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Articles by Samy Ali Hussein
Total Records ( 4 ) for Samy Ali Hussein
  Samy Ali Hussein , Omayma A. Ragab and Mohammed A. El-Eshmawy
  The immunosuppressive drug cyclosporine A (CsA) has been successfully used in several diseases with immunological basis and in transplant patients. Nephrotoxicity is the main secondary effect of CsA treatment. The present study was designed to investigate the possible protective effect of dietary fish oil (F.O.) on CsA-induced nephrotoxicity in rats. Eighty male rats were divided into four equal groups. Group 1: Rats received no drugs and served as control, Group 2: Normal rats were treated with (dietary fish oil) omega-3 fatty acids 270 mg kg-1 b.wt. oral dose daily, Group 3: Rats treated with CsA (25 mg kg-1 b.wt., orally for 21 days) to induce nephrotoxicity, Groups 4: Rats received dietary fish oil for 21 days before, 21 days concurrently during CsA administration and 21 days later after nephrotoxicity induction. Blood samples for serum separation and kidney tissue specimens were collected three times at weekly interval from the last dose of CsA administration. Serum glucose, total Protein, albumin, lipid profile (total cholesterol, triacylglycerols and phospholipids), renal function tests (urea, uric acid and creatinine), electrolytes (sodium and potassium), inorganic phosphorus and haptoglobin levels, lactate dehydrogenase (LDH) and Gamma Glutamyl Transferase (GGT) activities were determined. Moreover, kidney tissue malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), total antioxidant capacity (TAO) levels, antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (Gpx) activities were also determined. The results revealed that, CsA-induced nephrotoxicity caused significant increase in serum glucose, renal functions tests, haptoglobin, lipid profiles and serum marker enzymes (LDH and GGT) with significant decrease in serum total protein, albumin and electrolytes concentrations which were reversed upon treatment with dietary fish oil. Also, CsA administration induced significant elevation in lipid peroxidation (MDA) along with significant decrease in antioxidant enzyme activities, non enzymatic antioxidant, total antioxidant capacity and nitric oxide level in the rat kidney. Meanwhile, Dietary fish oil administration improved renal function, by bringing about a significant decrease in peroxidative levels and increase in antioxidant status. These results indicate the renoprotective potential and usefulness of dietary fish oil, as an excellent source of antioxidants, in modulating CsA-induced nephrotoxicity.
  Samy Ali Hussein , Omnia M. Abd El-Hamid and Haidy S. Hemdan
  The high-fructose diet induces insulin resistance, hyperinsulinaemia, hyperglycemia, alterations in lipid metabolism and oxidative stress in rat tissues which Oxidative stress plays a vital role in pathology associated with insulin resistance. The present study was aimed to explore the effect of L-carnitine (CAR) on insulin resistance, inflammation, oxidative stress, antioxidant status and lipid metabolism in male rats fed with high fructose diet. Insulin resistance was induced by feeding high fructose diet (60 g/100 g). Sixty male albino rats were divided into four groups containing 15 rats each. Group I: (Control group) rats received the control diets. Group II: (fructose-fed group) rats received fructose-enriched diet (60 g/100 g diet). Group III: (fructose+CAR group) animals received high fructose diet and were administered CAR (300 mg kg-1 b.wt. day-1, orally). Group IV: (control+CAR group) rats received the control diet and were administered CAR. After 45 and 60 days of treatment blood samples and liver tissue were collected for determination of serum glucose, insulin, insulin resistance, pyruvate, lactate, leptin, total cholesterol, triacylglycerols, phospholipids, free fatty acids, sialic acid, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and nitric oxide in addition to L-malondialdehyde (L-MDA). Moreover, antioxidant enzymes (SOD,CAT and GPx) in liver tissues were also determined. The obtained results revealed that, high fructose diet induce a significant increased in serum glucose, insulin, insulin resistance, lipid profiles, pyruvate, lactate, lipten, TNF-α, IL-6, sialic acid, MDA and nitric oxide concentrations and decrease serum phospholipids with marked reduction in CAT, SOD and GpX activities in liver tissues compared to rats fed normal diet. L-carnitine treatment to high fructose fed rats reduced the effects of fructose and associated with significant normalization of all serum parameters level and was able to improve dyslipidemia, inflammation and insulin resistance, attenuated the increased MDA and enhanced antioxidant status in liver tissues. These results suggest that, L-carnitine is effective in improving the high fructose induced oxidative stress, inflammation and insulin resistance in male rats. Also, the administration of of L-carnitine to rats fed a high fructose diet prevents the development of oxidative stress and its associated complications include hyperglycemia, hyperinsulinemia and dyslipidemia.
  Samy Ali Hussein , Omayma A. Ragab and Mohammed A. El-Eshmawy
  Cyclosporine A (CsA) is a potent and effective immunosuppressive agent, but its use is frequently accompanied by severe renal toxicity. CsA-induced nephrotoxicity results from increased production of free radical species in the kidney.The present study was designed to investigate the possible protective effect of Green Tea Extract (GTE) on CsA-induced nephrotoxicity in rats. Eighty male rats were divided into four equal groups. Group 1: Rats received no drugs and served as control, Group 2: Normal rats were treated with green tea extract (3% w/v) orally, Group 3: Rats treated with CsA (25 mg kg-1 b.wt., orally for 21 days) to induce nephrotoxicity, Group 4: Rats received green tea extract for 21 days before, 21 days concurrently during CsA administration and 21 days later after nephrotoxicity induction. Blood samples for serum separation and kidney tissue specimens were collected three times at weekly interval from the last dose of CsA administration. Serum glucose, total Protein, albumin, lipid profile (total cholesterol, triacylglycerols and phospholipids), renal function tests (urea, uric acid and creatinine), electrolytes (sodium and potassium), inorganic phosphorus and haptoglobin levels, lactate dehydrogenase (LDH) and Gamma Glutamyl Transferase (GGT) activities were determined. Moreover, kidney tissue malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), Total Antioxidant Capacitys (TAO) levels, antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were also determined. The results revealed that, CsA-induced nephrotoxicity caused significant increase in serum glucose, renal functions tests, haptoglobin, lipid profiles and serum marker enzymes (LDH and GGT) with significant decrease in serum total protein, albumin and electrolytes concentrations which were reversed upon treatment with green tea extract. Also, CsA administration induced significant elevation in lipid peroxidation (MDA) along with significant decrease in antioxidant enzyme activities, non enzymatic antioxidant, total antioxidant capacity and nitric oxide level in the rat kidney. Meanwhile, Green tea extract administration improved renal function, by bringing about a significant decrease in peroxidative levels and increase in antioxidant status. These results indicate the renoprotective potential and usefulness of green tea extract, as an excellent source of antioxidants, in modulating CsA-induced nephrotoxicity.
  Samy Ali Hussein , Omnia , M. Abd El-Hamid and Ahmad M. Sabry Fayed
  Cadmium (Cd) is a well-known human carcinogen and a potent nephrotoxin. The present study was carried to evaluate the protective effects of alpha-lipoic acid and melatonin against cadmium (Cd) induced oxidative stress to erythrocytes in rats. One hundred male albino rats were divided into five groups containing 20 rats each. Group I: (control) administered distilled water. Group II: (Cadmium exposed group) received cadmium chloride (4.4 mg kg-1 b.wt. of 1/20th of LD50) orally and once per day over a period of 10 weeks. Group III: (Cadmium+Alpha-lipoic acid treated group) received cadmium chloride (4.4 mg kg-1 b.wt.) and treated daily with alpha-lipoic acid (54 mg kg-1 b.wt./i.p). Group IV: (Cadmium+Melatonin treated group) received cadmium chloride (4.4 mg kg-1 b.wt.) and treated daily with melatonin (10 mg kg-1 b.wt./orally). Group V: (Cadmium+alpha-lipoic acid and melatonin treated group). Heparinized blood used for Glucose-6-phosphate dehydrogenase and hemoglobin determination. Plasma used for determination of Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST) and Gamma-glutamyltransferase (γ-GT) activities and urea, creatinine, total cholesterol and phospholipids concentrations. Moreover, erythrocyte hemolysate were processed for the determination of L-Malondialdhyde (L-MDA), Catalase (CAT), Superoxide Dismutase (SOD), Glutathione-s-transferaseand (GST) and reduced Glutathione (GSH). Also, liver and kidney specimens were excised for histopathological examination and for cadmium residues determination. The obtained results revealed that, a significant increase in plasma ALT , AST and GGT activities, urea, creatinine, total cholesterol and phospholipids concentrations and erythrocyte L-MDA level, SOD activity ,Liver and kidney cadmium residue were observed in cadmium intoxicated rats. However, administration of alpha-lipoic acid, melatonin and their combination in cadmium intoxicated rats exhibited a significant decreased in all mentioned parameters. On the other hand, a significant decreased in erythrocyte CAT, GST and G-6-PDH activities, GSH and hemoglobin concentrations were observed in cadmium intoxicated rats. Meanwhile, alpha-lipoic acid and melatonin administrations alone and in combination in cadmium intoxicated rats resulted in significant increase in all mentioned parameters. The histopathological studies in the liver and kidney of rats also supported that alpha-lipoic acid and melatonin markedly reduced the Cd induced pathological changes and preserved the normal histological architecture of the liver and kidney tissues.It could be concluded that, the potential of alpha-lipoic acid and melatonin as a powerful agents and may be useful as an antioxidants in combating free radical-induced oxidative stress and tissue injury that is a result of cadmium toxicity. Also, these compounds have a protective antioxidant effect and could be also applicable as a cytoprotective against oxidative stress of tissue damage mediated by heavy metals intoxication.
 
 
 
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