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Articles by Saleha Abdul Aziz
Total Records ( 2 ) for Saleha Abdul Aziz
  Yaqub Ahmed Geidam , Zunita Zakaria , Saleha Abdul Aziz , Siti Khairani Bejo , Jalila Abu and Sharina Omar
  Prevalence of multidrug resistant bacteria in apparently healthy chickens from 3 selected poultry farms in Selangor area of Malaysia was investigated. Conventional isolation techniques such as growth on selective media, gram staining and biochemical tests were utilised for the identification of the different bacterial isolates. Antimicrobial sensitivity test was monitored with the disc diffusion assay against 12 antimicrobial agents. A total of 96 Staphylococcus aureus, 48 E. coli, 7 Pasteurella sp. and 6 Salmonella sp. were isolated. All E. coli and Salmonella spp. isolates were multidrug resistant while 77.2% of Staphylococcus aureus and 71.5% of Pasteurella sp. isolates were multidrug resistant. The study further revealed highest resistance to tetracycline while cephalothin as the best drug of choice for treatment of infections caused by the isolates in the study area. Since not only chickens are at risk, this study recommends urgent intervention by regulatory agencies to limit the emergence and spread of these bacteria as well as prudent use of antibacterial agents among farmers in Malaysia.
  Ali Abuseliana , Hassan Daud , Saleha Abdul Aziz , Siti Khairani Bejo and Milud Alsaid
  Streptococcal infection was reported to cause significant mortality and high economical loss in freshwater and saltwater fish species including tilapia species, worldwide. Recently, few disease outbreaks affecting cultured red tilapia in Selangor state, Malaysia was investigated. Affected fish showed loss of appetite, serpentine swimming and exophthalmia. Sick and healthy appeared fish were clinically examined and samples from brain, liver, spleen and kidney were collected for agent isolation. All isolates were gram-positive, oxidase-negative, catalase-negative, β-haemolytic cocci and were characterized as a Group B Streptococcus agalactiae (GBS) using commercial identification kits (Streptococcal grouping Kit, RapID™ STR System and BBL Crystal GP ID Kit). The isolates were sensitive to amoxicillin, ampicillin, erythromycin, chloramphenicol, linomycin, rifampicin, vancomycin, gentamicin, sulfamethoxazole + trimethoprime and tetracycline. In contrast, they were resistant to neomycin, amikacin, kanamycin and streptomycin. Specific Polymerase Chain Reaction (PCR) and 16S rRNA sequencing technique results confirmed the isolates as a GBS. Results of this current study indicated that the Streptococcus agalactiae infection started to spread and warrants focusing on ways to prevent and control the disease before it become endemic.
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