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Articles by Safia Ahmad
Total Records ( 2 ) for Safia Ahmad
  Ali Abbas Qazilbash , Rani Faryal , Kokab Batool Naqvi , Safia Ahmad and Abdul Hameed
  This study was designed to ascertain the effectiveness of isolated indigenous Bacillus species to remove chromium from industrially polluted effluents, through a series of effluent biotreatment regimen. Using microbiological techniques, 6 Bacillus strains were isolated, characterized and labeled QIP 1-6. All strains displayed maximum growth on media with a Cr concentration of 400 ppm. Strains QIP 1 and 5 showed maximum resistance to chromium showing moderate growth at 900 ppm of Cr. Following optimization, strain QIP 1 removed 46.81% of the Cr6+ and QIP 5 removed 42.50% of the chromium at the same concentration over the same incubation period, as determined through atomic absorption spectroscopy. Following different biotreatment regimens, biotreatment C1-filtered effluent inoculated with strain QIP 1, incubated under optimum conditions, showed maximum Cr6+ removal (10.29%) after 48 h. Interestingly, the second highest removal of Cr6+ (8.94%) occurred in the untreated, unfiltered raw effluent (D) after incubation for 48 h. Significant (p<0.05) strong positive correlation patterns emerged between these two regimen, as well as between regimen B 1-filtered effluent inoculated with strain QIP 1, but incubated under un-optimized conditions-and regimen D. The study revealed that intrinsic bioremediation does occur naturally and that it is quite possible for a consortium of Bacillus species to work more effectively at removing the HM from the contaminated sites, than a single isolate, thereby reducing the labor intensive work involved.
  Abdul Haleem Shah , Abdul Hameed , Safia Ahmad and Gul Majid Khan
  The objective of this study was to improve the L-lysine production by Corynebacterium glutamicum. For this purpose culture conditions for the lysine fermentation by the bacteria were optimized. In this regard the role of various physical and nutritional parameters was examined. The culture was incubated in a 500 ml Erlenmeyer flask in a rotatory shaking incubator at 200 rpm. The appropriate conditions were obtained only when 50 ml medium was charged for fermentation at 30 oC, pH 7.5 and at 10% inoculum size. The finally selected medium per 100 ml distilled water formulated was 10 g glucose, 2.5 g ammonium sulfate, 2.0 g calcium carbonate, 0.5 g bactocasamino acid, 20 μg thiamine hydrochlorid, 5 μg D-biotin, 0.1 g potassium dihydrogen phosphate, 0.05 g magnesium sulfate heptahydrate, 0.2 mg ferrous sulfate heptahydrate and 0.2 mg manganese chloride tetrahydrate. The optimized culture conditions resulted into elevated amount of L-lysine.
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