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Articles by Sagar Grover
Total Records ( 2 ) for Sagar Grover
  Sagar Grover , Shaily Rubina Tirkey , Veeramallegowda , Saroj Yadav and G. Sibi
  Background and Objective: The mutualistic interaction between microalgae and bacteria when co-culturing aid each other by promoting growth. This work was planned to determine the benefits of co-culturing Chlorella vulgaris with Nitrobacter sp. in terms of enhanced microalgal biomass and lipid production. Materials and Methods: Different growth media viz., Bristol media, synthetic waste water and dilutions of waste water were used for co-culturing for a period of 16 days. Enhancement of microalgal growth in terms of growth rate, biomass, protein, carbohydrates, chlorophyll and total lipid content in microalgae were determined at regular intervals and at the end of cultivation period. Results: Specific growth rate was promoted in co-culture after 4th day of cultivation whereas monoculture has resulted in highest growth rate after 13th day of cultivation. Protein and carbohydrate contents of mono- and co-cultured C. vulgaris were 20.03 and 9.413 μg mg1, respectively in 50% sewage water. There was a down trend in dissolved oxygen levels when the microalgae were co-cultured with bacteria. Biomass productivity was 0.0371 g L1/day in monoculture after 16th day of cultivation and was 0.0285 g L1/day after 4 days of cultivation as co-culture. Highest lipid content of 20.69% was observed in monoculture and the co-culture has resulted in 17.93%. Conclusion: The results indicated that co-culturing of C. vulgaris with Nitrobacter resulted in enhanced growth promotion as evidenced by increased cellular composition and biomass content. This interaction could be utilized in enhancing microalgal biomass, especially by replacing nitrogen fertilizers in the growth medium.
  Sagar Grover , Shaily Rubina , Veera Malle Gowda and G. Sibi
  Background and Objective: Inorganic fertilizers are used to cultivate microalgae causing adverse environmental effects and increase the cost of microalgal cultivation. The objective of this study was to find a cheap, nutrient rich alternate to cultivate freshwater microalgae. Materials and Methods: Vermiwash at a concentrations of 25, 50, 75 and 100% were used to cultivate microalgae isolated from fresh water habitats. Specific growth rate and biomass concentrations were determined to evaluate the effect of vermiwash medium in comparison with Bold’s basal medium. Results: A total of 13 microalgae that belonged to the family Cyanophyceae and Chlorophyceae were isolated. Growth rate and biomass of microalgae were increased with higher concentrations when the vermiwash was used at a concentration of 25, 50 and 75%. At the same time, both the parameters were declined in undiluted vermiwash indicating the algal growth inhibition under high levels of nutrients. Conclusion: Utilization of vermiwash is feasible to cultivate fresh water microalgae thereby fulfilling the nutrient requirements and reducing the use of inorganic fertilizers.
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