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Articles by Sanjeev Kumar Sharma
Total Records ( 2 ) for Sanjeev Kumar Sharma
  Jag Mohan , Sanjeev Kumar Sharma , Gautham Kolluri , Jagbir Singh Tyagi and Jag Mohan Kataria
  This study was conducted to identify the suitable diluent for short-term preservation (0, 24 and 48 h) of Guinea fowl (pearl variety) semen. Semen samples were collected and diluted with normal saline, CARI, BPSE and Lake’s semen diluents and kept at 6°C for storage. No significant difference was found in sperm motility among the different diluents at 0 h (freshly ejaculated) storage period. A significant (p<0.05) reduction was found in sperm motility among all the diluents at 24 h storage period. Subsequently, there was no sperm motility was recorded in normal saline diluted semen samples at 48 h storage period. While a reduction (p<0.05) in sperm motility was recorded in rest of the three semen diluents. The mean values for fertility with freshly ejaculated semen during 2-12 days of fertile period were recorded as 66.27±4.08, 78.90±3.08, 64.90±4.87 and 67.99±6.36% whereas, after 24 h storage of semen, fertility reduced drastically such as: 0, 41.97±7.27, 36.09±8.72 and 30.90±7.12% in normal saline, CARI, BPSE and Lake’s semen diluent, respectively. No fertility was noticed at 48 h storage in any semen diluent. Irrespective of the storage period and diluents, CARI semen diluent expressed better fertility than others in Guinea fowl.
  Azhar Rashid , Sanjeev Kumar Sharma , Jagbir Singh Tyagi , Jag Mohan , Raj Narayan , Gautham Kolluri and Ram Pratap Singh
  Background and Objective: Elevated Incubation Temperature (EIT) plays an important role in the regulation of nutrient transporter gene (NTG) expression. Information on NTG expression in Japanese quail is scarce. Thus, an attempt was made to understand the effects of EIT on the mRNA expression of NTG in CARI-UTTAM and CARI-PEARL varieties of Japanese quail. Materials and Methods: Eggs of first treatment group (control) were subjected to 37.5°C throughout the entire incubation period (17 days). Eggs in 2nd and 3rd treatment group were incubated at 37.5°C for initial 10 days and there after at 38.5 and 39.5°C, respectively. Eight chicks (4 from each variety) from each treatment group were randomly sacrificed on Day Of Hatch (DOH), 3rd, 7th and 10th day of age to study mRNA expression of NTG. Results: Expression of SGLT1 gene was significantly up regulated on all the studied days except on DOH at 38.5°C and on 3rd day at 39.5°C in CARI-PEARL variety. Expression of GLUT5 gene was up regulated on all days except on DOH at 38.5°C in CARI-UTTAM variety. PepT1 gene expression was up regulated on 3rd day of age and over expression of EAAT3 gene was significant on 3rd and 10th day in both the varieties at elevated incubation temperatures. At incubation temperature 38.5°C UTTAM variety showed over expression for SGLT1 gene at DOH and 3rd day, for GLUT5 and PepT1 gene at 10th day and for EAAT3 gene at 7th and 10th day post-hatch in comparison to PEARL variety. At 39.5°C higher expression is observed on 7th post-hatch and DOH for SGLT1, 7th and 10th day post-hatch for GLUT5 and PepT1 and 3rd and 7th day post-hatch for EAAT3 gene. Conclusion: A significant up regulation in the mRNA expression of most of the studied nutrient transporter genes were reported at elevated incubation temperatures. In nut shell, it may be concluded that higher relative fold expression of most of the nutrient transporter genes in quail incubated at elevated temperatures may indicate higher susceptibility of jejunum to this temperature warranting higher fold expression as an adaptation mechanism.
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