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Articles by Saisamorn Lumyong
Total Records ( 7 ) for Saisamorn Lumyong
  Watee Kongbuntad , Kanokporn Saenphet , Pairote Wongputtisin , Chatchai Khanongnuch and Saisamorn Lumyong
  The purpose of this study was to evaluate the toxicology of crude xylanase enzymes prepared from T. aurantiacus SL16W in male Albino rats. Sixty rats aged 6 weeks old were randomly distributed into 4 groups of 15 rats. The 1st group was the untreated control while the 2nd (400 U), 3rd (2000 U) and 4th (4000 U) groups received the crude xylanase enzyme at dose levels of 400, 2000 and 4000 unit/kg body weight/day, respectively. After treatmemt body weight, organ weight, hematology and plasma chemistry were examined. The results indicated that oral force-feeding of the crude xylanase enzyme did not affect the body and organ weight of the rats. Hemoglobin values in the 2000 U group and hematocrit values in the 2000 U group were slightly decreased compared to the control group. The levels of Blood Urea Nitrogen (BUN), creatinine, aspartate Aminotransferase (AST) and alanine Aminotransferase (ALP) were not significantly different between the groups except that the ALT of the 200 U was significantly lower than the control group.
  Intira Thampayak , Naowarat Cheeptham , Wasu Pathom-Aree , Pimporn Leelapornpisid and Saisamorn Lumyong
  Two hundred and twenty-nine soil actinomycete strains were initially screened for extracellular biosurfactant activity by a drop-collapse method in Kim`s medium containing sesame oil as a sole source of carbon. Three isolates, namely S71, S72 and S177, were capable of biosurfactant production. Phenotypic and genotypic analysis strongly suggested that they were members of the genus Streptomyces. The isolates S71 and S177 were closely related to S. griseoflavus sharing 99% 16S rRNA gene similarities, whereas S72 was closely related to S. fradiae sharing only 98% 16S rRNA gene similarities suggesting that this may represent a novel species. The cell-free culture broth of the three isolates had emulsification activity and decreased surface tension. According to emulsification activity (E24) and surface tension values observed in the three isolates, Streptomyces sp. S72 was selected for biosurfactant production in larger scale. The cell-free culture broth of the isolate S72 was further extracted with chloroform:methanol (2:1) and two fractions were found positive in producing biosurfactants. To determine structure and molecular weight of the two positive fractions, the Nuclear Magnetic Resonance (NMR) spectroscopy and Mass Spectrometry (MS) will be carried out.
  Pairote Wongputtisin , Chartchai Khanongnuch , Puntipa Pongpiachan and Saisamorn Lumyong
  The improvement of antioxidant activity of soybean meal (SBM), an important protein source for monogastric animals, was studied by solid state fermentation. Fermented soybean meal (FSBM) was produced using pure culture of each Bacillus subtilis strain MR10 and TK8, B. natto and Rhizopus oligosporus that are accepted as GRAS microorganisms. It was found that the antioxidant activity in term of scavenging effect and reducing power was increased significantly at p<0.05. FSBM produced by Bacillus sp. and R. oligosporus showed percentage scavenging activity at the level of 70-99 and 49%, respectively, while only 25% of scavenging activity was found in SBM. Furthermore, the reducing power value (A700) of every FSBM and SBM was between 0.827-1.031 and 0.299, respectively.
  Weraphol Bhilabutra , Thongchai Techowisan , John F. Peberdy and Saisamorn Lumyong
  Periconia siamensis (strain CMUGE015) was isolated from leaves of the grass, Thysanoleana latifolia (Poaceae). It was antagonistic against the human pathogens Bacillus cereus, Listeria monocytogenes, MRSA (Methicillin Resistance Staphylococcus aureus) and Pseudomonas aeroginosa the causative agents of food borne disease, Listeriosis, skin infections and lung disease, respectively. Metabolits in both culture filtrates and crude extracts of the filtrates were inhibitory against the microbes tested. The major active ingredients from the culture filtrate were purified by silica gel column chromatography and identified to be Modiolide A, 5, 8-dihydroxy-10-methyl-5, 8, 9, 10-tetrahydro-2H-oxecin-2-one (Compound 1) and 4-Chromanone, 6-hydroxy-2-methyl- (5CI) (Compound 2) by IR, NMR and mass spectral (MS) data. Bioassays showed that both compounds had antibacterial activity against all the test bacteria. Minimum inhibitory concentrations were determined to be 50 μg mL-1 for compound 1 and 100 μg mL-1 for compound 2. This is the first report of the production of these two antibacterial metabolites a terrestrial endophytic fungus.
  Thongchai Taechowisan , Asawin Wanbanjob , Pittaya Tuntiwachwuttikul , Yuemao Shen and Saisamorn Lumyong
  Different extracts of Streptomyces aureofaciens CMUAc130 culture were studied as potential antifungal agents for selected phytopathogenic fungi. In a serial agar dilution method, crude ethyl acetate and 10% methanol in ethyl acetate extracts exhibited fungistatic activity against Aspergillus flavus, Colletotrichum musae, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Sclerotium rolfsii. Both ethyl acetate extract and 10% methanol in ethyl acetate extract were highly effective on all tested fungi, with Minimum Inhibitory Concentration (MIC) values ranging from 0.25 to 50 and 10 to 100 mg mL-1, respectively. The major active ingredients from those extracts were purified by silica gel column chromatography and identified to be 5, 7, 4`-trimethoxy-4-phenylcoumarin (1), 4`-hydroxy-5,7-dimethoxy-4-phenylcoumarin (2), 3`-Hydroxy-5,7,4`-trimethoxy-4-phenylcoumarin (3), 5,7,3`,4`-Tetramethoxy-4-phenylcoumarin (4) and 4`-hydroxy-5,7,3`-trimethoxy-4-phenylcoumarin (5) by NMR and mass spectral data, respectively. Five compounds (1 to 5) had activity against F. oxysporum with MICs of 0.30, 1.00, 0.40, 10.00 and 20.00 mg mL-1, respectively. Compounds 1, 2 and 3 also showed a synergistic effect when combined in different concentrations, displaying four times less concentration to reach complete inhibition in the growth of F. oxysporum.
  Jiraporn Nikomtat , Narumol Thongwai , Saisamorn Lumyong and Yingmanee Tragoolpua
  The effect of Cissus repanda Vahl. plant extract on herpes simplex virus type 1 and type 2 was investigated in this study. The cytotoxicity of dichloromethane and methanol extracts of C. repanda on GMK cells was determined by MTT assay. Non toxic concentrations were used in the study. Methanol extracts of C. repanda showed higher anti-HSV efficacy than dichloromethane extracts, by plaque reduction assay, although, HSV particles were directly inhibited by both extracts. Inhibition of HSV attachment, penetration and replication were also observed, after treatment of HSV with C. repanda extracts. The most affected stage was the attachment period. Moreover, C. repanda extracts inhibited replication of HSV-2 more than HSV-1.
  Ekachai Chukeatirote , Chularat Chainun , Apiradee Siengsubchart , Churairat Moukamnerd , Panuwan Chantawannakul , Saisamorn Lumyong , Nitaya Boontim and Primprao Thakang
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