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Articles by Sanaa O. Yagoub
Total Records ( 7 ) for Sanaa O. Yagoub
  Fardous M. Bellow , Sanaa O. Yagoub and Ibtisam E.M. El Zubeir
  In the present study the effect and survival of Bacillus spp. (Bacillus subtilis and B. cereus) in milk samples that were collected from Khartoum, Khartoum North and Omduraman were studied. In order to evaluate the degree of spoilage by those organisms, sterile milk sample were inoculated by 3.1x103 cfu mL-1 of B. cereus and 3.5x103 cfu mL-1 B. subtilis and stored at 7, 12, 21, 37 and 45°C. The milk samples were investigated daily for total bacterial counts, fat %, protein % and acidity. The present results revealed that Bacillus spp. inoculated in milk samples showed significant differences for protein, fat, acidity and bacterial count (p<0.05) at different storage periods and temperatures. Moreover the milk samples showed a shelf life of more than 5 days at 7 and 12°C. However, the milk samples that stored at 21, 37 and 45°C recorded a shelf life of 1-4 days.
  Sanaa Kabashi El-Toum and Sanaa O. Yagoub
  The antimicrobial activity of three types of Bees honey (Sidir, Sunflower and Sunut) were examined against (Staphylococcus aureus, Escherichia coli, Klebsiella aerugenes, Pseudomonas aeruginosa and Candida albicans) at concentration 100, 75, 50 and 25%. Sidir honey showed antimicrobial activity against Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa and Candida albicans, the zone of inhibition range between (9-50 mm), while the E. coli showed markedly resistance towards all concentrations used. Sunflower honey showed markedly sensitivity towards E. coli, Staphylococcus aureus and Klebsiella aerogenes, the inhibitions zone were between (15-50 mm), while both Candida albicans and Pseudomonas aeruginosa showed clearly resistance towards all concentrations used. Sunut honey showed antimicrobial activity toward Staphylococcus aureus, E. coli, Klebsiella aerogenes and Candida albicans, the inhibition zone range between (10-42 mm), while Pseudomonas aeruginosa showed resistance toward all concentrations used. The antibacterial activity was compared with Gentamicin and Tetracycline, while the antifungal activity was compared with Nystatin.
  Rasha S. Ahmed and Sanaa O. Yagoub
  Petroleum ether, methanol and water extracts of Psidium guajava leaves were tested to determine their possible antimicrobial activity against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative bacteria (E. coli and Salmonella typhi) and fungi (Aspergillus niger and Candida albicans). Each extract was used in concentration of 100, 50, 25 and 10%. Gram-positive and Gram-negative bacteria showed susceptibility toward all extracts, the zones of their inhibition ranged between (13-28 mm). Aspergillus niger showed complete resistance toward all extracts at all concentrations while Candida albicans was inhibited by water extract, all concentrations of petroleum ether extract and only concentration at 10% of methanol extract, the zones of inhibition ranged between (13-20 mm).
  Nasserin Hashim , Ali Hassabo and Sanaa O. Yagoub
  The aim of present study was to investigate the seroprevalence of brucellosis among cattle and cattle-owners at selected area of Khartoum state. This research was carried out in Gubul Awliaa area farms, out of 175 unvaccinated cattle 84 (48%) were shown positive agglutination reaction towards Br. melitensis while 44 (26.4%) showed positive reaction toward Br. abortus. Mix infection was also reported. The highest incidence of infected animals and mix infection were recorded in cows with more than 10 years old. Cows milking between 6-10 times showed highest incidence of the infection. 33.1% of positive Br. melitensis cattle showed titre of 160 while 24.6% of the positive Br. abortus showed titre of 160. The Ring Milk Test (RMT) was positive in 80% of examined milk samples. On the other hand 40% from examined milker-sera showed positive agglutination results with Br. melitensis with titre of 160.
  Sanaa O. Yagoub and Rawda Yousif Ahmed
  This study was aimed to evaluate the microbial quality of drinking water distributed at Khartoum state- the capital of the Sudan. Water distributed at piped system was investigated using two different standard methods (MPN and chromogenic media- based techniques), 47.5-90% showed positive isolation of bacteria. The results revealed isolation of faecal coliform (E. coli), coliform group (Klebsiella sp., Citrobacter sp., Enteriobacter sp.), some pathogenic and potential pathogenic bacteria (Staphylococcus aureus, Salmonella sp., Yersienia enteriocolitica, Proteus sp., Bacillus sp. and Pseudomonas aeruginosa) were isolated. Other bacteria with significant importance were detected. The quality of drinking water, types and number of isolated bacteria were evaluated and discussed according to seasons and locations.
  Sanaa O. Yagoub , Fardous A. Bellow and Ibtisam E.M. El Zubeir
  The effect of incubation temperature and storage period on inoculated milk by Pseudomonas aeruginosa which well known as milk-spoilage microorganism was studied. Sterile milk sample were inoculated by 108-109 cfu mL-1 P. aeruginosa and stored at 0, 7, 12, 21, 37 and 45°C. The chemical analysis was conducted daily for all stored milk samples to investigate the effect of bacteria on milk constituents (fat%, protein% and acidity) and total bacterial count. The present results showed that milk which inoculated with P. aeruginosa and stored at different storage periods and temperatures, showed variations for fat, protein, acidity and bacterial count. Moreover, the inoculated milk samples showed a shelf life of 1-2 days at 37 and 21°C. However, the milk samples stored at 0, 7 and 12°C showed shelf life that ranged between 4-9 days. The present study concluded that the number of psychrotrophic bacteria significantly affected by both storage period and incubation temperature.
  Sanaa O. Yagoub
  The petroleum ether, ethanol and aqueous extracts of the Tamarindus indica and Adansonia digitata were screened for the presence of possible anti-microbial activity using the cup plate agar diffusion method. They were tested against Escherichia coli that isolated from urine and water sources. Each extract was used in concentration of 100, 75, 50 and 25%. E. coli isolated from clinical urine samples showed more susceptibility toward both plant extracts. The ethanol extract of both plants was more effective than petroleum ether and water extracts. Ethanol extract showed variation in the antimicrobial activity toward E. coli that isolated from water and clinical sources, the zones of their inhibition ranged between (15-60 mm). On the other hand, the petroleum ether extract of Adansonia digitata was found to be inactive against all tested organisms. The susceptibility of the microorganisms to the extracts of these plants was compared with each other and with selected antibiotics. Ethanol extract of Tamaridus indica have more powerful antibacterial activity compared with all antibiotics. The antimicrobial activities of these plants were discussed according to their phytochemical components.
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