentamicin for 1 h. Antibiotic treated explants were found to be full contamination free but failed to regenerate after 3 weeks of culture. But some of them absorbed media for up to 2nd subculture and showed swelling of explants and some color changes from pale white to light/deep green. Finally, a few days after 3rd subculture, no growth of explants was observed and all treated explants eventually started to die. Among the untreated alive explants the best medium for single shoot development was MS + 4.0 mg L-1 BA + 0.5 mg L-1 Kn + 15% CW and average time required for shoot development was 18-21 days. But the regeneration percentage was very low (30%). The best medium for shoot multiplication was MS + 4.0 mg L-1 BA + 2.0 mg L-1 IAA + 15% CW and average time required for production of multiple shoots from single shoot was 40-45 days. Multiplication rate was also too low (40%) and only average 3-4 shoots were formed. Finally, in vitro proliferated shoots produced roots with maximum frequency (90%) in half strength of MS medium fortified with 0.5 mg L-1 IBA.
  SK. Amir Hossain , Sarder Nasir Uddin , Muhammad Sadiqur Rahman , Abdul Wadud and Muhammad Harunuzzaman Khan
  Infectious bursal disease is an acute, highly contagious viral disease of young chickens caused by a double stranded RNA virus named Infectious Bursal Disease Virus (IBDV). Chicken Embryo Fibroblast (CEF) is chicken embryo derived primary cell culture. The present research was undertaken to study the propagation and observation of cytopathic effect of IBDV in chicken embryo fibroblast cells. Chicken Embryo Fibroblast (CEF) is chicken embryo derived primary cell culture. For this purpose, suspected IBDV isolates were collected from the bursas of dead chicken of a particular flock. Local field IBDV isolates was then inoculated in chicken embryo for slight adaptation by several passages. CEF cells was prepared from 9-10 days chicken embryo and transferred in monolayer culture flasks containing maintenance media with 1-2% heat-inactivated fetal calf serum. Adapted IBDV was then inoculated in CEF cell culture for the purpose of propagation. Characteristics clear and consistent Cytopathic Effects (CPEs) were observed on CEF cell culture after 144 h of incubation following infection of IBDV.
  Sarder Nasir Uddin , A.M. Hasan , M.R. Anower , M.A. Salam , M. J. Alam and S. Islam
  Until the advent of genetic engineering, enzyme producers were limited in their ability to produce innovative products for the marketplace. They were constrained to isolate enzymes from organisms approved for the industrial use. The desired characteristics could be enhanced only using classical in lit agenesis techniques. When these methods failed, no alternatives were available. Commercialization depended on incremental yield improvements gained by continuous programs of strain development. The ability to use recombinant DNA (rDNA) techniques has removed many of these barriers. Enzyme producers recognized early the potential for commercialization new products using genetic manipulation. They worked with a wide variety of single-celled organisms that were simpler and thus, easier to understand then the higher orders of plants and animals. The organisms already were well characterized for growth and expression rates. Short life cycles allowed rapid testing. These systems were ideal for genetic manipulation using rDNA techniques. Genetic engineering, combined with an understanding of biocatalysts to predict alterations for enzyme improvements, is revolutionizing the production and use of enzymes in the marketplace. Offering a recombinant produced product represents the culmination of a long and complex effort on the part of a multitude of disciplines: molecular and microbiology, X-ray crystallography, enzymology, protein and organic chemistry, biochemistry, fermentation and formulation engineering, assay chemistry and technical service/applications, marketing, sales. Because of the variety of disciplines required, a critical mass is needed to innovate products successfully and them to market. The continued proliferation of novel enzyme products requires development of core technologies so complex and expensive that they can be justified only rDNA technology must consider regulatory issues, ownership protection and consumer acceptance.
  Abdul Wahab Khan , S. J. Hossain and Sarder Nasir Uddin
  To investigate the antibiotic susceptibility, Vibrio parahaemolyticus were isolated and identified from the sample of shrimps collected from different shrimp processing plants located at Rupsha, Khulna. Among the 27 samples, 10 strains were identified as Vibrio parahaemolyticus. Susceptibility of these Vibrio parahaemolyticus was assayed by disc diffusion method against 11 antibiotics. All these strains were highly sensitive to Tetracycline, Norfloxacin, Gentamicin, Riphampicin and Neomycin, but were resistant to Erythromycin, Penicillin, Ampicillin and Kanamycin. Vibrio parahaemolyticus showed both resistant and susceptible character against Cephalexin and Streptomycin. 7 strains were resistant and 3 were sensitive to Cephalexin on the other hand 8 were resistant and 2 were sensitive to Streptomycin, among the 10 Vibrio parahaemolyticus strains. The antibiotics named Tetracycline, Norfloxacin, Gentamicin, Riphampicin and Neomycin should be the choice to control diseases due to Vibrio parahaemolyticus as well as enteric bacteria.
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