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Articles by Saber Qanbari
Total Records ( 2 ) for Saber Qanbari
  Reza Halabian , Morad Pasha Eskandari Nasab , Mohammad Reza Nassiry , Ali Reza Heravi Mossavi , Seyed Abolfazl Hosseini and Saber Qanbari
  In this study, DNA isolated from blood samples of Iranian Holstein cows (N = 120) and PCR products (156 bp) were analyzed by SSCP technique with MDE gel. Then 4 patterns (Z1, Z2, ...Z4) of provided by SSCP were sequenced and then using POLYPHRED 5.04 software, 4 SNPs were detected in positions 6237, 6263, 6268 and 6297. The genotypes were TC and TG in position 6237, TT in position 6263, AG in position 6268 and AC in position 6297. From of detected SNPs, they that located in positions 6237 and 6268 caused change in the amino acids sequence of protein. These data provide evidence that bPRL gene is a good polymorphic source for single nucleotides that can be used for association with milk yield and investigate whether mutations in this sequences might be responsible for quantitative variations in milk yield and composition.
  Morteza Pashmi , Saber Qanbari , Seyed Ali Ghorashi and Abdolreza Salehi
  Major Histocompatibility Complex (MHC) class II locus DRB3 was investigated by PCR based restriction fragment length polymorphism (PCR-RFLP) assay. A total of 262 Holstein cows participating in the national recording system were sampled from 10 herds. A two-step polymerase chain reaction was carried out in order to amplify a 284 base-pair fragment of exon 2 of the target gene. Second PCR products were treated with three restriction endonucleas enzymes RsaI, BstYI and HaeIII. Digested fragments were analyzed by polyacrylamid gel electrophoresis. Twenty-eight BoLA-DRB3 alleles were identified. Identified alleles are: BoLA-DRB3.2 *3, *6, *7, *8, *9, *10, *11, *12, *13, *14, *15, *16, 20, *21, *22, *23, *24, *25, *26, *27, *28, *32, *36, *37, *40, *51, *iaa and *ibb. The BoLA-DRB3.2*40 allele that was observed in this study has not been reported previously. The calculated frequencies were as follows: 2.29, 1.34, 0.19, 14.5, 0.38, 3.05, 12.21, 1.34, 2.29, 1.34, 2.48, 9.16, 0.95, 0.77, 6.68, 9.16, 17.94, 1.15, 0.57, 1.15, 0.95, 0.57, 0.38, 1.91, 0.38, 5.73, 0.19 and 0.95% respectively. The six most frequently observed alleles (BoLA-DRB3.2 *8, *11, *16, *22, *23 and *24) accounted for 69.65% of the alleles in these 10 herds. The results of this study confirm the allelic distribution of six most frequent alleles in Holstein population`s worldwide.
 
 
 
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