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Articles by Samir Kumar Biswas
Total Records ( 2 ) for Samir Kumar Biswas
  Kahkashan Arzoo , Samir Kumar Biswas and Mohd. Rajik
  The potentiality of different plant extracts like bark of Eucalyptus lanceolotus and Terminalia arjuna, tubers of Cyperus rotundus, leaves of Withania somnifera, Azadirachta indica, Datura stramonium, Acacia arabica, Cymbopogon flexuosus and Parthenium hysterophorus, cloves of Allium sativum, bulb of Allium cepa, fruits of Emblica officinalis and rhizome of Zingiber officinale as inducers were assessed on physiological and biochemical activities in tomato against fusarim wilt caused by Fusarium oxysporum f. sp. lycopersici and the results showed that pre-application of inducers provided protection to the tomato plant and reduced the disease intensity. The minimum disease intensity (8.93%) was reported from garlic extract treated plant whereas, in case of control-I it was 96.12%. Treatment with plant extracts as inducers prior to challenge inoculation sensitized the seedlings to produce increased levels of soluble protein. The maximum increase in protein content was found in garlic extract treated seedlings (32.62 mg g-1) after 15 days of pathogen inoculation. A high content of phenols which are indicators of first stage of defence mechanism, was also recorded in treated leaves with maximum in garlic extract treatment representing 2.28 mg g-1 of fresh leaves against 1.52 mg g-1 of fresh leaves in control-II after 15 days of pathogen inoculation. The soluble protein content (r = -0.5995) and total phenol content (r = -0.5313) both showed a negative correlation with disease incidence. Apart from inducing effect in plant defences, plant extracts have also some direct effect on growth and development of the pathogen. Protein profiling by SDS-PAGE revealed that one new protein is synthesized due to effect of inducers that might be responsible for disease.
  Rakesh Kumar , Samir Kumar Biswas , Virendra Kumar , Kishan Lal , Upesh Kumar and Vallabhaneni Tilak Chowdary
  Background and Objective: Induced resistance is an innovative strategy to overcome production problems of the most serious and destructive late blight [Phytophthora infestans] (P. infestans) diseases in potato, causes great Irish famine in 1845. Biochemical changes are associated with induced resistance. Therefore, the study was undertaken to find out the induction of defense enzymes using inorganic chemicals as inducer in induced resistance against late blight of potato. Materials and Methods: The experiment was conducted at Wire house complex at Department of Plant Pathology during 2012-2015 using inorganic chemicals viz. salicylic acid, indole acetic acid, di-potassium hydrogen orthophosphate, hydrogen peroxide, calcium chloride, ferric chloride and metalaxyl as inducers in induced resistance against the disease. The activity of defense enzymes like peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) in potato plants after treatment with different inorganic chemical as inducers followed by inoculation of pathogen was measured at 2, 4, 6, 8 and 10 days separately according to the standard procedure developed by various scientists. Correlation coefficients (r) between defense enzymes and disease severity were calculated by standard statistical calculation. Results: All the treatments were able to reduce the disease severity with the lowest in salicylic acid treated plant, representing, the value 12.55-20.72% against 46.35-65.35% in case of control. Biochemical analysis revealed that increased content of defense enzymes like POD, PPO and PAL were found in all treated plants but the maximum in salicylic acid treated leaves at different days of interval. Correlation coefficient analysis also revealed that there was negative correlation r = (-0.886, -0.986 and -0.985),(-0.936, -0.985 and -0.986),(-0.945, -0.872 and -0.972) between disease severity and defense enzymes POD, PPO and PAL respectively at 2, 6 and 10 days of treatments. Conclusion: Pre-foliar sprayed with inorganic chemicals stimulate to synthesize the increase content of peroxidase, PPO and PAL which might be provided protection of potato plants against P. infestans. Salicylic acid can be used as inducers in induced resistance in potato against late blight.
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