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Articles by Safa A. Sherfi
Total Records ( 2 ) for Safa A. Sherfi
  Safa A. Sherfi , Hamid A. Dirar , Badr E. Hago , Mohamed E. Ahmed , Hassan A. Musa , Hassan Abu Aisha and Imadeldin E. Aradaib
  The potential of the Polymerase Chain Reaction (PCR), as a means of detecting Escherichia coli (E. coli) DNA in suspected environmental samples, was studied. Using a pair of outer primers P1 and P2, selected from uidA gene, which encodes E. coli glucuronidase, the PCR-based assay resulted in amplification of a 486 base pair (bp) PCR product. E. coli strains from different environmental sources including recycled and drinking water as well as stagnant water were detected by this nested PCR-based assay. Amplification products were visualized on ethidium bromide-stained agarose gel. The sensitivity of the PCR assay was 100 fg of bacterial DNA with ethidium bromide-stained agarose gels. Using a pair of internal (nested) primers P3 and P4, the nested PCR produced a 186 bp PCR product. The nested PCR increased the sensitivity of the PCR assay by 1,000 times and specific PCR products were detected from 0.1 fg of bacterial DNA. Amplification product was not detected when the nested PCR-based assay was applied to DNA from other related bacteria including, Salmonella, Pseudomonas and Proteus or nucleic acid-free water. Application of this nested PCR-based assay to environmental samples resulted in direct detection of E. coli DNA from sewage water, tap water, drinking water at Shambat Campus, University of Khartoum, Sudan. This nested PCR-based assay should provide a rapid, sensitive and specific assay for direct detection and quantification of E. coli in environmental samples suspected to contain the organism.
  Mohamed E. Ahmed , Imadeldin E. Aradaib , AbdelRahim E. Karrar , Badr E. Hago and Safa A. Sherfi
  Two patients (adult females) were admitted to Ahmed Gasim Medical Hospital, Khartoum North, Sudan, with history of severe combined mitral regurgitation and stenosis. The patients were referred to the thoracic surgery unit for mitral valvectomy and valve replacement. Both patients received early empirical prophylactic antibiotic therapy half an hour before as well as post operatively. However, they developed fever shortly after the operation. Blood samples were collected and submitted to the diagnostic laboratory for conventional and molecular microbiological examinations. Conventional bacteriological examination revealed that blood cultures were negative for any bacterial growth. However, the Polymerase Chain Reaction (PCR) detected a 486 bp PCR product specific for E. coli. The identity of the nucleic acid sequence was confirmed by nested amplification of a 186 bp PCR product from the primary PCR product. The scientific data presented in this study indicated that PCR provides a rapid method for detection of E. coli during bacteraemia, irrespective of their viability. However, conventional bacterial isolation methods failed to diagnose E. coli infection in patients receiving high doses of antibiotics.
 
 
 
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