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Articles by Sandeep Suryan
Total Records ( 2 ) for Sandeep Suryan
  Mahadevan M. Sundar , Nagananda G.S. , Arijit Das , Sourav Bhattacharya and Sandeep Suryan
  Bacteriophages have been found to be effective against a wide variety of pathogenic bacteria as they are highly host specific. The present study describes the isolation of bacteriophages effective against few human pathogens such as Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa. A total of five isolates of bacteria were obtained from the sewage water, sampled from the sewage treatment plant located at Jinke Park, Bangalore, India. Based upon the colony morphology, biochemical characterization and growth on selective media, the isolates were identified as Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella species and Shigella species. Out of the five different isolates three were sensitive to bacteriophages. The sensitive cultures belonged to the genera Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa. The phage filtrates, when spotted onto the lawn cultures of the respective host bacterium, resulted in the development of clear zones indicating the presence of lytic bacteriophages against the host bacteria. It was also found that each of the phages for E. coli and Salmonella typhi was only able to infect its original host bacterium, whereas, the phage for Pseudomonas aeruginosa was able to infect both Pseudomonas and E. coli. Studies of the morphology and characterization of these phages are currently being conducted. These isolated phages may hold a lot of promise as the first choice of prophylaxis (Phage Therapy) against nosocomial and secondary infections by deadly multi-drug resistant bacteria in the near future.
  Mahdi Eskandarian Boroujeni , Arijit Das , K. Prashanthi , Sandeep Suryan and Sourav Bhattacharya
  Actinomycetes constitute an economically important class of bacteria for their ability to produce various degradative enzymes and secondary metabolites. The present study was conducted with the objectives to isolate some soil actinomycetes, screen them for production of hydrolytic enzymes and characterize them. A total of 30 isolates of actinomycetes were obtained from six soil samples collected from Kuruva Island and the banks of Pookot Lake from Wayanad district in Kerala, India. Screening for enzyme production among 18 randomly selected isolates revealed that 13 of them were able to synthesize either cellulase, pectinase and/or xylanase. The results indicated that 44.4% of the total isolates produced all the three enzymes. Based on morphological and biochemical characterization, the active isolates were found to belong to the genus Streptomyces. The relatedness between the 13 active isolates was determined by RAPD fingerprinting using 20 different primers. Only amplification with primer OPAB-9 resulted in good scorable bands and revealed a prominent band at 600 bp in 54% of the isolates. The dendrogram showed that the active isolates fell into one super cluster which further consisted of three clusters and one out group. The presence of identical cultural characteristics was detected in most of the clusters. With respect to the enzyme activity, the similarity was only noticed among majority of the members of cluster 1. This molecular study may be employed as an effective tool to characterize streptomycete isolates capable of producing various industrially important enzymes.
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