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Articles by Sakulrat Rattanakiat
Total Records ( 4 ) for Sakulrat Rattanakiat
  Pawitra Pulbutr , Wanida Caichompoo , Pilanthana Lertsatitthanakorn , Methin Phadungkit and Sakulrat Rattanakiat
  The essential oil from the fruits of Amomum uliginosum K.D. Koenig has been commonly used in Thai herbal formula for the treatment of gastrointestinal diseases. However, the biological activity of the essential oil obtained from A. uliginosum fruits has never been reported. This study was aimed to investigate the pharmacological activities and cytotoxic effect of the essential oil derived from the fruit of A. uliginosum. The essential oil derived from the authentic fruits and the commercial fruits were prepared by distillation with the yield of 2.90±0.00 and 2.90±0.30% v/w, respectively. The chemical constituents of the essential oil were analyzed by GC-MS. Twenty one and 13 chemical compounds were found in the essential oil derived from the authentic and commercial fruits, respectively. The major chemical compound presented in the essential oil from both sources was bornyl acetate. The essential oil exhibited an antibacterial activity against gastrointestinal disorder-related bacteria with the lowest MIC of 75 μL mL-1 against E. coli O157:H7 DMST 12743 and S. aureus DMST 8013. The essential oil exerted the cytotoxic effect on 3T3 fibroblasts with the IC50 of 0.0725±0.0045 μL mL-1. The strong antimutagenic effect in Salmonella typhimurium TA 98 was found at the concentration of 3.75 mL plate-1. These results scientifically confirmed the use of the essential oil derived from A. uliginosum for the medicinal purposes. Additionally, its potential application for the use as cancer chemoprevention is also indicated from this study.
  Pawitra Pulbutr , Sakulrat Rattanakiat , Wanwisa Khunawattanakul , Kritsanee Saramunee and Bunleu Sungthong
  Background and Objective: Streptococcus mutans is a major causative pathogen of dental caries, which is a multifactorial oral infection resulting in progressive tooth destruction. Several medicinal plants, including Morus alba (mulberry tree) have been used in folk medicine for the treatment and prevention of dental caries. The objectives of this study were to evaluate the antibacterial and anti-cariogenic activities of the M. alba leaf extracts against S. mutans and to develop a chewing gum containing the extract. Materials and Methods: The MIC and MBC were determined by using broth dilution and drop plate methods, respectively. Biofilm formation and glycolytic pH drop assays were performed to evaluate the anti-cariogenic activity. High-performance liquid chromatography was used for phytochemical analysis of the extracts. The chewing gum containing the M. alba leaf ethanolic extract was prepared by varying different types and concentrations of ingredients. The freeze-thaw cycle testing was conducted to determine the stability of the developed chewing gum. Results: The ethanolic and aqueous M. alba leaf extracts possessed antibacterial activity against S. mutans with the MICs of 0.14 and 5 mg mL–1, respectively. The ethanolic extract at the sub-MICs significantly inhibited both acid production and biofilm formation of S. mutans. The phytochemical analysis showed that the concentrations of chlorogenic acid and rutin in the ethanolic extract were significantly higher than those in the aqueous extract. Two satisfactory chewing gum formulas with good stability, gum A and B, were obtained. The gum B formula was found to have the better performance. Conclusion: The results from this study demonstrated the anti-cariogenic role of M. alba leaves even at the low concentration of sub-MICs. The prepared chewing gum B formula can potentially be further developed as a medicated chewing gum for the prevention of dental caries in the near future.
  Pawitra Pulbutr , Sakulrat Rattanakiat , Natnicha Phetsaardeiam , Patcharapa Modtaku , Rattazart Denchai , Achida Jaruchotikamol and Wanwisa Khunawattanakul
  Background and Objective: Streptococcus mutans is a dominant causative pathogen of dental caries, which is a major oral health problem affecting million people worldwide. Derris reticulata is a medicinal plant possessing antimicrobial activity against several Gram-positive pathogenic bacteria. None the less, its effects on growth and cariogenic properties of S. mutans has not been clearly established. This study aimed to investigate the antibacterial and anti cariogenic activities of the D. reticulata ethanolic stem extract. Materials and Methods: The TLC analysis was performed to authenticate the D. reticulata sample. Minimum inhibition concentration and minimum bactericidal concentration were determined by using broth dilution and drop plate methods, respectively. Sucrose dependent and sucrose independent-adherences, biofilm formation and glycolytic pH drop assays were performed to evaluate the anticariogenic activity. Results: The ethanolic stem extract of D. reticulata possessed the antibacterial activity against S. mutans with the MIC and MBC of 0.875±0.250 and 1.750±0.500 mg mL–1, respectively. The extract at the lower concentrations of sub-MIC also had significant inhibitory actions against the cariogenic properties of S. mutans, including surface adherence, biofilm formation and glycolytic acid production. Conclusion: The D. reticulata stem extract had a substantial anticariogenic activities and thus potentially be developed as an oral health care product for dental caries prevention in the near future.
  Sakulrat Rattanakiat , Kanladar Kaewchang , Sofia Thongsang , Achida Jaruchotikamol and Pawitra Pulbutr
  Background and Objective: Antibacterial resistance is one of the top global public health problems. The use of natural substances, which can enhance the antibacterial activity of currently used medications, is a promising alternative to oppose antibacterial resistance. The pharmacological activities of lupinifolin, a prenylated flavanone isolated from stems of Derris reticulata Craib., against growth and biofilm formation of Streptococcus mutans and Staphylococcus aureus have been previously documented. Nonetheless, interactions between lupinifolin and other antibacterial agents have not been determined. This study aimed to investigate the effects of lupinifolin in combinations with some antibacterial agents, specifically ampicillin, cloxacillin or vancomycin, against S. mutans, Methicillin-Sensitive S. aureus (MSSA) and Methicillin-Resistant S. aureus (MRSA). Materials and Methods: The checkerboard assay was performed to determine the antibacterial activity of lupinifolin plus the testing antibacterial agents. The Fractional Inhibitory Concentration Index (FICI) was calculated to indicate the interaction between lupinifolin and the antibacterial agent tested. Results: Lupinifolin exerted the synergistic activity when using in combination with ampicillin or cloxacillin against MSSA with the FICIs of <0.5. The potential synergistic effect was also observed with lupinifolin plus ampicillin or cloxacillin against MRSA. However, the combination of lupinifolin plus vancomycin resulted in no interaction against MRSA. The combined effects of lupinifolin and ampicillin or cloxacillin against S. mutans were somewhat ambiguous with the borderline values of FICI of 0.5156 and 0.5625, respectively. Conclusion: Lupinifolin potentially plays a role as an antibacterial intensifier against some pathogenic gram-positive bacteria, particularly MSSA and MRSA. Nonetheless, further experiments are required to explain the precise mechanism of synergy.
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