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Articles by Santosh Dhillon
Total Records ( 2 ) for Santosh Dhillon
  Sandeep Deswal , Prasad Minakshi , Koushlesh Ranjan , Santosh Dhillon , Yashpal Singh Malik and Gaya Prasad
  The present study describes characterization of a novel rotavirus (B29) isolated from a buffalo calf causing severe diarrhea. It was confirmed by VP4 and VP7 gene based RT-PCR and in sequence analysis, the VP4 gene showed up to 99.3% sequence identity at nucleotide as well as amino acid levels with P[3] rotavirus genotypes. Similarly, VP7 gene showed a maximum nucleotide and amino acid identity of 99.1 and 98.7%, respectively with G3 genotypes of group A rotavirus (RVA) from several host species and places. The phylogenetic analysis of VP4 gene also revealed close relatedness with other P[3] genotype of rotaviruses from bovine, goat, canine and feline origin. Similarly, VP7 gene revealed close relation with several G3 rotaviruses from bovine and equine origin. The buffalo rotavirus isolate B29 was genotyped as G3P[3]. This is the first report of rotavirus isolate with a G3P[3] genotypic constellation in buffalo calvesfrom this region of India. The detection of buffalo rotavirus of G3P[3] genotypic combination reveals an unexpected epidemiological situation and diversity of bovine rotaviruses in India.
  M.N.R. BAIG , Sapna GREWAL and Santosh DHILLON
  Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa and Blood red, showing maximum similarity (82%). The Jatti-Khatti and King Mandarin were found to be genetically most diverse. The genetic variation between cultivars was quite high and revealed their different origins.
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